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Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke
Deproteinized calf serum (DCS) may have neuroprotective effects after ischemic stroke. The aim of this study is to investigate whether and how the DCS inhibits neuronal injury following cerebral ischemia. Rats were subjected to 2 h transient middle cerebral artery occlusion (MCAO). One dose of 0.125...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453072/ https://www.ncbi.nlm.nih.gov/pubmed/34557139 http://dx.doi.org/10.3389/fneur.2021.636494 |
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author | Li, Weiwei Guo, Anchen Sun, Ming Wang, Jiachuan Wang, Qun |
author_facet | Li, Weiwei Guo, Anchen Sun, Ming Wang, Jiachuan Wang, Qun |
author_sort | Li, Weiwei |
collection | PubMed |
description | Deproteinized calf serum (DCS) may have neuroprotective effects after ischemic stroke. The aim of this study is to investigate whether and how the DCS inhibits neuronal injury following cerebral ischemia. Rats were subjected to 2 h transient middle cerebral artery occlusion (MCAO). One dose of 0.125 mg/gbw DCS was given immediately after reperfusion. Neurological deficit and infarct volume at 24 h post-MCAO in DCS-treated rats were lower than those in vehicle-treated rats (p < 0.0005). In cultured neurons model, cell viability was decreased, and apoptosis was increased by oxygen-glucose deprivation/reperfusion (OGD/R) (p < 0.0005). These effects of OGD/R were attenuated by 0.4 μg/μl DCS (p < 0.05) that were validated by CCK8 cell viability assay, phycoerythrin–Annexin V Apoptosis Detection assay, and TUNEL assay. Furthermore, the increase of intracellular ROS level in cultured neurons was suppressed by DCS (p < 0.05). Compared with cells subjected to OGD/R, the expression level of Bax protein decreased, and bcl-2 protein increased after DSC treatment (p < 0.05). Overall, the neuroprotective effects of DCS following cerebral ischemia may in part be due to decreased ROS production and inhibition of apoptosis. |
format | Online Article Text |
id | pubmed-8453072 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84530722021-09-22 Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke Li, Weiwei Guo, Anchen Sun, Ming Wang, Jiachuan Wang, Qun Front Neurol Neurology Deproteinized calf serum (DCS) may have neuroprotective effects after ischemic stroke. The aim of this study is to investigate whether and how the DCS inhibits neuronal injury following cerebral ischemia. Rats were subjected to 2 h transient middle cerebral artery occlusion (MCAO). One dose of 0.125 mg/gbw DCS was given immediately after reperfusion. Neurological deficit and infarct volume at 24 h post-MCAO in DCS-treated rats were lower than those in vehicle-treated rats (p < 0.0005). In cultured neurons model, cell viability was decreased, and apoptosis was increased by oxygen-glucose deprivation/reperfusion (OGD/R) (p < 0.0005). These effects of OGD/R were attenuated by 0.4 μg/μl DCS (p < 0.05) that were validated by CCK8 cell viability assay, phycoerythrin–Annexin V Apoptosis Detection assay, and TUNEL assay. Furthermore, the increase of intracellular ROS level in cultured neurons was suppressed by DCS (p < 0.05). Compared with cells subjected to OGD/R, the expression level of Bax protein decreased, and bcl-2 protein increased after DSC treatment (p < 0.05). Overall, the neuroprotective effects of DCS following cerebral ischemia may in part be due to decreased ROS production and inhibition of apoptosis. Frontiers Media S.A. 2021-09-07 /pmc/articles/PMC8453072/ /pubmed/34557139 http://dx.doi.org/10.3389/fneur.2021.636494 Text en Copyright © 2021 Li, Guo, Sun, Wang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neurology Li, Weiwei Guo, Anchen Sun, Ming Wang, Jiachuan Wang, Qun Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke |
title | Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke |
title_full | Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke |
title_fullStr | Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke |
title_full_unstemmed | Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke |
title_short | Neuroprotective Effects of Deproteinized Calf Serum in Ischemic Stroke |
title_sort | neuroprotective effects of deproteinized calf serum in ischemic stroke |
topic | Neurology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453072/ https://www.ncbi.nlm.nih.gov/pubmed/34557139 http://dx.doi.org/10.3389/fneur.2021.636494 |
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