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Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens
Copper (Cu) is an important trace element with a two-sided effect on the growth performance of animals, which depends on the timing and dosage of Cu addition, etc. The purpose of this study was to determine the effects of oral copper sulfate (CuSO(4), 350 ppm) on growth performance, cecal morphology...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456085/ https://www.ncbi.nlm.nih.gov/pubmed/34566941 http://dx.doi.org/10.3389/fmicb.2021.739577 |
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author | Huang, Cheng Shi, Yan Zhou, Changming Guo, Lianying Liu, Guohui Zhuang, Yu Li, Guyue Hu, Guoliang Liu, Ping Guo, Xiaoquan |
author_facet | Huang, Cheng Shi, Yan Zhou, Changming Guo, Lianying Liu, Guohui Zhuang, Yu Li, Guyue Hu, Guoliang Liu, Ping Guo, Xiaoquan |
author_sort | Huang, Cheng |
collection | PubMed |
description | Copper (Cu) is an important trace element with a two-sided effect on the growth performance of animals, which depends on the timing and dosage of Cu addition, etc. The purpose of this study was to determine the effects of oral copper sulfate (CuSO(4), 350 ppm) on growth performance, cecal morphology, and its microflora of chickens (n = 60) after 30, 60, and 90 days. The results showed that after 90 days of copper exposure, the chickens lost weight, the cecum mucosa was detached, and vacuolation and inflammatory infiltration occurred at the base of the lamina propria. In addition, using the 16S rDNA sequencing method, we observed that copper exposure changed the richness and diversity of intestinal microorganisms. At the phylum level, Proteobacteria and Actinobacteria both significantly increased, while Bacteroidetes significantly decreased in the Cu group compared with control check (CK) group. At the genus level, the relative abundance of Rikenellaceae_RC9_gut_group decreased significantly, while Ruminococcaceae_UCG-014, Lachnoclostridium, and [Eubacterium]_coprostanoligenes_group increased significantly after copper exposure, and the change in microflora was most significant at 90 days. Moreover, the relevance of genus-level bacteria was altered. PICRUST analysis revealed potential metabolic changes associated with copper exposure, such as Staphylococcus aureus infection and metabolic disorders of nutrients. To sum up, these data show that subchronic copper exposure not only affects the growth and development of chickens but also causes the imbalance of intestinal microflora, which may further induce metabolic disorders in chickens. |
format | Online Article Text |
id | pubmed-8456085 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84560852021-09-23 Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens Huang, Cheng Shi, Yan Zhou, Changming Guo, Lianying Liu, Guohui Zhuang, Yu Li, Guyue Hu, Guoliang Liu, Ping Guo, Xiaoquan Front Microbiol Microbiology Copper (Cu) is an important trace element with a two-sided effect on the growth performance of animals, which depends on the timing and dosage of Cu addition, etc. The purpose of this study was to determine the effects of oral copper sulfate (CuSO(4), 350 ppm) on growth performance, cecal morphology, and its microflora of chickens (n = 60) after 30, 60, and 90 days. The results showed that after 90 days of copper exposure, the chickens lost weight, the cecum mucosa was detached, and vacuolation and inflammatory infiltration occurred at the base of the lamina propria. In addition, using the 16S rDNA sequencing method, we observed that copper exposure changed the richness and diversity of intestinal microorganisms. At the phylum level, Proteobacteria and Actinobacteria both significantly increased, while Bacteroidetes significantly decreased in the Cu group compared with control check (CK) group. At the genus level, the relative abundance of Rikenellaceae_RC9_gut_group decreased significantly, while Ruminococcaceae_UCG-014, Lachnoclostridium, and [Eubacterium]_coprostanoligenes_group increased significantly after copper exposure, and the change in microflora was most significant at 90 days. Moreover, the relevance of genus-level bacteria was altered. PICRUST analysis revealed potential metabolic changes associated with copper exposure, such as Staphylococcus aureus infection and metabolic disorders of nutrients. To sum up, these data show that subchronic copper exposure not only affects the growth and development of chickens but also causes the imbalance of intestinal microflora, which may further induce metabolic disorders in chickens. Frontiers Media S.A. 2021-09-08 /pmc/articles/PMC8456085/ /pubmed/34566941 http://dx.doi.org/10.3389/fmicb.2021.739577 Text en Copyright © 2021 Huang, Shi, Zhou, Guo, Liu, Zhuang, Li, Hu, Liu and Guo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Huang, Cheng Shi, Yan Zhou, Changming Guo, Lianying Liu, Guohui Zhuang, Yu Li, Guyue Hu, Guoliang Liu, Ping Guo, Xiaoquan Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens |
title | Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens |
title_full | Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens |
title_fullStr | Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens |
title_full_unstemmed | Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens |
title_short | Effects of Subchronic Copper Poisoning on Cecal Histology and Its Microflora in Chickens |
title_sort | effects of subchronic copper poisoning on cecal histology and its microflora in chickens |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456085/ https://www.ncbi.nlm.nih.gov/pubmed/34566941 http://dx.doi.org/10.3389/fmicb.2021.739577 |
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