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Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding
A recurrent pandemic with unpredictable viral nature has implied the need for a rapid diagnostic technology to facilitate timely and appropriate countermeasures against viral infections. In this study, conductive polymer-based nanoparticles have been developed as a tool for rapid diagnosis of influe...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Tsinghua University Press
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453473/ https://www.ncbi.nlm.nih.gov/pubmed/34567436 http://dx.doi.org/10.1007/s12274-021-3772-6 |
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author | Park, Geunseon Kim, Hyun-Ouk Lim, Jong-Woo Park, Chaewon Yeom, Minjoo Song, Daesub Haam, Seungjoo |
author_facet | Park, Geunseon Kim, Hyun-Ouk Lim, Jong-Woo Park, Chaewon Yeom, Minjoo Song, Daesub Haam, Seungjoo |
author_sort | Park, Geunseon |
collection | PubMed |
description | A recurrent pandemic with unpredictable viral nature has implied the need for a rapid diagnostic technology to facilitate timely and appropriate countermeasures against viral infections. In this study, conductive polymer-based nanoparticles have been developed as a tool for rapid diagnosis of influenza A (H1N1) virus. The distinctive property of a conductive polymer that transduces stimulus to respond, enabled immediate optical signal processing for the specific recognition of H1N1 virus. Conductive poly(aniline-co-pyrrole)-encapsulated polymeric vesicles, functionalized with peptides, were fabricated for the specific recognition of H1N1 virus. The low solubility of conductive polymers was successfully improved by employing vesicles consisting of amphiphilic copolymers, facilitating the viral titer-dependent production of the optical response. The optical response of the detection system to the binding event with H1N1, a mechanical stimulation, was extensively analyzed and provided concordant information on viral titers of H1N1 virus in 15 min. The specificity toward the H1N1 virus was experimentally demonstrated via a negative optical response against the control group, H3N2. Therefore, the designed system that transduces the optical response to the target-specific binding can be a rapid tool for the diagnosis of H1N1. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material (Table S1 and Figs. S1–S8) is available in the online version of this article at 10.1007/s12274-021-3772-6. |
format | Online Article Text |
id | pubmed-8453473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Tsinghua University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-84534732021-09-21 Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding Park, Geunseon Kim, Hyun-Ouk Lim, Jong-Woo Park, Chaewon Yeom, Minjoo Song, Daesub Haam, Seungjoo Nano Res Research Article A recurrent pandemic with unpredictable viral nature has implied the need for a rapid diagnostic technology to facilitate timely and appropriate countermeasures against viral infections. In this study, conductive polymer-based nanoparticles have been developed as a tool for rapid diagnosis of influenza A (H1N1) virus. The distinctive property of a conductive polymer that transduces stimulus to respond, enabled immediate optical signal processing for the specific recognition of H1N1 virus. Conductive poly(aniline-co-pyrrole)-encapsulated polymeric vesicles, functionalized with peptides, were fabricated for the specific recognition of H1N1 virus. The low solubility of conductive polymers was successfully improved by employing vesicles consisting of amphiphilic copolymers, facilitating the viral titer-dependent production of the optical response. The optical response of the detection system to the binding event with H1N1, a mechanical stimulation, was extensively analyzed and provided concordant information on viral titers of H1N1 virus in 15 min. The specificity toward the H1N1 virus was experimentally demonstrated via a negative optical response against the control group, H3N2. Therefore, the designed system that transduces the optical response to the target-specific binding can be a rapid tool for the diagnosis of H1N1. [Image: see text] ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material (Table S1 and Figs. S1–S8) is available in the online version of this article at 10.1007/s12274-021-3772-6. Tsinghua University Press 2021-09-21 2022 /pmc/articles/PMC8453473/ /pubmed/34567436 http://dx.doi.org/10.1007/s12274-021-3772-6 Text en © Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Research Article Park, Geunseon Kim, Hyun-Ouk Lim, Jong-Woo Park, Chaewon Yeom, Minjoo Song, Daesub Haam, Seungjoo Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
title | Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
title_full | Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
title_fullStr | Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
title_full_unstemmed | Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
title_short | Rapid detection of influenza A (H1N1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
title_sort | rapid detection of influenza a (h1n1) virus by conductive polymer-based nanoparticle via optical response to virus-specific binding |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453473/ https://www.ncbi.nlm.nih.gov/pubmed/34567436 http://dx.doi.org/10.1007/s12274-021-3772-6 |
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