Next generation sequencing in cytology

The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high‐quality DNA for NGS analysis, especially for predicting patients' response to targeted treatmen...

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Autores principales: Pisapia, Pasquale, Pepe, Francesco, Sgariglia, Roberta, Nacchio, Mariantonia, Russo, Gianluca, Conticelli, Floriana, Girolami, Ilaria, Eccher, Albino, Bellevicine, Claudio, Vigliar, Elena, Malapelle, Umberto, Troncone, Giancarlo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Reviews
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451925/
https://www.ncbi.nlm.nih.gov/pubmed/33792981
http://dx.doi.org/10.1111/cyt.12974
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author Pisapia, Pasquale
Pepe, Francesco
Sgariglia, Roberta
Nacchio, Mariantonia
Russo, Gianluca
Conticelli, Floriana
Girolami, Ilaria
Eccher, Albino
Bellevicine, Claudio
Vigliar, Elena
Malapelle, Umberto
Troncone, Giancarlo
author_facet Pisapia, Pasquale
Pepe, Francesco
Sgariglia, Roberta
Nacchio, Mariantonia
Russo, Gianluca
Conticelli, Floriana
Girolami, Ilaria
Eccher, Albino
Bellevicine, Claudio
Vigliar, Elena
Malapelle, Umberto
Troncone, Giancarlo
author_sort Pisapia, Pasquale
collection PubMed
description The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high‐quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre‐analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre‐analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field.
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spelling pubmed-84519252021-09-27 Next generation sequencing in cytology Pisapia, Pasquale Pepe, Francesco Sgariglia, Roberta Nacchio, Mariantonia Russo, Gianluca Conticelli, Floriana Girolami, Ilaria Eccher, Albino Bellevicine, Claudio Vigliar, Elena Malapelle, Umberto Troncone, Giancarlo Cytopathology Reviews The application of next generation sequencing (NGS) technology to cytological samples has significantly modified molecular cytopathology practice. Cytological samples represent a valid source of high‐quality DNA for NGS analysis, especially for predicting patients' response to targeted treatments and for refining the risk of malignancy in indeterminate cytological diagnoses. However, several pre‐analytical factors may influence the reliability of NGS clinical analysis. Here, we briefly review the challenges of NGS in cytology practice, focusing on those pre‐analytical factors that may negatively affect NGS success rates and routine diagnostic applications. Finally, we address the future directions of the field. John Wiley and Sons Inc. 2021-04-01 2021-09 /pmc/articles/PMC8451925/ /pubmed/33792981 http://dx.doi.org/10.1111/cyt.12974 Text en © 2021 The Authors. Cytopathology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Reviews
Pisapia, Pasquale
Pepe, Francesco
Sgariglia, Roberta
Nacchio, Mariantonia
Russo, Gianluca
Conticelli, Floriana
Girolami, Ilaria
Eccher, Albino
Bellevicine, Claudio
Vigliar, Elena
Malapelle, Umberto
Troncone, Giancarlo
Next generation sequencing in cytology
title Next generation sequencing in cytology
title_full Next generation sequencing in cytology
title_fullStr Next generation sequencing in cytology
title_full_unstemmed Next generation sequencing in cytology
title_short Next generation sequencing in cytology
title_sort next generation sequencing in cytology
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8451925/
https://www.ncbi.nlm.nih.gov/pubmed/33792981
http://dx.doi.org/10.1111/cyt.12974
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