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Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment
Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the hos...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453086/ https://www.ncbi.nlm.nih.gov/pubmed/34557170 http://dx.doi.org/10.3389/fmicb.2021.714994 |
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author | Lamret, Fabien Varin-Simon, Jennifer Velard, Frédéric Terryn, Christine Mongaret, Céline Colin, Marius Gangloff, Sophie C. Reffuveille, Fany |
author_facet | Lamret, Fabien Varin-Simon, Jennifer Velard, Frédéric Terryn, Christine Mongaret, Céline Colin, Marius Gangloff, Sophie C. Reffuveille, Fany |
author_sort | Lamret, Fabien |
collection | PubMed |
description | Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the host immune system responses and to potential antibiotic treatments. We suggested that the periprosthetic bone environment is stressful for bacteria, influencing biofilm development. To provide insights into S. aureus biofilm properties of three strains [including one methicillin-resistant S. aureus (MRSA)] under this specific environment, we assessed several parameters related to bone conditions and expected to affect biofilm characteristics. We reported that the three strains harbored different behaviors in response to the lack of oxygen, casamino acids and glucose starvation, and high concentration of magnesium. Each strain presented different biofilm biomass and live adherent cells proportion, or matrix production and composition. However, the three strains shared common responses in a bone-like environment: a similar production of extracellular DNA and engagement of the SOS response. This study is a step toward a better understanding of periprosthetic joint infections and highlights targets, which could be common among S. aureus strains and for future antibiofilm strategies. |
format | Online Article Text |
id | pubmed-8453086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84530862021-09-22 Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment Lamret, Fabien Varin-Simon, Jennifer Velard, Frédéric Terryn, Christine Mongaret, Céline Colin, Marius Gangloff, Sophie C. Reffuveille, Fany Front Microbiol Microbiology Staphylococcus aureus species is an important threat for hospital healthcare because of frequent colonization of indwelling medical devices such as bone and joint prostheses through biofilm formations, leading to therapeutic failure. Furthermore, bacteria within biofilm are less sensitive to the host immune system responses and to potential antibiotic treatments. We suggested that the periprosthetic bone environment is stressful for bacteria, influencing biofilm development. To provide insights into S. aureus biofilm properties of three strains [including one methicillin-resistant S. aureus (MRSA)] under this specific environment, we assessed several parameters related to bone conditions and expected to affect biofilm characteristics. We reported that the three strains harbored different behaviors in response to the lack of oxygen, casamino acids and glucose starvation, and high concentration of magnesium. Each strain presented different biofilm biomass and live adherent cells proportion, or matrix production and composition. However, the three strains shared common responses in a bone-like environment: a similar production of extracellular DNA and engagement of the SOS response. This study is a step toward a better understanding of periprosthetic joint infections and highlights targets, which could be common among S. aureus strains and for future antibiofilm strategies. Frontiers Media S.A. 2021-09-07 /pmc/articles/PMC8453086/ /pubmed/34557170 http://dx.doi.org/10.3389/fmicb.2021.714994 Text en Copyright © 2021 Lamret, Varin-Simon, Velard, Terryn, Mongaret, Colin, Gangloff and Reffuveille. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Lamret, Fabien Varin-Simon, Jennifer Velard, Frédéric Terryn, Christine Mongaret, Céline Colin, Marius Gangloff, Sophie C. Reffuveille, Fany Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment |
title | Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment |
title_full | Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment |
title_fullStr | Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment |
title_full_unstemmed | Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment |
title_short | Staphylococcus aureus Strain-Dependent Biofilm Formation in Bone-Like Environment |
title_sort | staphylococcus aureus strain-dependent biofilm formation in bone-like environment |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453086/ https://www.ncbi.nlm.nih.gov/pubmed/34557170 http://dx.doi.org/10.3389/fmicb.2021.714994 |
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