Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq

Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general trans...

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Autores principales: Rødahl, Inga, Gotley, James, Andersen, Stacey B., Yu, Meihua, Mehdi, Ahmed M., Christ, Angelika N., Hamilton-Williams, Emma E., Frazer, Ian H., Lukowski, Samuel W., Chandra, Janin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456112/
https://www.ncbi.nlm.nih.gov/pubmed/34585169
http://dx.doi.org/10.1016/j.xpro.2021.100842
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author Rødahl, Inga
Gotley, James
Andersen, Stacey B.
Yu, Meihua
Mehdi, Ahmed M.
Christ, Angelika N.
Hamilton-Williams, Emma E.
Frazer, Ian H.
Lukowski, Samuel W.
Chandra, Janin
author_facet Rødahl, Inga
Gotley, James
Andersen, Stacey B.
Yu, Meihua
Mehdi, Ahmed M.
Christ, Angelika N.
Hamilton-Williams, Emma E.
Frazer, Ian H.
Lukowski, Samuel W.
Chandra, Janin
author_sort Rødahl, Inga
collection PubMed
description Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).
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spelling pubmed-84561122021-09-27 Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq Rødahl, Inga Gotley, James Andersen, Stacey B. Yu, Meihua Mehdi, Ahmed M. Christ, Angelika N. Hamilton-Williams, Emma E. Frazer, Ian H. Lukowski, Samuel W. Chandra, Janin STAR Protoc Protocol Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021). Elsevier 2021-09-17 /pmc/articles/PMC8456112/ /pubmed/34585169 http://dx.doi.org/10.1016/j.xpro.2021.100842 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rødahl, Inga
Gotley, James
Andersen, Stacey B.
Yu, Meihua
Mehdi, Ahmed M.
Christ, Angelika N.
Hamilton-Williams, Emma E.
Frazer, Ian H.
Lukowski, Samuel W.
Chandra, Janin
Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
title Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
title_full Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
title_fullStr Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
title_full_unstemmed Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
title_short Acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and CITE-seq
title_sort acquisition of murine splenic myeloid cells for protein and gene expression profiling by advanced flow cytometry and cite-seq
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456112/
https://www.ncbi.nlm.nih.gov/pubmed/34585169
http://dx.doi.org/10.1016/j.xpro.2021.100842
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