Cargando…

Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States

Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause sev...

Descripción completa

Detalles Bibliográficos
Autores principales: Srednik, Mariela E., Lantz, Kristina, Hicks, Jessica A., Morningstar-Shaw, Brenda R., Mackie, Tonya A., Schlater, Linda K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454963/
https://www.ncbi.nlm.nih.gov/pubmed/34547028
http://dx.doi.org/10.1371/journal.pone.0249617
_version_ 1784570584424775680
author Srednik, Mariela E.
Lantz, Kristina
Hicks, Jessica A.
Morningstar-Shaw, Brenda R.
Mackie, Tonya A.
Schlater, Linda K.
author_facet Srednik, Mariela E.
Lantz, Kristina
Hicks, Jessica A.
Morningstar-Shaw, Brenda R.
Mackie, Tonya A.
Schlater, Linda K.
author_sort Srednik, Mariela E.
collection PubMed
description Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014–2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3’’)-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans.
format Online
Article
Text
id pubmed-8454963
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-84549632021-09-22 Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States Srednik, Mariela E. Lantz, Kristina Hicks, Jessica A. Morningstar-Shaw, Brenda R. Mackie, Tonya A. Schlater, Linda K. PLoS One Research Article Salmonella enterica subspecies enterica serotype Dublin is a host-adapted serotype in cattle, associated with enteritis and systemic disease. The primary clinical manifestation of Salmonella Dublin infection in cattle, especially calves, is respiratory disease. While rare in humans, it can cause severe illness, including bacteremia, with hospitalization and death. In the United States, S. Dublin has become one of the most multidrug-resistant serotypes. The objective of this study was to characterize S. Dublin isolates from sick cattle by analyzing phenotypic and genotypic antimicrobial resistance (AMR) profiles, the presence of plasmids, and phylogenetic relationships. S. Dublin isolates (n = 140) were selected from submissions to the NVSL for Salmonella serotyping (2014–2017) from 21 states. Isolates were tested for susceptibility against 14 class-representative antimicrobial drugs. Resistance profiles were determined using the ABRicate with Resfinder and NCBI databases, AMRFinder and PointFinder. Plasmids were detected using ABRicate with PlasmidFinder. Phylogeny was determined using vSNP. We found 98% of the isolates were resistant to more than 4 antimicrobials. Only 1 isolate was pan-susceptible and had no predicted AMR genes. All S. Dublin isolates were susceptible to azithromycin and meropenem. They showed 96% resistance to sulfonamides, 97% to tetracyclines, 95% to aminoglycosides and 85% to beta-lactams. The most common AMR genes were: sulf2 and tetA (98.6%), aph(6)-Id (97.9%), aph(3’’)-Ib, (97.1%), floR (94.3%), and blaCMY-2 (85.7%). All quinolone resistant isolates presented mutations in gyrA. Ten plasmid types were identified among all isolates with IncA/C2, IncX1, and IncFII(S) being the most frequent. The S. Dublin isolates show low genomic genetic diversity. This study provided antimicrobial susceptibility and genomic insight into S. Dublin clinical isolates from cattle in the U.S. Further sequence analysis integrating food and human origin S. Dublin isolates may provide valuable insight on increased virulence observed in humans. Public Library of Science 2021-09-21 /pmc/articles/PMC8454963/ /pubmed/34547028 http://dx.doi.org/10.1371/journal.pone.0249617 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Srednik, Mariela E.
Lantz, Kristina
Hicks, Jessica A.
Morningstar-Shaw, Brenda R.
Mackie, Tonya A.
Schlater, Linda K.
Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
title Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
title_full Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
title_fullStr Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
title_full_unstemmed Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
title_short Antimicrobial resistance and genomic characterization of Salmonella Dublin isolates in cattle from the United States
title_sort antimicrobial resistance and genomic characterization of salmonella dublin isolates in cattle from the united states
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8454963/
https://www.ncbi.nlm.nih.gov/pubmed/34547028
http://dx.doi.org/10.1371/journal.pone.0249617
work_keys_str_mv AT srednikmarielae antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates
AT lantzkristina antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates
AT hicksjessicaa antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates
AT morningstarshawbrendar antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates
AT mackietonyaa antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates
AT schlaterlindak antimicrobialresistanceandgenomiccharacterizationofsalmonelladublinisolatesincattlefromtheunitedstates