Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq

Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general trans...

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Detalles Bibliográficos
Autores principales: Rødahl, Inga, Gotley, James, Andersen, Stacey B., Yu, Meihua, Mehdi, Ahmed M., Christ, Angelika N., Hamilton-Williams, Emma E., Frazer, Ian H., Lukowski, Samuel W., Chandra, Janin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456112/
https://www.ncbi.nlm.nih.gov/pubmed/34585169
http://dx.doi.org/10.1016/j.xpro.2021.100842
Descripción
Sumario:Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).

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