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Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections

Staphylococcus aureus is one of the main pathogens isolated from diabetic foot infections (DFI). The purpose of this study was to evaluate the importance of the persistence of S. aureus in this environment and the possible modifications of the bacterial genome content over time. Molecular typing of...

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Autores principales: Lavigne, Jean-Philippe, Hosny, Michel, Dunyach-Remy, Catherine, Boutet-Dubois, Adeline, Schuldiner, Sophie, Cellier, Nicolas, Yahiaoui-Martinez, Alex, Molle, Virginie, La Scola, Bernard, Marchandin, Hélène, Sotto, Albert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452158/
https://www.ncbi.nlm.nih.gov/pubmed/34552578
http://dx.doi.org/10.3389/fmicb.2021.741406
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author Lavigne, Jean-Philippe
Hosny, Michel
Dunyach-Remy, Catherine
Boutet-Dubois, Adeline
Schuldiner, Sophie
Cellier, Nicolas
Yahiaoui-Martinez, Alex
Molle, Virginie
La Scola, Bernard
Marchandin, Hélène
Sotto, Albert
author_facet Lavigne, Jean-Philippe
Hosny, Michel
Dunyach-Remy, Catherine
Boutet-Dubois, Adeline
Schuldiner, Sophie
Cellier, Nicolas
Yahiaoui-Martinez, Alex
Molle, Virginie
La Scola, Bernard
Marchandin, Hélène
Sotto, Albert
author_sort Lavigne, Jean-Philippe
collection PubMed
description Staphylococcus aureus is one of the main pathogens isolated from diabetic foot infections (DFI). The purpose of this study was to evaluate the importance of the persistence of S. aureus in this environment and the possible modifications of the bacterial genome content over time. Molecular typing of S. aureus isolates cultured from patients with the same DFI over a 7-year study revealed a 25% rate of persistence of this species in 48 patients, with a short median persistence time of 12weeks (range: 4–52weeks). Non-specific clonal complexes were linked to this persistence. During the follow-up, bla genes were acquired in three cases, whereas some virulence markers were lost in all cases after a long period of colonization (21.5weeks). Only one patient (2%) had a long-term persistence of 48weeks. The genome sequencing of a clonal pair of early/late strains isolated in this patient showed mutations in genes encoding bacterial defence and two-component signal transduction systems. Although, this study suggests that the long-term persistence of S. aureus in DFI is a rare event, genomic evolution is observed, highlighting the low adaptive ability of S. aureus to the specific environment and stressful conditions of diabetic foot ulcers. These results provide the basis for better understanding of S. aureus dynamics during persistent colonization in chronic wounds.
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spelling pubmed-84521582021-09-21 Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections Lavigne, Jean-Philippe Hosny, Michel Dunyach-Remy, Catherine Boutet-Dubois, Adeline Schuldiner, Sophie Cellier, Nicolas Yahiaoui-Martinez, Alex Molle, Virginie La Scola, Bernard Marchandin, Hélène Sotto, Albert Front Microbiol Microbiology Staphylococcus aureus is one of the main pathogens isolated from diabetic foot infections (DFI). The purpose of this study was to evaluate the importance of the persistence of S. aureus in this environment and the possible modifications of the bacterial genome content over time. Molecular typing of S. aureus isolates cultured from patients with the same DFI over a 7-year study revealed a 25% rate of persistence of this species in 48 patients, with a short median persistence time of 12weeks (range: 4–52weeks). Non-specific clonal complexes were linked to this persistence. During the follow-up, bla genes were acquired in three cases, whereas some virulence markers were lost in all cases after a long period of colonization (21.5weeks). Only one patient (2%) had a long-term persistence of 48weeks. The genome sequencing of a clonal pair of early/late strains isolated in this patient showed mutations in genes encoding bacterial defence and two-component signal transduction systems. Although, this study suggests that the long-term persistence of S. aureus in DFI is a rare event, genomic evolution is observed, highlighting the low adaptive ability of S. aureus to the specific environment and stressful conditions of diabetic foot ulcers. These results provide the basis for better understanding of S. aureus dynamics during persistent colonization in chronic wounds. Frontiers Media S.A. 2021-09-06 /pmc/articles/PMC8452158/ /pubmed/34552578 http://dx.doi.org/10.3389/fmicb.2021.741406 Text en Copyright © 2021 Lavigne, Hosny, Dunyach-Remy, Boutet-Dubois, Schuldiner, Cellier, Yahiaoui-Martinez, Molle, La Scola, Marchandin and Sotto. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Lavigne, Jean-Philippe
Hosny, Michel
Dunyach-Remy, Catherine
Boutet-Dubois, Adeline
Schuldiner, Sophie
Cellier, Nicolas
Yahiaoui-Martinez, Alex
Molle, Virginie
La Scola, Bernard
Marchandin, Hélène
Sotto, Albert
Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections
title Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections
title_full Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections
title_fullStr Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections
title_full_unstemmed Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections
title_short Long-Term Intrahost Evolution of Staphylococcus aureus Among Diabetic Patients With Foot Infections
title_sort long-term intrahost evolution of staphylococcus aureus among diabetic patients with foot infections
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452158/
https://www.ncbi.nlm.nih.gov/pubmed/34552578
http://dx.doi.org/10.3389/fmicb.2021.741406
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