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Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids
Chemically defined media are reconstituted batchwise and stored in hold tanks until use. To avoid large hold tanks and batchwise production of media, we developed continuous on‐demand reconstitutions directly from solids consisting of a hopper and a screw conveyor capable of feeding dry powdered med...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451748/ https://www.ncbi.nlm.nih.gov/pubmed/33656168 http://dx.doi.org/10.1002/bit.27738 |
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author | Komuczki, Daniel Dutra, Gregory Gstöttner, Christoph Dominguez‐Vega, Elena Jungbauer, Alois Satzer, Peter |
author_facet | Komuczki, Daniel Dutra, Gregory Gstöttner, Christoph Dominguez‐Vega, Elena Jungbauer, Alois Satzer, Peter |
author_sort | Komuczki, Daniel |
collection | PubMed |
description | Chemically defined media are reconstituted batchwise and stored in hold tanks until use. To avoid large hold tanks and batchwise production of media, we developed continuous on‐demand reconstitutions directly from solids consisting of a hopper and a screw conveyor capable of feeding dry powdered media with the required precision ±5% at low dosing rates of 0.171 g min(−1). A commercially available dry powdered cell culture medium was continuously fed over a duration of 12 h into a mixer which was connected to a UV‐cell for monitoring and the media were compared to a batchwise production. A comparable amino acid, carbohydrate, and osmolality profile to a batchwise reconstitution could be obtained. Cell cultivation showed comparable performance of batch and continuous reconstitution for two CHO cell lines producing the antibodies adalimumab and trastuzumab on a small and benchtop scale. In‐depth analysis of the produced antibodies showed the same glycosylation pattern, other posttranslational profiles such as methionine oxidation and deamidation compared to batchwise reconstitution. Therefore, we conclude a continuous reconstitution of the medium results in the same quality of the product. A continuous on‐demand media reconstitution will impact the supply chain and significantly reduce the floor space necessary for preparation and storage. |
format | Online Article Text |
id | pubmed-8451748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84517482021-09-27 Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids Komuczki, Daniel Dutra, Gregory Gstöttner, Christoph Dominguez‐Vega, Elena Jungbauer, Alois Satzer, Peter Biotechnol Bioeng ARTICLES Chemically defined media are reconstituted batchwise and stored in hold tanks until use. To avoid large hold tanks and batchwise production of media, we developed continuous on‐demand reconstitutions directly from solids consisting of a hopper and a screw conveyor capable of feeding dry powdered media with the required precision ±5% at low dosing rates of 0.171 g min(−1). A commercially available dry powdered cell culture medium was continuously fed over a duration of 12 h into a mixer which was connected to a UV‐cell for monitoring and the media were compared to a batchwise production. A comparable amino acid, carbohydrate, and osmolality profile to a batchwise reconstitution could be obtained. Cell cultivation showed comparable performance of batch and continuous reconstitution for two CHO cell lines producing the antibodies adalimumab and trastuzumab on a small and benchtop scale. In‐depth analysis of the produced antibodies showed the same glycosylation pattern, other posttranslational profiles such as methionine oxidation and deamidation compared to batchwise reconstitution. Therefore, we conclude a continuous reconstitution of the medium results in the same quality of the product. A continuous on‐demand media reconstitution will impact the supply chain and significantly reduce the floor space necessary for preparation and storage. John Wiley and Sons Inc. 2021-03-25 2021-09 /pmc/articles/PMC8451748/ /pubmed/33656168 http://dx.doi.org/10.1002/bit.27738 Text en © 2021 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | ARTICLES Komuczki, Daniel Dutra, Gregory Gstöttner, Christoph Dominguez‐Vega, Elena Jungbauer, Alois Satzer, Peter Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids |
title | Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids |
title_full | Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids |
title_fullStr | Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids |
title_full_unstemmed | Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids |
title_short | Media on‐demand: Continuous reconstitution of a chemically defined media directly from solids |
title_sort | media on‐demand: continuous reconstitution of a chemically defined media directly from solids |
topic | ARTICLES |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451748/ https://www.ncbi.nlm.nih.gov/pubmed/33656168 http://dx.doi.org/10.1002/bit.27738 |
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