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Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients

To assess the m(6)A methylome in the anterior capsule of the lens of high myopia patients. MeRIP-seq and RNA-seq were performed to identify differences in the m(6)A methylomes and gene expression between anterior capsule of the lens of simple nuclear cataract patients (N) and nuclear cataract patien...

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Autores principales: Wen, Kai, Zhang, Yan, Li, Yahong, Wang, Qing, Sun, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451460/
https://www.ncbi.nlm.nih.gov/pubmed/33108260
http://dx.doi.org/10.1080/15592294.2020.1834917
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author Wen, Kai
Zhang, Yan
Li, Yahong
Wang, Qing
Sun, Jing
author_facet Wen, Kai
Zhang, Yan
Li, Yahong
Wang, Qing
Sun, Jing
author_sort Wen, Kai
collection PubMed
description To assess the m(6)A methylome in the anterior capsule of the lens of high myopia patients. MeRIP-seq and RNA-seq were performed to identify differences in the m(6)A methylomes and gene expression between anterior capsule of the lens of simple nuclear cataract patients (N) and nuclear cataract patients with high myopia (G). Expression of m(6)A-related enzymes was confirmed by quantitative real-time-PCR. ALKBH5 was downregulated in G. The observed m(6)A peak was identical to the conserved RRACH gmotif and was markedly correlated with two distinct coordinates. Differentially methylated genes were enriched in some pathways regulating the formation of extracellular matrix. These findings suggest that upregulation of m(6)A methylation may change fundus anatomy by regulating the composition of the extracellular matrix through encoding protein.
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spelling pubmed-84514602021-09-21 Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients Wen, Kai Zhang, Yan Li, Yahong Wang, Qing Sun, Jing Epigenetics Research Paper To assess the m(6)A methylome in the anterior capsule of the lens of high myopia patients. MeRIP-seq and RNA-seq were performed to identify differences in the m(6)A methylomes and gene expression between anterior capsule of the lens of simple nuclear cataract patients (N) and nuclear cataract patients with high myopia (G). Expression of m(6)A-related enzymes was confirmed by quantitative real-time-PCR. ALKBH5 was downregulated in G. The observed m(6)A peak was identical to the conserved RRACH gmotif and was markedly correlated with two distinct coordinates. Differentially methylated genes were enriched in some pathways regulating the formation of extracellular matrix. These findings suggest that upregulation of m(6)A methylation may change fundus anatomy by regulating the composition of the extracellular matrix through encoding protein. Taylor & Francis 2020-10-27 /pmc/articles/PMC8451460/ /pubmed/33108260 http://dx.doi.org/10.1080/15592294.2020.1834917 Text en © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.
spellingShingle Research Paper
Wen, Kai
Zhang, Yan
Li, Yahong
Wang, Qing
Sun, Jing
Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients
title Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients
title_full Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients
title_fullStr Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients
title_full_unstemmed Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients
title_short Comprehensive analysis of transcriptome-wide m(6)A methylome in the anterior capsule of the lens of high myopia patients
title_sort comprehensive analysis of transcriptome-wide m(6)a methylome in the anterior capsule of the lens of high myopia patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451460/
https://www.ncbi.nlm.nih.gov/pubmed/33108260
http://dx.doi.org/10.1080/15592294.2020.1834917
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