Cargando…

Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat

Both oxidative stress (OS) and inflammation are two fundamental pathological processes of acute liver injury (ALI). The current work is to investigate the effect and possible mechanism of Uncaria rhynchophylla (UR) on thioacetamide- (TAA-) induced ALI in rats. UR (100 and 200 mg/kg) was orally admin...

Descripción completa

Detalles Bibliográficos
Autores principales: Shin, Mi-Rae, Kim, Min Ju, Lee, Jin A, Roh, Seong-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455208/
https://www.ncbi.nlm.nih.gov/pubmed/34557455
http://dx.doi.org/10.1155/2021/5581816
_version_ 1784570625883373568
author Shin, Mi-Rae
Kim, Min Ju
Lee, Jin A
Roh, Seong-Soo
author_facet Shin, Mi-Rae
Kim, Min Ju
Lee, Jin A
Roh, Seong-Soo
author_sort Shin, Mi-Rae
collection PubMed
description Both oxidative stress (OS) and inflammation are two fundamental pathological processes of acute liver injury (ALI). The current work is to investigate the effect and possible mechanism of Uncaria rhynchophylla (UR) on thioacetamide- (TAA-) induced ALI in rats. UR (100 and 200 mg/kg) was orally administrated with TAA (200 mg/kg of bodyweight, intraperitoneal injection) for 3 consecutive days. ALI was confirmed using histological examination and the factors associated with OS and liver function activity measured in serum. Moreover, expressions of inflammation and collagen-related proteins were measured by the Western blot analysis. Myeloperoxidase (MPO), which mediates OS in the ALI control group, was manifested by a significant rise compared with the normal group. UR significantly reduced AST, ALT, and ammonia levels in serum. The nuclear factor-κB (NF-κB) activation induced by TAA led to increase both inflammatory mediators and cytokines. Whereas, UR administration remarkably suppressed such an overexpression. UR supplementation improved matrix metalloproteinases (MMPs) such as MMP-1, -2, and -8. In contrast, tissue inhibitors of metalloproteinases- (TIMP-) 1 level increased significantly by UR treatment. In addition, the histopathological analysis showed that the liver tissue lesions were improved obviously by UR treatment. UR may ameliorate the effects of TAA-induced ALI in rats by suppressing both OS through MPO activation and proinflammatory factors through NF-κB activation. In conclusion, UR exhibited a potent hepatoprotective effect on ALI through the suppression of OS.
format Online
Article
Text
id pubmed-8455208
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-84552082021-09-22 Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat Shin, Mi-Rae Kim, Min Ju Lee, Jin A Roh, Seong-Soo Can J Gastroenterol Hepatol Research Article Both oxidative stress (OS) and inflammation are two fundamental pathological processes of acute liver injury (ALI). The current work is to investigate the effect and possible mechanism of Uncaria rhynchophylla (UR) on thioacetamide- (TAA-) induced ALI in rats. UR (100 and 200 mg/kg) was orally administrated with TAA (200 mg/kg of bodyweight, intraperitoneal injection) for 3 consecutive days. ALI was confirmed using histological examination and the factors associated with OS and liver function activity measured in serum. Moreover, expressions of inflammation and collagen-related proteins were measured by the Western blot analysis. Myeloperoxidase (MPO), which mediates OS in the ALI control group, was manifested by a significant rise compared with the normal group. UR significantly reduced AST, ALT, and ammonia levels in serum. The nuclear factor-κB (NF-κB) activation induced by TAA led to increase both inflammatory mediators and cytokines. Whereas, UR administration remarkably suppressed such an overexpression. UR supplementation improved matrix metalloproteinases (MMPs) such as MMP-1, -2, and -8. In contrast, tissue inhibitors of metalloproteinases- (TIMP-) 1 level increased significantly by UR treatment. In addition, the histopathological analysis showed that the liver tissue lesions were improved obviously by UR treatment. UR may ameliorate the effects of TAA-induced ALI in rats by suppressing both OS through MPO activation and proinflammatory factors through NF-κB activation. In conclusion, UR exhibited a potent hepatoprotective effect on ALI through the suppression of OS. Hindawi 2021-09-13 /pmc/articles/PMC8455208/ /pubmed/34557455 http://dx.doi.org/10.1155/2021/5581816 Text en Copyright © 2021 Mi-Rae Shin et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shin, Mi-Rae
Kim, Min Ju
Lee, Jin A
Roh, Seong-Soo
Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
title Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
title_full Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
title_fullStr Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
title_full_unstemmed Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
title_short Effect of Uncaria rhynchophylla against Thioacetamide-Induced Acute Liver Injury in Rat
title_sort effect of uncaria rhynchophylla against thioacetamide-induced acute liver injury in rat
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455208/
https://www.ncbi.nlm.nih.gov/pubmed/34557455
http://dx.doi.org/10.1155/2021/5581816
work_keys_str_mv AT shinmirae effectofuncariarhynchophyllaagainstthioacetamideinducedacuteliverinjuryinrat
AT kimminju effectofuncariarhynchophyllaagainstthioacetamideinducedacuteliverinjuryinrat
AT leejina effectofuncariarhynchophyllaagainstthioacetamideinducedacuteliverinjuryinrat
AT rohseongsoo effectofuncariarhynchophyllaagainstthioacetamideinducedacuteliverinjuryinrat