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Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model

BACKGROUND: Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulat...

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Autores principales: Takasu, Chie, Miyazaki, Katsuki, Yoshikawa, Kozo, Nishi, Masaaki, Tokunaga, Takuya, Kashihara, Hideya, Yoshimoto, Toshiaki, Ogawa, Hirohisa, Morine, Yuji, Shimada, Mitsuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452476/
https://www.ncbi.nlm.nih.gov/pubmed/34585053
http://dx.doi.org/10.1002/ags3.12460
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author Takasu, Chie
Miyazaki, Katsuki
Yoshikawa, Kozo
Nishi, Masaaki
Tokunaga, Takuya
Kashihara, Hideya
Yoshimoto, Toshiaki
Ogawa, Hirohisa
Morine, Yuji
Shimada, Mitsuo
author_facet Takasu, Chie
Miyazaki, Katsuki
Yoshikawa, Kozo
Nishi, Masaaki
Tokunaga, Takuya
Kashihara, Hideya
Yoshimoto, Toshiaki
Ogawa, Hirohisa
Morine, Yuji
Shimada, Mitsuo
author_sort Takasu, Chie
collection PubMed
description BACKGROUND: Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulates the immune response during BT by inducing PD‐1 expression in Peyer's patches. METHODS: Eighteen male Wistar rats were divided into four groups: a control group; a control + TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d; a BT group, given CPT‐11 250 mg/kg intra‐peritoneal for 2 d; and a TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d with CPT‐11 250 mg/kg intra‐peritoneal on days 4 and 5. RESULTS: The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0 × 104 µm(2) vs 29.4 × 104 µm(2), P < .05), but improved in the TU‐100 group (15.4 × 104 µm(2), P < .005). TU‐100 significantly induced PD‐1 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU‐100 = 4.3 ± 4.9 vs 5.1 ± 10.3 vs 17.9 ± 17.8). The CD4(+) cells were increased in the BT group (P < .05) compared to the control group but decreased in the TU‐100 group. The Foxp3(+) cells were increased in the BT group compared to the control group (P < .05), and further increased in the TU‐100 group compared to the BT group. CPT‐11 significantly increased TLR4, NF‐κβ, TNF‐α mRNA expressions in the BT group. TU‐100 cotreatment significantly reversed these mRNA expressions. CONCLUSION: TU‐100 may have a protective effect against BT through PD‐1 expression in Peyer's patch.
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spelling pubmed-84524762021-09-27 Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model Takasu, Chie Miyazaki, Katsuki Yoshikawa, Kozo Nishi, Masaaki Tokunaga, Takuya Kashihara, Hideya Yoshimoto, Toshiaki Ogawa, Hirohisa Morine, Yuji Shimada, Mitsuo Ann Gastroenterol Surg Original Articles BACKGROUND: Daikenchuto (TU‐100), a Japanese herbal medicine, is widely used for various gastrointestinal diseases. We have previously reported that TU‐100 suppresses CPT‐11‐induced bacterial translocation (BT) by maintaining the diversity of the microbiome. In this study we show that TU‐100 modulates the immune response during BT by inducing PD‐1 expression in Peyer's patches. METHODS: Eighteen male Wistar rats were divided into four groups: a control group; a control + TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d; a BT group, given CPT‐11 250 mg/kg intra‐peritoneal for 2 d; and a TU‐100 group, given TU‐100 1000 mg/kg orally for 5 d with CPT‐11 250 mg/kg intra‐peritoneal on days 4 and 5. RESULTS: The size of Peyer's patch was significantly bigger in the BT group compared to the control group (9.0 × 104 µm(2) vs 29.4 × 104 µm(2), P < .05), but improved in the TU‐100 group (15.4 × 104 µm(2), P < .005). TU‐100 significantly induced PD‐1 expression in Peyer's patch compared to the control group and the BT group (control vs BT vs TU‐100 = 4.3 ± 4.9 vs 5.1 ± 10.3 vs 17.9 ± 17.8). The CD4(+) cells were increased in the BT group (P < .05) compared to the control group but decreased in the TU‐100 group. The Foxp3(+) cells were increased in the BT group compared to the control group (P < .05), and further increased in the TU‐100 group compared to the BT group. CPT‐11 significantly increased TLR4, NF‐κβ, TNF‐α mRNA expressions in the BT group. TU‐100 cotreatment significantly reversed these mRNA expressions. CONCLUSION: TU‐100 may have a protective effect against BT through PD‐1 expression in Peyer's patch. John Wiley and Sons Inc. 2021-04-05 /pmc/articles/PMC8452476/ /pubmed/34585053 http://dx.doi.org/10.1002/ags3.12460 Text en © 2021 The Authors. Annals of Gastroenterological Surgery published by John Wiley & Sons Australia, Ltd on behalf of The Japanese Society of Gastroenterological Surgery https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Takasu, Chie
Miyazaki, Katsuki
Yoshikawa, Kozo
Nishi, Masaaki
Tokunaga, Takuya
Kashihara, Hideya
Yoshimoto, Toshiaki
Ogawa, Hirohisa
Morine, Yuji
Shimada, Mitsuo
Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
title Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
title_full Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
title_fullStr Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
title_full_unstemmed Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
title_short Effect of TU‐100 on Peyer's patches in a bacterial translocation rat model
title_sort effect of tu‐100 on peyer's patches in a bacterial translocation rat model
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452476/
https://www.ncbi.nlm.nih.gov/pubmed/34585053
http://dx.doi.org/10.1002/ags3.12460
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