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Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
BACKGROUND: Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulat...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452476/ https://www.ncbi.nlm.nih.gov/pubmed/34585053 http://dx.doi.org/10.1002/ags3.12460 |
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author | Takasu, Chie Miyazaki, Katsuki Yoshikawa, Kozo Nishi, Masaaki Tokunaga, Takuya Kashihara, Hideya Yoshimoto, Toshiaki Ogawa, Hirohisa Morine, Yuji Shimada, Mitsuo |
author_facet | Takasu, Chie Miyazaki, Katsuki Yoshikawa, Kozo Nishi, Masaaki Tokunaga, Takuya Kashihara, Hideya Yoshimoto, Toshiaki Ogawa, Hirohisa Morine, Yuji Shimada, Mitsuo |
author_sort | Takasu, Chie |
collection | PubMed |
description | BACKGROUND: Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulates the immune response during BT by inducing PD‐1 expression in Peyer's patches. METHODS: Eighteen male Wistar rats were divided into four groups: a control group; a control + TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d; a BT group, given CPT‐11 250 mg/kg intra‐peritoneal for 2 d; and a TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d with CPT‐11 250 mg/kg intra‐peritoneal on days 4 and 5. RESULTS: The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0 × 104 µm(2) vs 29.4 × 104 µm(2), P < .05), but improved in the TU‐100 group (15.4 × 104 µm(2), P < .005). TU‐100 significantly induced PD‐1 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU‐100 = 4.3 ± 4.9 vs 5.1 ± 10.3 vs 17.9 ± 17.8). The CD4(+) cells were increased in the BT group (P < .05) compared to the control group but decreased in the TU‐100 group. The Foxp3(+) cells were increased in the BT group compared to the control group (P < .05), and further increased in the TU‐100 group compared to the BT group. CPT‐11 significantly increased TLR4, NF‐κβ, TNF‐α mRNA expressions in the BT group. TU‐100 cotreatment significantly reversed these mRNA expressions. CONCLUSION: TU‐100 may have a protective effect against BT through PD‐1 expression in Peyer's patch. |
format | Online Article Text |
id | pubmed-8452476 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84524762021-09-27 Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model Takasu, Chie Miyazaki, Katsuki Yoshikawa, Kozo Nishi, Masaaki Tokunaga, Takuya Kashihara, Hideya Yoshimoto, Toshiaki Ogawa, Hirohisa Morine, Yuji Shimada, Mitsuo Ann Gastroenterol Surg Original Articles BACKGROUND: Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulates the immune response during BT by inducing PD‐1 expression in Peyer's patches. METHODS: Eighteen male Wistar rats were divided into four groups: a control group; a control + TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d; a BT group, given CPT‐11 250 mg/kg intra‐peritoneal for 2 d; and a TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d with CPT‐11 250 mg/kg intra‐peritoneal on days 4 and 5. RESULTS: The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0 × 104 µm(2) vs 29.4 × 104 µm(2), P < .05), but improved in the TU‐100 group (15.4 × 104 µm(2), P < .005). TU‐100 significantly induced PD‐1 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU‐100 = 4.3 ± 4.9 vs 5.1 ± 10.3 vs 17.9 ± 17.8). The CD4(+) cells were increased in the BT group (P < .05) compared to the control group but decreased in the TU‐100 group. The Foxp3(+) cells were increased in the BT group compared to the control group (P < .05), and further increased in the TU‐100 group compared to the BT group. CPT‐11 significantly increased TLR4, NF‐κβ, TNF‐α mRNA expressions in the BT group. TU‐100 cotreatment significantly reversed these mRNA expressions. CONCLUSION: TU‐100 may have a protective effect against BT through PD‐1 expression in Peyer's patch. John Wiley and Sons Inc. 2021-04-05 /pmc/articles/PMC8452476/ /pubmed/34585053 http://dx.doi.org/10.1002/ags3.12460 Text en © 2021 The Authors. Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Takasu, Chie Miyazaki, Katsuki Yoshikawa, Kozo Nishi, Masaaki Tokunaga, Takuya Kashihara, Hideya Yoshimoto, Toshiaki Ogawa, Hirohisa Morine, Yuji Shimada, Mitsuo Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model |
title | Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model |
title_full | Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model |
title_fullStr | Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model |
title_full_unstemmed | Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model |
title_short | Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model |
title_sort | effect of tu‐100 on peyer's patches in a bacterial translocation rat model |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452476/ https://www.ncbi.nlm.nih.gov/pubmed/34585053 http://dx.doi.org/10.1002/ags3.12460 |
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