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ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors
The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial‐like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglia...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453861/ https://www.ncbi.nlm.nih.gov/pubmed/34060113 http://dx.doi.org/10.1002/jnr.24842 |
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author | Kantzer, Christina Geraldine Parmigiani, Elena Cerrato, Valentina Tomiuk, Stefan Knauel, Michail Jungblut, Melanie Buffo, Annalisa Bosio, Andreas |
author_facet | Kantzer, Christina Geraldine Parmigiani, Elena Cerrato, Valentina Tomiuk, Stefan Knauel, Michail Jungblut, Melanie Buffo, Annalisa Bosio, Andreas |
author_sort | Kantzer, Christina Geraldine |
collection | PubMed |
description | The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial‐like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglial‐like progenitors found in the prospective white matter (PWM) produces astroglia and interneurons. Characterizing these cerebellar astroglia‐like progenitors and distinguishing their developmental fates is still elusive. Here, we reveal that astrocyte cell surface antigen‐2 (ACSA‐2), lately identified as ATPase, Na+/K+ transporting, beta 2 polypeptide, is expressed by glial precursors throughout postnatal cerebellar development. In contrast to common astrocyte markers, ACSA‐2 appears on PWM cells but is absent on Bergmann glia (BG) precursors. In the adult cerebellum, ACSA‐2 is broadly expressed extending to velate astrocytes in the granular layer, white matter astrocytes, and to a lesser extent to BG. Cell transplantation and transcriptomic analysis revealed that marker staining discriminates two postnatal progenitor pools. One subset is defined by the co‐expression of ACSA‐2 and GLAST and the expression of markers typical of parenchymal astrocytes. These are PWM precursors that are exclusively gliogenic. They produce predominantly white matter and granular layer astrocytes. Another subset is constituted by GLAST positive/ACSA‐2 negative precursors that express neurogenic and BG‐like progenitor genes. This population displays multipotency and gives rise to interneurons besides all glial types, including BG. In conclusion, this work reports about ACSA‐2, a marker that in combination with GLAST enables for the discrimination and isolation of multipotent and glia‐committed progenitors, which generate different types of cerebellar astrocytes. |
format | Online Article Text |
id | pubmed-8453861 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84538612021-09-27 ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors Kantzer, Christina Geraldine Parmigiani, Elena Cerrato, Valentina Tomiuk, Stefan Knauel, Michail Jungblut, Melanie Buffo, Annalisa Bosio, Andreas J Neurosci Res Research Articles The formation of the cerebellum is highly coordinated to obtain its characteristic morphology and all cerebellar cell types. During mouse postnatal development, cerebellar progenitors with astroglial‐like characteristics generate mainly astrocytes and oligodendrocytes. However, a subset of astroglial‐like progenitors found in the prospective white matter (PWM) produces astroglia and interneurons. Characterizing these cerebellar astroglia‐like progenitors and distinguishing their developmental fates is still elusive. Here, we reveal that astrocyte cell surface antigen‐2 (ACSA‐2), lately identified as ATPase, Na+/K+ transporting, beta 2 polypeptide, is expressed by glial precursors throughout postnatal cerebellar development. In contrast to common astrocyte markers, ACSA‐2 appears on PWM cells but is absent on Bergmann glia (BG) precursors. In the adult cerebellum, ACSA‐2 is broadly expressed extending to velate astrocytes in the granular layer, white matter astrocytes, and to a lesser extent to BG. Cell transplantation and transcriptomic analysis revealed that marker staining discriminates two postnatal progenitor pools. One subset is defined by the co‐expression of ACSA‐2 and GLAST and the expression of markers typical of parenchymal astrocytes. These are PWM precursors that are exclusively gliogenic. They produce predominantly white matter and granular layer astrocytes. Another subset is constituted by GLAST positive/ACSA‐2 negative precursors that express neurogenic and BG‐like progenitor genes. This population displays multipotency and gives rise to interneurons besides all glial types, including BG. In conclusion, this work reports about ACSA‐2, a marker that in combination with GLAST enables for the discrimination and isolation of multipotent and glia‐committed progenitors, which generate different types of cerebellar astrocytes. John Wiley and Sons Inc. 2021-05-31 2021-09 /pmc/articles/PMC8453861/ /pubmed/34060113 http://dx.doi.org/10.1002/jnr.24842 Text en © 2021 The Authors. Journal of Neuroscience Research published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Kantzer, Christina Geraldine Parmigiani, Elena Cerrato, Valentina Tomiuk, Stefan Knauel, Michail Jungblut, Melanie Buffo, Annalisa Bosio, Andreas ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
title | ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
title_full | ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
title_fullStr | ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
title_full_unstemmed | ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
title_short | ACSA‐2 and GLAST classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
title_sort | acsa‐2 and glast classify subpopulations of multipotent and glial‐restricted cerebellar precursors |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453861/ https://www.ncbi.nlm.nih.gov/pubmed/34060113 http://dx.doi.org/10.1002/jnr.24842 |
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