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SPAAC Pulse-Chase: A Novel Click Chemistry-Based Method to Determine the Half-Life of Cellular Proteins

Assessing the stability and degradation of proteins is central to the study of cellular biological processes. Here, we describe a novel pulse-chase method to determine the half-life of cellular proteins that overcomes the limitations of other commonly used approaches. This method takes advantage of...

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Detalles Bibliográficos
Autores principales: Morey, Trevor M., Esmaeili, Mohammad Ali, Duennwald, Martin L., Rylett, R. Jane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452969/
https://www.ncbi.nlm.nih.gov/pubmed/34557490
http://dx.doi.org/10.3389/fcell.2021.722560