Protocol for Drosophila sleep deprivation using single-chip board

Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and...

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Detalles Bibliográficos
Autores principales: Jin, Xi, Gu, Pengyu, Han, Junhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456114/
https://www.ncbi.nlm.nih.gov/pubmed/34585161
http://dx.doi.org/10.1016/j.xpro.2021.100827
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author Jin, Xi
Gu, Pengyu
Han, Junhai
author_facet Jin, Xi
Gu, Pengyu
Han, Junhai
author_sort Jin, Xi
collection PubMed
description Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in Drosophila. This deprivation system is featured by its programming flexibility. The knowledge of electronic circuits and a certain level of programming are both required to fulfill this protocol. For complete details on the use and execution of this protocol, please refer to Jin et al. (2021).
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spelling pubmed-84561142021-09-27 Protocol for Drosophila sleep deprivation using single-chip board Jin, Xi Gu, Pengyu Han, Junhai STAR Protoc Protocol Sleep behavior is characterized by long-term quiescence and increased arousal threshold, and it is homeostatically regulated. The sleep rebound after deprivation is utilized to verify the abilities to maintain homeostasis. This protocol shows how to build a programmed mechanic oscillation system and detailed procedures to conduct sleep deprivation in Drosophila. This deprivation system is featured by its programming flexibility. The knowledge of electronic circuits and a certain level of programming are both required to fulfill this protocol. For complete details on the use and execution of this protocol, please refer to Jin et al. (2021). Elsevier 2021-09-17 /pmc/articles/PMC8456114/ /pubmed/34585161 http://dx.doi.org/10.1016/j.xpro.2021.100827 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Jin, Xi
Gu, Pengyu
Han, Junhai
Protocol for Drosophila sleep deprivation using single-chip board
title Protocol for Drosophila sleep deprivation using single-chip board
title_full Protocol for Drosophila sleep deprivation using single-chip board
title_fullStr Protocol for Drosophila sleep deprivation using single-chip board
title_full_unstemmed Protocol for Drosophila sleep deprivation using single-chip board
title_short Protocol for Drosophila sleep deprivation using single-chip board
title_sort protocol for drosophila sleep deprivation using single-chip board
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456114/
https://www.ncbi.nlm.nih.gov/pubmed/34585161
http://dx.doi.org/10.1016/j.xpro.2021.100827
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