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Fluorescence Lifetime Imaging Microscopy (FLIM) of Intracellular Transport by Means of Doubly Labelled siRNA Architectures

For monitoring the intracellular pathway of small interfering RNA (siRNA), both strands were labelled at internal positions by two ATTO dyes as an interstrand Förster resonance energy transfer pair. siRNA double strands show red emission and a short donor lifetime as readout, whereas siRNA antisense...

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Detalles Bibliográficos
Autores principales:	Doll, Larissa, Lackner, Jens, Rönicke, Franziska, Nienhaus, Gerd Ulrich, Wagenknecht, Hans‐Achim
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Full Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453559/ https://www.ncbi.nlm.nih.gov/pubmed/34125482 http://dx.doi.org/10.1002/cbic.202100150

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