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Niche partitioning facilitates coexistence of closely related honey bee gut bacteria

Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic...

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Autores principales: Brochet, Silvia, Quinn, Andrew, Mars, Ruben AT, Neuschwander, Nicolas, Sauer, Uwe, Engel, Philipp
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456714/
https://www.ncbi.nlm.nih.gov/pubmed/34279218
http://dx.doi.org/10.7554/eLife.68583
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author Brochet, Silvia
Quinn, Andrew
Mars, Ruben AT
Neuschwander, Nicolas
Sauer, Uwe
Engel, Philipp
author_facet Brochet, Silvia
Quinn, Andrew
Mars, Ruben AT
Neuschwander, Nicolas
Sauer, Uwe
Engel, Philipp
author_sort Brochet, Silvia
collection PubMed
description Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic bees and in liquid cultures in the presence of either pollen (bee diet) or simple sugars. Although the four species engaged in negative interactions, they were able to stably coexist, both in vivo and in vitro. However, coexistence was only possible in the presence of pollen, and not in simple sugars, independent of the environment. Using metatranscriptomics and metabolomics, we found that the four species utilize different pollen-derived carbohydrate substrates indicating resource partitioning as the basis of coexistence. Our results show that despite longstanding host association, gut bacterial interactions can be recapitulated in vitro providing insights about bacterial coexistence when combined with in vivo experiments.
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spelling pubmed-84567142021-09-23 Niche partitioning facilitates coexistence of closely related honey bee gut bacteria Brochet, Silvia Quinn, Andrew Mars, Ruben AT Neuschwander, Nicolas Sauer, Uwe Engel, Philipp eLife Ecology Ecological processes underlying bacterial coexistence in the gut are not well understood. Here, we disentangled the effect of the host and the diet on the coexistence of four closely related Lactobacillus species colonizing the honey bee gut. We serially passaged the four species through gnotobiotic bees and in liquid cultures in the presence of either pollen (bee diet) or simple sugars. Although the four species engaged in negative interactions, they were able to stably coexist, both in vivo and in vitro. However, coexistence was only possible in the presence of pollen, and not in simple sugars, independent of the environment. Using metatranscriptomics and metabolomics, we found that the four species utilize different pollen-derived carbohydrate substrates indicating resource partitioning as the basis of coexistence. Our results show that despite longstanding host association, gut bacterial interactions can be recapitulated in vitro providing insights about bacterial coexistence when combined with in vivo experiments. eLife Sciences Publications, Ltd 2021-07-19 /pmc/articles/PMC8456714/ /pubmed/34279218 http://dx.doi.org/10.7554/eLife.68583 Text en © 2021, Brochet et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Ecology
Brochet, Silvia
Quinn, Andrew
Mars, Ruben AT
Neuschwander, Nicolas
Sauer, Uwe
Engel, Philipp
Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
title Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
title_full Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
title_fullStr Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
title_full_unstemmed Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
title_short Niche partitioning facilitates coexistence of closely related honey bee gut bacteria
title_sort niche partitioning facilitates coexistence of closely related honey bee gut bacteria
topic Ecology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456714/
https://www.ncbi.nlm.nih.gov/pubmed/34279218
http://dx.doi.org/10.7554/eLife.68583
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AT marsrubenat nichepartitioningfacilitatescoexistenceofcloselyrelatedhoneybeegutbacteria
AT neuschwandernicolas nichepartitioningfacilitatescoexistenceofcloselyrelatedhoneybeegutbacteria
AT saueruwe nichepartitioningfacilitatescoexistenceofcloselyrelatedhoneybeegutbacteria
AT engelphilipp nichepartitioningfacilitatescoexistenceofcloselyrelatedhoneybeegutbacteria