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Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis
PURPOSE: Breast cancer (BC) is characterized by concealed onset, delayed diagnosis, and high fatality rates making it particularly dangerous to patients’ health. The purpose of this study was to use comprehensive bioinformatics analysis and experimental verification to find a new biomarker for BC di...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451442/ https://www.ncbi.nlm.nih.gov/pubmed/34616615 http://dx.doi.org/10.7717/peerj.12147 |
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author | Liu, Min Mo, Fei Song, Xiaohan He, Yun Yuan, Yan Yan, Jiaoyan Yang, Ye Huang, Jian Zhang, Shu |
author_facet | Liu, Min Mo, Fei Song, Xiaohan He, Yun Yuan, Yan Yan, Jiaoyan Yang, Ye Huang, Jian Zhang, Shu |
author_sort | Liu, Min |
collection | PubMed |
description | PURPOSE: Breast cancer (BC) is characterized by concealed onset, delayed diagnosis, and high fatality rates making it particularly dangerous to patients’ health. The purpose of this study was to use comprehensive bioinformatics analysis and experimental verification to find a new biomarker for BC diagnosis. METHODS: We comprehensively analyzed microRNA (miRNA) and mRNA expression profiles from the Gene Expression Omnibus (GEO) and screened out differentially-expressed (DE) miRNAs and mRNAs. We used the miRNet website to predict potential DE-miRNA target genes. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID), we performed Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on overlapping potential target genes and DE-mRNAs. The protein-protein interaction (PPI) network was then established. The miRNA-mRNA regulatory network was constructed using Cytoscape and the analysis results were visualized. We verified the expression of the most up-regulated DE-miRNA using reverse transcription and a quantitative polymerase chain reaction in BC tissue. The diagnostic value of the most up-regulated DE-miRNA was further explored across three levels: plasma-derived exosomes, cells, and cell exosomes. RESULTS: Our comprehensive bioinformatics analysis and experimental results showed that hsa-miR-21-5p was significantly up-regulated in BC tissue, cells, and exosomes. Our results also revealed that tumor-derived hsa-miR-21-5p could be packaged in exosomes and released into peripheral blood. Additionally, when evaluating the diagnostic value of plasma exosomal hsa-miR-21-5p, we found that it was significantly up-regulated in BC patients. Receiver operating characteristic (ROC) analysis also confirmed that hsa-miR-21-5p could effectively distinguish healthy people from BC patients. The sensitivity and specificity were 86.7% and 93.3%, respectively. CONCLUSION: This study’s results showed that plasma exosomal hsa-miR-21-5p could be used as a biomarker for BC diagnosis. |
format | Online Article Text |
id | pubmed-8451442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84514422021-10-05 Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis Liu, Min Mo, Fei Song, Xiaohan He, Yun Yuan, Yan Yan, Jiaoyan Yang, Ye Huang, Jian Zhang, Shu PeerJ Bioinformatics PURPOSE: Breast cancer (BC) is characterized by concealed onset, delayed diagnosis, and high fatality rates making it particularly dangerous to patients’ health. The purpose of this study was to use comprehensive bioinformatics analysis and experimental verification to find a new biomarker for BC diagnosis. METHODS: We comprehensively analyzed microRNA (miRNA) and mRNA expression profiles from the Gene Expression Omnibus (GEO) and screened out differentially-expressed (DE) miRNAs and mRNAs. We used the miRNet website to predict potential DE-miRNA target genes. Using the Database for Annotation, Visualization and Integrated Discovery (DAVID), we performed Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses on overlapping potential target genes and DE-mRNAs. The protein-protein interaction (PPI) network was then established. The miRNA-mRNA regulatory network was constructed using Cytoscape and the analysis results were visualized. We verified the expression of the most up-regulated DE-miRNA using reverse transcription and a quantitative polymerase chain reaction in BC tissue. The diagnostic value of the most up-regulated DE-miRNA was further explored across three levels: plasma-derived exosomes, cells, and cell exosomes. RESULTS: Our comprehensive bioinformatics analysis and experimental results showed that hsa-miR-21-5p was significantly up-regulated in BC tissue, cells, and exosomes. Our results also revealed that tumor-derived hsa-miR-21-5p could be packaged in exosomes and released into peripheral blood. Additionally, when evaluating the diagnostic value of plasma exosomal hsa-miR-21-5p, we found that it was significantly up-regulated in BC patients. Receiver operating characteristic (ROC) analysis also confirmed that hsa-miR-21-5p could effectively distinguish healthy people from BC patients. The sensitivity and specificity were 86.7% and 93.3%, respectively. CONCLUSION: This study’s results showed that plasma exosomal hsa-miR-21-5p could be used as a biomarker for BC diagnosis. PeerJ Inc. 2021-09-17 /pmc/articles/PMC8451442/ /pubmed/34616615 http://dx.doi.org/10.7717/peerj.12147 Text en ©2021 Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Liu, Min Mo, Fei Song, Xiaohan He, Yun Yuan, Yan Yan, Jiaoyan Yang, Ye Huang, Jian Zhang, Shu Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis |
title | Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis |
title_full | Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis |
title_fullStr | Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis |
title_full_unstemmed | Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis |
title_short | Exosomal hsa-miR-21-5p is a biomarker for breast cancer diagnosis |
title_sort | exosomal hsa-mir-21-5p is a biomarker for breast cancer diagnosis |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451442/ https://www.ncbi.nlm.nih.gov/pubmed/34616615 http://dx.doi.org/10.7717/peerj.12147 |
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