Cargando…

Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States

The environment plays a key role in the spread and persistence of antimicrobial resistance (AMR). Antimicrobials and antimicrobial resistance genes (ARG) are released into the environment from sources such as wastewater treatment plants, and animal farms. This study describes an approach guided by s...

Descripción completa

Detalles Bibliográficos
Autores principales: Bueno, Irene, Beaudoin, Amanda, Arnold, William A., Kim, Taegyu, Frankson, Lara E., LaPara, Timothy M., Kanankege, Kaushi, Wammer, Kristine H., Singer, Randall S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455696/
https://www.ncbi.nlm.nih.gov/pubmed/34548591
http://dx.doi.org/10.1038/s41598-021-98300-5
_version_ 1784570726091587584
author Bueno, Irene
Beaudoin, Amanda
Arnold, William A.
Kim, Taegyu
Frankson, Lara E.
LaPara, Timothy M.
Kanankege, Kaushi
Wammer, Kristine H.
Singer, Randall S.
author_facet Bueno, Irene
Beaudoin, Amanda
Arnold, William A.
Kim, Taegyu
Frankson, Lara E.
LaPara, Timothy M.
Kanankege, Kaushi
Wammer, Kristine H.
Singer, Randall S.
author_sort Bueno, Irene
collection PubMed
description The environment plays a key role in the spread and persistence of antimicrobial resistance (AMR). Antimicrobials and antimicrobial resistance genes (ARG) are released into the environment from sources such as wastewater treatment plants, and animal farms. This study describes an approach guided by spatial mapping to quantify and predict antimicrobials and ARG in Minnesota’s waterbodies in water and sediment at two spatial scales: macro, throughout the state, and micro, in specific waterbodies. At the macroscale, the highest concentrations across all antimicrobial classes were found near populated areas. Kernel interpolation provided an approximation of antimicrobial concentrations and ARG abundance at unsampled locations. However, there was high uncertainty in these predictions, due in part to low study power and large distances between sites. At the microscale, wastewater treatment plants had an effect on ARG abundance (sul1 and sul2 in water; bla(SHV), intl1, mexB, and sul2 in sediment), but not on antimicrobial concentrations. Results from sediment reflected a long-term history, while water reflected a more transient record of antimicrobials and ARG. This study highlights the value of using spatial analyses, different spatial scales, and sampling matrices, to design an environmental monitoring approach to advance our understanding of AMR persistence and dissemination.
format Online
Article
Text
id pubmed-8455696
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-84556962021-09-24 Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States Bueno, Irene Beaudoin, Amanda Arnold, William A. Kim, Taegyu Frankson, Lara E. LaPara, Timothy M. Kanankege, Kaushi Wammer, Kristine H. Singer, Randall S. Sci Rep Article The environment plays a key role in the spread and persistence of antimicrobial resistance (AMR). Antimicrobials and antimicrobial resistance genes (ARG) are released into the environment from sources such as wastewater treatment plants, and animal farms. This study describes an approach guided by spatial mapping to quantify and predict antimicrobials and ARG in Minnesota’s waterbodies in water and sediment at two spatial scales: macro, throughout the state, and micro, in specific waterbodies. At the macroscale, the highest concentrations across all antimicrobial classes were found near populated areas. Kernel interpolation provided an approximation of antimicrobial concentrations and ARG abundance at unsampled locations. However, there was high uncertainty in these predictions, due in part to low study power and large distances between sites. At the microscale, wastewater treatment plants had an effect on ARG abundance (sul1 and sul2 in water; bla(SHV), intl1, mexB, and sul2 in sediment), but not on antimicrobial concentrations. Results from sediment reflected a long-term history, while water reflected a more transient record of antimicrobials and ARG. This study highlights the value of using spatial analyses, different spatial scales, and sampling matrices, to design an environmental monitoring approach to advance our understanding of AMR persistence and dissemination. Nature Publishing Group UK 2021-09-21 /pmc/articles/PMC8455696/ /pubmed/34548591 http://dx.doi.org/10.1038/s41598-021-98300-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Bueno, Irene
Beaudoin, Amanda
Arnold, William A.
Kim, Taegyu
Frankson, Lara E.
LaPara, Timothy M.
Kanankege, Kaushi
Wammer, Kristine H.
Singer, Randall S.
Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States
title Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States
title_full Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States
title_fullStr Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States
title_full_unstemmed Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States
title_short Quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in Minnesota, United States
title_sort quantifying and predicting antimicrobials and antimicrobial resistance genes in waterbodies through a holistic approach: a study in minnesota, united states
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455696/
https://www.ncbi.nlm.nih.gov/pubmed/34548591
http://dx.doi.org/10.1038/s41598-021-98300-5
work_keys_str_mv AT buenoirene quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT beaudoinamanda quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT arnoldwilliama quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT kimtaegyu quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT franksonlarae quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT laparatimothym quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT kanankegekaushi quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT wammerkristineh quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates
AT singerrandalls quantifyingandpredictingantimicrobialsandantimicrobialresistancegenesinwaterbodiesthroughaholisticapproachastudyinminnesotaunitedstates