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Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea

BACKGROUND: This study was conducted to analyze the genetic characteristics of 41 β-lactam-resistant Escherichia coli isolates, which are one of the common causes of environmental mastitis, isolated from the bulk tank milk of 290 dairy farms in five factories operated by three dairy companies in Kor...

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Autores principales: Jung, Hye-Ri, Kim, Koeun, Lee, Young Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454163/
https://www.ncbi.nlm.nih.gov/pubmed/34548104
http://dx.doi.org/10.1186/s13620-021-00203-4
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author Jung, Hye-Ri
Kim, Koeun
Lee, Young Ju
author_facet Jung, Hye-Ri
Kim, Koeun
Lee, Young Ju
author_sort Jung, Hye-Ri
collection PubMed
description BACKGROUND: This study was conducted to analyze the genetic characteristics of 41 β-lactam-resistant Escherichia coli isolates, which are one of the common causes of environmental mastitis, isolated from the bulk tank milk of 290 dairy farms in five factories operated by three dairy companies in Korea. RESULTS: Analysis of the phenotypic and genotypic characteristics of β-lactam-resistant E. coli isolates revealed differences between factories even within the same company. Isolates from factory A1 and C1 showed high resistance to cephalothin (76.9 and 100%, respectively), which is a first-generation cephalosporins, whereas resistance to tetracycline was showed by only the isolates from factories B1 (60.0%), C2 (66.7%), and C3 (100%). Although all the 41 β-lactam-resistant E. coli isolates were positive for bla(OXA-1), bla(TEM-1) was highly prevalent in isolates from factories C2 (100%) and C3 (100%). Among 17 isolates resistant to both β-lactams and aminoglycosides, the most common multilocus sequence type was ST399 (13isolates, 76.5%). Furthermore, 2 (11.8%) and 12 (70.6%) isolates belonged to the phylogenetic groups B2 and D, respectively, which are invasive strains that cause intestinal infections, respectively. The predominant serogroup was O15 (70.6%), which is a globally distributed extraintestinal pathogen. Interestingly, one isolate from factory A1 belonged to O157 and carried six virulence genes, simultaneously. CONCLUSIONS: Although E. coli isolates were isolated from bulk tank milk, and not the clinical mastitis samples, the presence of the phylogenetic groups B2 and D, and the serogroups O15 and O157, which harbor antimicrobial resistance genes and virulence factors, can pose a threat to public health.
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spelling pubmed-84541632021-09-21 Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea Jung, Hye-Ri Kim, Koeun Lee, Young Ju Ir Vet J Research BACKGROUND: This study was conducted to analyze the genetic characteristics of 41 β-lactam-resistant Escherichia coli isolates, which are one of the common causes of environmental mastitis, isolated from the bulk tank milk of 290 dairy farms in five factories operated by three dairy companies in Korea. RESULTS: Analysis of the phenotypic and genotypic characteristics of β-lactam-resistant E. coli isolates revealed differences between factories even within the same company. Isolates from factory A1 and C1 showed high resistance to cephalothin (76.9 and 100%, respectively), which is a first-generation cephalosporins, whereas resistance to tetracycline was showed by only the isolates from factories B1 (60.0%), C2 (66.7%), and C3 (100%). Although all the 41 β-lactam-resistant E. coli isolates were positive for bla(OXA-1), bla(TEM-1) was highly prevalent in isolates from factories C2 (100%) and C3 (100%). Among 17 isolates resistant to both β-lactams and aminoglycosides, the most common multilocus sequence type was ST399 (13isolates, 76.5%). Furthermore, 2 (11.8%) and 12 (70.6%) isolates belonged to the phylogenetic groups B2 and D, respectively, which are invasive strains that cause intestinal infections, respectively. The predominant serogroup was O15 (70.6%), which is a globally distributed extraintestinal pathogen. Interestingly, one isolate from factory A1 belonged to O157 and carried six virulence genes, simultaneously. CONCLUSIONS: Although E. coli isolates were isolated from bulk tank milk, and not the clinical mastitis samples, the presence of the phylogenetic groups B2 and D, and the serogroups O15 and O157, which harbor antimicrobial resistance genes and virulence factors, can pose a threat to public health. BioMed Central 2021-09-21 /pmc/articles/PMC8454163/ /pubmed/34548104 http://dx.doi.org/10.1186/s13620-021-00203-4 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Jung, Hye-Ri
Kim, Koeun
Lee, Young Ju
Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea
title Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea
title_full Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea
title_fullStr Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea
title_full_unstemmed Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea
title_short Comparative analysis of genetic characterization of β-lactam-resistant Escherichia coli from bulk tank milk in Korea
title_sort comparative analysis of genetic characterization of β-lactam-resistant escherichia coli from bulk tank milk in korea
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454163/
https://www.ncbi.nlm.nih.gov/pubmed/34548104
http://dx.doi.org/10.1186/s13620-021-00203-4
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