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ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors

The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial‐like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglia...

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Autores principales: Kantzer, Christina Geraldine, Parmigiani, Elena, Cerrato, Valentina, Tomiuk, Stefan, Knauel, Michail, Jungblut, Melanie, Buffo, Annalisa, Bosio, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453861/
https://www.ncbi.nlm.nih.gov/pubmed/34060113
http://dx.doi.org/10.1002/jnr.24842
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author Kantzer, Christina Geraldine
Parmigiani, Elena
Cerrato, Valentina
Tomiuk, Stefan
Knauel, Michail
Jungblut, Melanie
Buffo, Annalisa
Bosio, Andreas
author_facet Kantzer, Christina Geraldine
Parmigiani, Elena
Cerrato, Valentina
Tomiuk, Stefan
Knauel, Michail
Jungblut, Melanie
Buffo, Annalisa
Bosio, Andreas
author_sort Kantzer, Christina Geraldine
collection PubMed
description The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial‐like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglial‐like progenitors found in the prospective white matter (PWM) produces astroglia and interneurons. Characterizing these cerebellar astroglia‐like progenitors and distinguishing their developmental fates is still elusive. Here, we reveal that astrocyte cell surface antigen‐2 (ACSA‐2), lately identified as ATPase, Na+/K+ transporting, beta 2 polypeptide, is expressed by glial precursors throughout postnatal cerebellar development. In contrast to common astrocyte markers, ACSA‐2 appears on PWM cells but is absent on Bergmann glia (BG) precursors. In the adult cerebellum, ACSA‐2 is broadly expressed extending to velate astrocytes in the granular layer, white matter astrocytes, and to a lesser extent to BG. Cell transplantation and transcriptomic analysis revealed that marker staining discriminates two postnatal progenitor pools. One subset is defined by the co‐expression of ACSA‐2 and GLAST and the expression of markers typical of parenchymal astrocytes. These are PWM precursors that are exclusively gliogenic. They produce predominantly white matter and granular layer astrocytes. Another subset is constituted by GLAST positive/ACSA‐2 negative precursors that express neurogenic and BG‐like progenitor genes. This population displays multipotency and gives rise to interneurons besides all glial types, including BG. In conclusion, this work reports about ACSA‐2, a marker that in combination with GLAST enables for the discrimination and isolation of multipotent and glia‐committed progenitors, which generate different types of cerebellar astrocytes.
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spelling pubmed-84538612021-09-27 ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors Kantzer, Christina Geraldine Parmigiani, Elena Cerrato, Valentina Tomiuk, Stefan Knauel, Michail Jungblut, Melanie Buffo, Annalisa Bosio, Andreas J Neurosci Res Research Articles The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial‐like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglial‐like progenitors found in the prospective white matter (PWM) produces astroglia and interneurons. Characterizing these cerebellar astroglia‐like progenitors and distinguishing their developmental fates is still elusive. Here, we reveal that astrocyte cell surface antigen‐2 (ACSA‐2), lately identified as ATPase, Na+/K+ transporting, beta 2 polypeptide, is expressed by glial precursors throughout postnatal cerebellar development. In contrast to common astrocyte markers, ACSA‐2 appears on PWM cells but is absent on Bergmann glia (BG) precursors. In the adult cerebellum, ACSA‐2 is broadly expressed extending to velate astrocytes in the granular layer, white matter astrocytes, and to a lesser extent to BG. Cell transplantation and transcriptomic analysis revealed that marker staining discriminates two postnatal progenitor pools. One subset is defined by the co‐expression of ACSA‐2 and GLAST and the expression of markers typical of parenchymal astrocytes. These are PWM precursors that are exclusively gliogenic. They produce predominantly white matter and granular layer astrocytes. Another subset is constituted by GLAST positive/ACSA‐2 negative precursors that express neurogenic and BG‐like progenitor genes. This population displays multipotency and gives rise to interneurons besides all glial types, including BG. In conclusion, this work reports about ACSA‐2, a marker that in combination with GLAST enables for the discrimination and isolation of multipotent and glia‐committed progenitors, which generate different types of cerebellar astrocytes. John Wiley and Sons Inc. 2021-05-31 2021-09 /pmc/articles/PMC8453861/ /pubmed/34060113 http://dx.doi.org/10.1002/jnr.24842 Text en © 2021 The Authors. Journal of Neuroscience Research published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Kantzer, Christina Geraldine
Parmigiani, Elena
Cerrato, Valentina
Tomiuk, Stefan
Knauel, Michail
Jungblut, Melanie
Buffo, Annalisa
Bosio, Andreas
ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
title ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
title_full ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
title_fullStr ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
title_full_unstemmed ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
title_short ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
title_sort acsa‐2 and glast classify subpopulations of multipotent and glial‐restricted cerebellar precursors
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453861/
https://www.ncbi.nlm.nih.gov/pubmed/34060113
http://dx.doi.org/10.1002/jnr.24842
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