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Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause sev...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454963/ https://www.ncbi.nlm.nih.gov/pubmed/34547028 http://dx.doi.org/10.1371/journal.pone.0249617 |
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author | Srednik, Mariela E. Lantz, Kristina Hicks, Jessica A. Morningstar-Shaw, Brenda R. Mackie, Tonya A. Schlater, Linda K. |
author_facet | Srednik, Mariela E. Lantz, Kristina Hicks, Jessica A. Morningstar-Shaw, Brenda R. Mackie, Tonya A. Schlater, Linda K. |
author_sort | Srednik, Mariela E. |
collection | PubMed |
description | Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014–2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3’’)-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans. |
format | Online Article Text |
id | pubmed-8454963 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-84549632021-09-22 Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States Srednik, Mariela E. Lantz, Kristina Hicks, Jessica A. Morningstar-Shaw, Brenda R. Mackie, Tonya A. Schlater, Linda K. PLoS One Research Article Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014–2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3’’)-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans. Public Library of Science 2021-09-21 /pmc/articles/PMC8454963/ /pubmed/34547028 http://dx.doi.org/10.1371/journal.pone.0249617 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication. |
spellingShingle | Research Article Srednik, Mariela E. Lantz, Kristina Hicks, Jessica A. Morningstar-Shaw, Brenda R. Mackie, Tonya A. Schlater, Linda K. Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States |
title | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States |
title_full | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States |
title_fullStr | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States |
title_full_unstemmed | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States |
title_short | Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States |
title_sort | antimicrobial resistance and genomic characterization of salmonella dublin isolates in cattle from the united states |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454963/ https://www.ncbi.nlm.nih.gov/pubmed/34547028 http://dx.doi.org/10.1371/journal.pone.0249617 |
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