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A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis

Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we a...

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Autores principales: Rankothgedera, Sakuni, Atukorala, Inoshi, Fernando, Chandrika, Munidasa, Duminda, Wijayaratne, Lalith, Udagama, Preethi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452079/
https://www.ncbi.nlm.nih.gov/pubmed/34543351
http://dx.doi.org/10.1371/journal.pone.0257507
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author Rankothgedera, Sakuni
Atukorala, Inoshi
Fernando, Chandrika
Munidasa, Duminda
Wijayaratne, Lalith
Udagama, Preethi
author_facet Rankothgedera, Sakuni
Atukorala, Inoshi
Fernando, Chandrika
Munidasa, Duminda
Wijayaratne, Lalith
Udagama, Preethi
author_sort Rankothgedera, Sakuni
collection PubMed
description Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we aimed to differentially identify potential serum immunologic diagnostic markers of pKOA and of sKOA. This case control study used 46 KOA patients (pKOA, n = 30; sKOA, n = 16), and 60 age, gender matched controls (normal healthy, n = 30; systemic lupus erythematosus [SLE] disease controls, n = 30) where serum was assayed for cytokines (TNF-α, IL-1β, IL-6, IL-10) and nitric oxide derivatives (NOx). Sandwich ELISA assessed cytokine levels, while the ‘Griess assay’ quantified NOx levels. The diagnostic accuracy of optimal marker combinations was evaluated by the CombiROC web tool. Compared with pKOA, sKOA serum displayed significantly elevated levels of pro inflammatory cytokines (TNF-α, IL-1β, IL-6) with a concurrent decrease in the anti-inflammatory cytokine, IL-10 (P<0.05). This was reiterated by significantly higher Th1:Th2 (TNF-α: IL-10) serum cytokine ratio observed in sKOA compared to that of pKOA. The CombiROC curves identified TNF-α, IL-1β, IL-6 and NOx as the best performing panel of potential diagnostic markers to discriminate pKOA from control groups (~97% accuracy, 90% Sensitivity [SE] and 98% specificity [SP]), while TNF-α, IL-1β and IL-6 discriminated sKOA from control groups (~100% accuracy, 100% SE, and 98% SP). The study identified discrete serum immune biomarker panels to differentiate between pKOA (TNF-α, IL-1β, IL-6 and NOx) and sKOA (TNF-α, IL-1β and IL-6). These findings may assist in developing distinct therapeutic agents for the two types of KOA.
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spelling pubmed-84520792021-09-21 A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis Rankothgedera, Sakuni Atukorala, Inoshi Fernando, Chandrika Munidasa, Duminda Wijayaratne, Lalith Udagama, Preethi PLoS One Research Article Inflammation contributes to knee osteoarthritis (KOA) where many immunological mediators participate in its initiation and progression. Most clinicians manage primary (pKOA) and secondary osteoarthritis (sKOA) alike. Whether immunological profiles of pKOA and sKOA differ remains obscure. Hence, we aimed to differentially identify potential serum immunologic diagnostic markers of pKOA and of sKOA. This case control study used 46 KOA patients (pKOA, n = 30; sKOA, n = 16), and 60 age, gender matched controls (normal healthy, n = 30; systemic lupus erythematosus [SLE] disease controls, n = 30) where serum was assayed for cytokines (TNF-α, IL-1β, IL-6, IL-10) and nitric oxide derivatives (NOx). Sandwich ELISA assessed cytokine levels, while the ‘Griess assay’ quantified NOx levels. The diagnostic accuracy of optimal marker combinations was evaluated by the CombiROC web tool. Compared with pKOA, sKOA serum displayed significantly elevated levels of pro inflammatory cytokines (TNF-α, IL-1β, IL-6) with a concurrent decrease in the anti-inflammatory cytokine, IL-10 (P<0.05). This was reiterated by significantly higher Th1:Th2 (TNF-α: IL-10) serum cytokine ratio observed in sKOA compared to that of pKOA. The CombiROC curves identified TNF-α, IL-1β, IL-6 and NOx as the best performing panel of potential diagnostic markers to discriminate pKOA from control groups (~97% accuracy, 90% Sensitivity [SE] and 98% specificity [SP]), while TNF-α, IL-1β and IL-6 discriminated sKOA from control groups (~100% accuracy, 100% SE, and 98% SP). The study identified discrete serum immune biomarker panels to differentiate between pKOA (TNF-α, IL-1β, IL-6 and NOx) and sKOA (TNF-α, IL-1β and IL-6). These findings may assist in developing distinct therapeutic agents for the two types of KOA. Public Library of Science 2021-09-20 /pmc/articles/PMC8452079/ /pubmed/34543351 http://dx.doi.org/10.1371/journal.pone.0257507 Text en © 2021 Rankothgedera et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Rankothgedera, Sakuni
Atukorala, Inoshi
Fernando, Chandrika
Munidasa, Duminda
Wijayaratne, Lalith
Udagama, Preethi
A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
title A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
title_full A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
title_fullStr A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
title_full_unstemmed A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
title_short A potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
title_sort potential diagnostic serum immunological marker panel to differentiate between primary and secondary knee osteoarthritis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452079/
https://www.ncbi.nlm.nih.gov/pubmed/34543351
http://dx.doi.org/10.1371/journal.pone.0257507
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