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Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456714/ https://www.ncbi.nlm.nih.gov/pubmed/34279218 http://dx.doi.org/10.7554/eLife.68583 |
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author | Brochet, Silvia Quinn, Andrew Mars, Ruben AT Neuschwander, Nicolas Sauer, Uwe Engel, Philipp |
author_facet | Brochet, Silvia Quinn, Andrew Mars, Ruben AT Neuschwander, Nicolas Sauer, Uwe Engel, Philipp |
author_sort | Brochet, Silvia |
collection | PubMed |
description | Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic bees and in liquid cultures in the presence of either pollen (bee diet) or simple sugars. Although the four species engaged in negative interactions, they were able to stably coexist, both in vivo and in vitro. However, coexistence was only possible in the presence of pollen, and not in simple sugars, independent of the environment. Using metatranscriptomics and metabolomics, we found that the four species utilize different pollen-derived carbohydrate substrates indicating resource partitioning as the basis of coexistence. Our results show that despite longstanding host association, gut bacterial interactions can be recapitulated in vitro providing insights about bacterial coexistence when combined with in vivo experiments. |
format | Online Article Text |
id | pubmed-8456714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-84567142021-09-23 Niche partitioning facilitates coexistence of closely related honey bee gut bacteria Brochet, Silvia Quinn, Andrew Mars, Ruben AT Neuschwander, Nicolas Sauer, Uwe Engel, Philipp eLife Ecology Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic bees and in liquid cultures in the presence of either pollen (bee diet) or simple sugars. Although the four species engaged in negative interactions, they were able to stably coexist, both in vivo and in vitro. However, coexistence was only possible in the presence of pollen, and not in simple sugars, independent of the environment. Using metatranscriptomics and metabolomics, we found that the four species utilize different pollen-derived carbohydrate substrates indicating resource partitioning as the basis of coexistence. Our results show that despite longstanding host association, gut bacterial interactions can be recapitulated in vitro providing insights about bacterial coexistence when combined with in vivo experiments. eLife Sciences Publications, Ltd 2021-07-19 /pmc/articles/PMC8456714/ /pubmed/34279218 http://dx.doi.org/10.7554/eLife.68583 Text en © 2021, Brochet et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Ecology Brochet, Silvia Quinn, Andrew Mars, Ruben AT Neuschwander, Nicolas Sauer, Uwe Engel, Philipp Niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
title | Niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
title_full | Niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
title_fullStr | Niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
title_full_unstemmed | Niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
title_short | Niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
title_sort | niche partitioning facilitates coexistence of closely related honey bee gut bacteria |
topic | Ecology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456714/ https://www.ncbi.nlm.nih.gov/pubmed/34279218 http://dx.doi.org/10.7554/eLife.68583 |
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