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Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice

To determine the effect of 3% boric acid solution on cutaneous infections with Candida albicans (CA) in mice and its effect on skin microflora. Female mice were divided into three groups, with 18 mice in each group. Two injection sites were randomly selected, and 0.1 mL of CA mycelium suspension was...

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Detalles Bibliográficos
Autores principales: Liu, Qing, Liu, Zhao, Zhang, Changlin, Xu, Yanyan, Li, Xiaojing, Gao, Hongqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453016/
https://www.ncbi.nlm.nih.gov/pubmed/34557169
http://dx.doi.org/10.3389/fmicb.2021.709880
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author Liu, Qing
Liu, Zhao
Zhang, Changlin
Xu, Yanyan
Li, Xiaojing
Gao, Hongqi
author_facet Liu, Qing
Liu, Zhao
Zhang, Changlin
Xu, Yanyan
Li, Xiaojing
Gao, Hongqi
author_sort Liu, Qing
collection PubMed
description To determine the effect of 3% boric acid solution on cutaneous infections with Candida albicans (CA) in mice and its effect on skin microflora. Female mice were divided into three groups, with 18 mice in each group. Two injection sites were randomly selected, and 0.1 mL of CA mycelium suspension was injected into the epidermis and dermis of the back of mice. Group N was treated with sterile water for injection (SWFI). We observed the clinical manifestations, fungal fluorescence microscopic examination and colony count. Group B were hydropathically compressed with 3% boric acid solution for 30 min every 12 h. Group M was treated with SWFI, and group N was not treated. One week later, each group was observed with naked eyes, and skin samples were collected. The effect of boric acid on skin microflora was measured using Internal Transcribed Spacer Identification (ITS) and 16S rRNA genes. There were no significant changes in group M. In group B, the degree of skin injury was alleviated, the wounds healed markedly, and the exudate amount decreased. The effective rate of group B (83%) was significantly higher than that of group M (25%) (P < 0.05). The relative average abundance of Candida (P < 0.0001) and CA (P < 0.05) in group B was significantly lower than that in group M. Compared with group M, the microbial richness of group B changed little, but the diversity decreased. The flora structure of group B was significantly different from that of group M, but like that of group N. In group B, the abundance of Proteobacteria (P < 0.001), Enterobacteriaceae (P < 0.001), and Escherichia-Shigella (P < 0.001) was significantly greater, and the abundance of Firmicutes (P < 0.001), Staphylococcaceae (P < 0.001), and Staphylococcus (P < 0.001) were significantly lower. The 3% boric acid solution significantly reduced the symptoms of skin infection with Candida albicans. It inhibited the growth of Candida albicans and CA, reduced the diversity of skin microorganisms, increased the abundance of Proteobacteria, Enterobacteriaceae, Escherichia-Shigella, and reduced the abundance of Firmicutes, Staphylococcaceae, Staphylococcus.
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spelling pubmed-84530162021-09-22 Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice Liu, Qing Liu, Zhao Zhang, Changlin Xu, Yanyan Li, Xiaojing Gao, Hongqi Front Microbiol Microbiology To determine the effect of 3% boric acid solution on cutaneous infections with Candida albicans (CA) in mice and its effect on skin microflora. Female mice were divided into three groups, with 18 mice in each group. Two injection sites were randomly selected, and 0.1 mL of CA mycelium suspension was injected into the epidermis and dermis of the back of mice. Group N was treated with sterile water for injection (SWFI). We observed the clinical manifestations, fungal fluorescence microscopic examination and colony count. Group B were hydropathically compressed with 3% boric acid solution for 30 min every 12 h. Group M was treated with SWFI, and group N was not treated. One week later, each group was observed with naked eyes, and skin samples were collected. The effect of boric acid on skin microflora was measured using Internal Transcribed Spacer Identification (ITS) and 16S rRNA genes. There were no significant changes in group M. In group B, the degree of skin injury was alleviated, the wounds healed markedly, and the exudate amount decreased. The effective rate of group B (83%) was significantly higher than that of group M (25%) (P < 0.05). The relative average abundance of Candida (P < 0.0001) and CA (P < 0.05) in group B was significantly lower than that in group M. Compared with group M, the microbial richness of group B changed little, but the diversity decreased. The flora structure of group B was significantly different from that of group M, but like that of group N. In group B, the abundance of Proteobacteria (P < 0.001), Enterobacteriaceae (P < 0.001), and Escherichia-Shigella (P < 0.001) was significantly greater, and the abundance of Firmicutes (P < 0.001), Staphylococcaceae (P < 0.001), and Staphylococcus (P < 0.001) were significantly lower. The 3% boric acid solution significantly reduced the symptoms of skin infection with Candida albicans. It inhibited the growth of Candida albicans and CA, reduced the diversity of skin microorganisms, increased the abundance of Proteobacteria, Enterobacteriaceae, Escherichia-Shigella, and reduced the abundance of Firmicutes, Staphylococcaceae, Staphylococcus. Frontiers Media S.A. 2021-09-07 /pmc/articles/PMC8453016/ /pubmed/34557169 http://dx.doi.org/10.3389/fmicb.2021.709880 Text en Copyright © 2021 Liu, Liu, Zhang, Xu, Li and Gao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Qing
Liu, Zhao
Zhang, Changlin
Xu, Yanyan
Li, Xiaojing
Gao, Hongqi
Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice
title Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice
title_full Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice
title_fullStr Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice
title_full_unstemmed Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice
title_short Effects of 3% Boric Acid Solution on Cutaneous Candida albicans Infection and Microecological Flora Mice
title_sort effects of 3% boric acid solution on cutaneous candida albicans infection and microecological flora mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453016/
https://www.ncbi.nlm.nih.gov/pubmed/34557169
http://dx.doi.org/10.3389/fmicb.2021.709880
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