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Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression
Generation of circulating tumor cells (CTCs), a key step in tumor metastasis, occurs during surgical tumor resection, often performed under general anesthesia. Propofol is the commonly used anesthetic, but its effects on CTCs and tumor metastasis remain largely unknown. Propofol effects are investig...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456212/ https://www.ncbi.nlm.nih.gov/pubmed/34263559 http://dx.doi.org/10.1002/advs.202102079 |
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author | Liu, Qidong Sheng, Zhihao Cheng, Chun Zheng, Hui Lanuti, Michael Liu, Rong Wang, Ping Shen, Yuan Xie, Zhongcong |
author_facet | Liu, Qidong Sheng, Zhihao Cheng, Chun Zheng, Hui Lanuti, Michael Liu, Rong Wang, Ping Shen, Yuan Xie, Zhongcong |
author_sort | Liu, Qidong |
collection | PubMed |
description | Generation of circulating tumor cells (CTCs), a key step in tumor metastasis, occurs during surgical tumor resection, often performed under general anesthesia. Propofol is the commonly used anesthetic, but its effects on CTCs and tumor metastasis remain largely unknown. Propofol effects are investigated in an experimental metastasis model by injecting tumor cells and, subsequently, low‐ or standard‐dose propofol to nude mice through tail vein. Propofol‐ or vehicle‐treated tumor cells are also injected to the mice. An in vitro tumor cell–vascular endothelial cell adhesion assay, immunofluorescence, and other methods are employed to assess how propofol affects tumor cell adhesion and extension. Propofol induces more lung tumor metastasis in mice than control. Mechanistically, propofol enhances tumor cell adhesion and extension through GABA(A)R to downregulate TRIM21 expression, leading to upregulation of Src, a protein associated with cell adhesion. These results demonstrate that propofol may promote tumor metastasis through GABA(A)R–TRIM21–Src mechanism. |
format | Online Article Text |
id | pubmed-8456212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84562122021-09-27 Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression Liu, Qidong Sheng, Zhihao Cheng, Chun Zheng, Hui Lanuti, Michael Liu, Rong Wang, Ping Shen, Yuan Xie, Zhongcong Adv Sci (Weinh) Research Articles Generation of circulating tumor cells (CTCs), a key step in tumor metastasis, occurs during surgical tumor resection, often performed under general anesthesia. Propofol is the commonly used anesthetic, but its effects on CTCs and tumor metastasis remain largely unknown. Propofol effects are investigated in an experimental metastasis model by injecting tumor cells and, subsequently, low‐ or standard‐dose propofol to nude mice through tail vein. Propofol‐ or vehicle‐treated tumor cells are also injected to the mice. An in vitro tumor cell–vascular endothelial cell adhesion assay, immunofluorescence, and other methods are employed to assess how propofol affects tumor cell adhesion and extension. Propofol induces more lung tumor metastasis in mice than control. Mechanistically, propofol enhances tumor cell adhesion and extension through GABA(A)R to downregulate TRIM21 expression, leading to upregulation of Src, a protein associated with cell adhesion. These results demonstrate that propofol may promote tumor metastasis through GABA(A)R–TRIM21–Src mechanism. John Wiley and Sons Inc. 2021-07-15 /pmc/articles/PMC8456212/ /pubmed/34263559 http://dx.doi.org/10.1002/advs.202102079 Text en © 2021 The Authors. Advanced Science published by Wiley‐VCH GmbH https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Liu, Qidong Sheng, Zhihao Cheng, Chun Zheng, Hui Lanuti, Michael Liu, Rong Wang, Ping Shen, Yuan Xie, Zhongcong Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression |
title | Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression |
title_full | Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression |
title_fullStr | Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression |
title_full_unstemmed | Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression |
title_short | Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABA(A)R‐Dependent TRIM21 Modulation of Src Expression |
title_sort | anesthetic propofol promotes tumor metastasis in lungs via gaba(a)r‐dependent trim21 modulation of src expression |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456212/ https://www.ncbi.nlm.nih.gov/pubmed/34263559 http://dx.doi.org/10.1002/advs.202102079 |
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