Protocol for chemically induced murine gastric tumor model

N-Methyl-N-nitrosourea, an N-nitroso compound converted from dietary nitrite by Helicobacter pylori, causes somatic mutations in epithelial cells and induces gastric premalignancy. Here, we describe a detailed protocol for induction of gastric tumor and analysis of tumor phenotypes in mice. This mod...

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Detalles Bibliográficos
Autores principales: Li, Ke, Wang, Ao, Liu, Huijuan, Li, Baojie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456110/
https://www.ncbi.nlm.nih.gov/pubmed/34585155
http://dx.doi.org/10.1016/j.xpro.2021.100814
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author Li, Ke
Wang, Ao
Liu, Huijuan
Li, Baojie
author_facet Li, Ke
Wang, Ao
Liu, Huijuan
Li, Baojie
author_sort Li, Ke
collection PubMed
description N-Methyl-N-nitrosourea, an N-nitroso compound converted from dietary nitrite by Helicobacter pylori, causes somatic mutations in epithelial cells and induces gastric premalignancy. Here, we describe a detailed protocol for induction of gastric tumor and analysis of tumor phenotypes in mice. This model can be widely used for studying the initiation and growth of gastric cancer. For complete details on the use and execution of this protocol, please refer to Li et al. (2021).
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spelling pubmed-84561102021-09-27 Protocol for chemically induced murine gastric tumor model Li, Ke Wang, Ao Liu, Huijuan Li, Baojie STAR Protoc Protocol N-Methyl-N-nitrosourea, an N-nitroso compound converted from dietary nitrite by Helicobacter pylori, causes somatic mutations in epithelial cells and induces gastric premalignancy. Here, we describe a detailed protocol for induction of gastric tumor and analysis of tumor phenotypes in mice. This model can be widely used for studying the initiation and growth of gastric cancer. For complete details on the use and execution of this protocol, please refer to Li et al. (2021). Elsevier 2021-09-17 /pmc/articles/PMC8456110/ /pubmed/34585155 http://dx.doi.org/10.1016/j.xpro.2021.100814 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Li, Ke
Wang, Ao
Liu, Huijuan
Li, Baojie
Protocol for chemically induced murine gastric tumor model
title Protocol for chemically induced murine gastric tumor model
title_full Protocol for chemically induced murine gastric tumor model
title_fullStr Protocol for chemically induced murine gastric tumor model
title_full_unstemmed Protocol for chemically induced murine gastric tumor model
title_short Protocol for chemically induced murine gastric tumor model
title_sort protocol for chemically induced murine gastric tumor model
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456110/
https://www.ncbi.nlm.nih.gov/pubmed/34585155
http://dx.doi.org/10.1016/j.xpro.2021.100814
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