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Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies

Combinatorial biology methods offer a good solution for targeting interactions of specif ic molecules by a high-throughput screening and are widely used for drug development, diagnostics, identif ication of novel monoclonal antibodies, search for linear peptide mimetics of discontinuous epitopes for...

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Autores principales: Chikaev, A.N., Rudometov, A.P., Merkulyeva, Yu.A., Karpenko, L.I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453360/
https://www.ncbi.nlm.nih.gov/pubmed/34595378
http://dx.doi.org/10.18699/VJ21.063
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author Chikaev, A.N.
Chikaev, A.N.
Rudometov, A.P.
Merkulyeva, Yu.A.
Karpenko, L.I.
author_facet Chikaev, A.N.
Chikaev, A.N.
Rudometov, A.P.
Merkulyeva, Yu.A.
Karpenko, L.I.
author_sort Chikaev, A.N.
collection PubMed
description Combinatorial biology methods offer a good solution for targeting interactions of specif ic molecules by a high-throughput screening and are widely used for drug development, diagnostics, identif ication of novel monoclonal antibodies, search for linear peptide mimetics of discontinuous epitopes for the development of immunogens or vaccine components. Among all currently available techniques, phage display remains one of the most popular approaches. Despite being a fairly old method, phage display is still widely used for studying protein-protein, peptide-protein and DNA-protein interactions due to its relative simplicity and versatility. Phage display allows highly representative libraries of peptides, proteins or their fragments to be created. Each phage particle in a library displays peptides or proteins fused to its coat protein and simultaneously carries the DNA sequence encoding the displayed peptide/protein in its genome. The biopanning procedure allows isolation of specif ic clones for almost any target, and due to the physical link between the genotype and the phenotype of recombinant phage particles it is possible to determine the structure of selected molecules. Phage display technology continues to play an important role in HIV research. A major obstacle to the development of an effective HIV vaccine is an extensive genetic and antigenic variability of the virus. According to recent data, in order to provide protection against HIV infection, the so-called broadly neutralizing antibodies that are cross-reactive against multiple viral strains of HIV must be induced, which makes the identif ication of such antibodies a key area of HIV vaccinology. In this review, we discuss the use of phage display as a tool for identif ication of HIV-specif ic antibodies with broad neutralizing activity. We provide an outline of phage display technology, brief ly describe the design of antibody phage libraries and the affinity selection procedure, and discuss the biology of HIV-1-specif ic broadly neutralizing antibodies. Finally, we summarize the studies aimed at identif ication of broadly neutralizing antibodies using various types of phage libraries.
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spelling pubmed-84533602021-09-29 Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies Chikaev, A.N. Chikaev, A.N. Rudometov, A.P. Merkulyeva, Yu.A. Karpenko, L.I. Vavilovskii Zhurnal Genet Selektsii Review Combinatorial biology methods offer a good solution for targeting interactions of specif ic molecules by a high-throughput screening and are widely used for drug development, diagnostics, identif ication of novel monoclonal antibodies, search for linear peptide mimetics of discontinuous epitopes for the development of immunogens or vaccine components. Among all currently available techniques, phage display remains one of the most popular approaches. Despite being a fairly old method, phage display is still widely used for studying protein-protein, peptide-protein and DNA-protein interactions due to its relative simplicity and versatility. Phage display allows highly representative libraries of peptides, proteins or their fragments to be created. Each phage particle in a library displays peptides or proteins fused to its coat protein and simultaneously carries the DNA sequence encoding the displayed peptide/protein in its genome. The biopanning procedure allows isolation of specif ic clones for almost any target, and due to the physical link between the genotype and the phenotype of recombinant phage particles it is possible to determine the structure of selected molecules. Phage display technology continues to play an important role in HIV research. A major obstacle to the development of an effective HIV vaccine is an extensive genetic and antigenic variability of the virus. According to recent data, in order to provide protection against HIV infection, the so-called broadly neutralizing antibodies that are cross-reactive against multiple viral strains of HIV must be induced, which makes the identif ication of such antibodies a key area of HIV vaccinology. In this review, we discuss the use of phage display as a tool for identif ication of HIV-specif ic antibodies with broad neutralizing activity. We provide an outline of phage display technology, brief ly describe the design of antibody phage libraries and the affinity selection procedure, and discuss the biology of HIV-1-specif ic broadly neutralizing antibodies. Finally, we summarize the studies aimed at identif ication of broadly neutralizing antibodies using various types of phage libraries. The Federal Research Center Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences 2021-09 /pmc/articles/PMC8453360/ /pubmed/34595378 http://dx.doi.org/10.18699/VJ21.063 Text en Copyright © AUTHORS https://creativecommons.org/licenses/by/2.5/This work is licensed under a Creative Commons Attribution 4.0 License
spellingShingle Review
Chikaev, A.N.
Chikaev, A.N.
Rudometov, A.P.
Merkulyeva, Yu.A.
Karpenko, L.I.
Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
title Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
title_full Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
title_fullStr Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
title_full_unstemmed Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
title_short Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
title_sort phage display as a tool for identifying hiv-1 broadly neutralizing antibodies
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453360/
https://www.ncbi.nlm.nih.gov/pubmed/34595378
http://dx.doi.org/10.18699/VJ21.063
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