Cargando…

Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration

The lack of scar-free healing and regeneration in many adult human tissues imposes severe limitations on the recovery of function after injury. In stark contrast, salamanders can functionally repair a range of clinically relevant tissues throughout adult life. The impressive ability to regenerate wh...

Descripción completa

Detalles Bibliográficos
Autores principales: Debuque, Ryan J., Hart, Andrew J., Johnson, Gabriela H., Rosenthal, Nadia A., Godwin, James W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456783/
https://www.ncbi.nlm.nih.gov/pubmed/34568347
http://dx.doi.org/10.3389/fcell.2021.750587
_version_ 1784570937064030208
author Debuque, Ryan J.
Hart, Andrew J.
Johnson, Gabriela H.
Rosenthal, Nadia A.
Godwin, James W.
author_facet Debuque, Ryan J.
Hart, Andrew J.
Johnson, Gabriela H.
Rosenthal, Nadia A.
Godwin, James W.
author_sort Debuque, Ryan J.
collection PubMed
description The lack of scar-free healing and regeneration in many adult human tissues imposes severe limitations on the recovery of function after injury. In stark contrast, salamanders can functionally repair a range of clinically relevant tissues throughout adult life. The impressive ability to regenerate whole limbs after amputation, or regenerate following cardiac injury, is critically dependent on the recruitment of (myeloid) macrophage white blood cells to the site of injury. Amputation in the absence of macrophages results in regeneration failure and scar tissue induction. Identifying the exact hematopoietic source or reservoir of myeloid cells supporting regeneration is a necessary step in characterizing differences in macrophage phenotypes regulating scarring or regeneration across species. Mammalian wounds are dominated by splenic-derived monocytes that originate in the bone marrow and differentiate into macrophages within the wound. Unlike mammals, adult axolotls do not have functional bone marrow but instead utilize liver and spleen tissues as major sites for adult hematopoiesis. To interrogate leukocyte identity, tissue origins, and modes of recruitment, we established several transgenic axolotl hematopoietic tissue transplant models and flow cytometry protocols to study cell migration and identify the source of pro-regenerative macrophages. We identified that although bidirectional trafficking of leukocytes can occur between spleen and liver tissues, the liver is the major source of leukocytes recruited to regenerating limbs. Recruitment of leukocytes and limb regeneration occurs in the absence of the spleen, thus confirming the dependence of liver-derived myeloid cells in regeneration and that splenic maturation is dispensable for the education of pro-regenerative macrophages. This work provides an important foundation for understanding the hematopoietic origins and education of myeloid cells recruited to, and essential for, adult tissue regeneration.
format Online
Article
Text
id pubmed-8456783
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-84567832021-09-23 Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration Debuque, Ryan J. Hart, Andrew J. Johnson, Gabriela H. Rosenthal, Nadia A. Godwin, James W. Front Cell Dev Biol Cell and Developmental Biology The lack of scar-free healing and regeneration in many adult human tissues imposes severe limitations on the recovery of function after injury. In stark contrast, salamanders can functionally repair a range of clinically relevant tissues throughout adult life. The impressive ability to regenerate whole limbs after amputation, or regenerate following cardiac injury, is critically dependent on the recruitment of (myeloid) macrophage white blood cells to the site of injury. Amputation in the absence of macrophages results in regeneration failure and scar tissue induction. Identifying the exact hematopoietic source or reservoir of myeloid cells supporting regeneration is a necessary step in characterizing differences in macrophage phenotypes regulating scarring or regeneration across species. Mammalian wounds are dominated by splenic-derived monocytes that originate in the bone marrow and differentiate into macrophages within the wound. Unlike mammals, adult axolotls do not have functional bone marrow but instead utilize liver and spleen tissues as major sites for adult hematopoiesis. To interrogate leukocyte identity, tissue origins, and modes of recruitment, we established several transgenic axolotl hematopoietic tissue transplant models and flow cytometry protocols to study cell migration and identify the source of pro-regenerative macrophages. We identified that although bidirectional trafficking of leukocytes can occur between spleen and liver tissues, the liver is the major source of leukocytes recruited to regenerating limbs. Recruitment of leukocytes and limb regeneration occurs in the absence of the spleen, thus confirming the dependence of liver-derived myeloid cells in regeneration and that splenic maturation is dispensable for the education of pro-regenerative macrophages. This work provides an important foundation for understanding the hematopoietic origins and education of myeloid cells recruited to, and essential for, adult tissue regeneration. Frontiers Media S.A. 2021-09-22 /pmc/articles/PMC8456783/ /pubmed/34568347 http://dx.doi.org/10.3389/fcell.2021.750587 Text en Copyright © 2021 Debuque, Hart, Johnson, Rosenthal and Godwin. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Debuque, Ryan J.
Hart, Andrew J.
Johnson, Gabriela H.
Rosenthal, Nadia A.
Godwin, James W.
Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration
title Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration
title_full Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration
title_fullStr Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration
title_full_unstemmed Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration
title_short Identification of the Adult Hematopoietic Liver as the Primary Reservoir for the Recruitment of Pro-regenerative Macrophages Required for Salamander Limb Regeneration
title_sort identification of the adult hematopoietic liver as the primary reservoir for the recruitment of pro-regenerative macrophages required for salamander limb regeneration
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456783/
https://www.ncbi.nlm.nih.gov/pubmed/34568347
http://dx.doi.org/10.3389/fcell.2021.750587
work_keys_str_mv AT debuqueryanj identificationoftheadulthematopoieticliverastheprimaryreservoirfortherecruitmentofproregenerativemacrophagesrequiredforsalamanderlimbregeneration
AT hartandrewj identificationoftheadulthematopoieticliverastheprimaryreservoirfortherecruitmentofproregenerativemacrophagesrequiredforsalamanderlimbregeneration
AT johnsongabrielah identificationoftheadulthematopoieticliverastheprimaryreservoirfortherecruitmentofproregenerativemacrophagesrequiredforsalamanderlimbregeneration
AT rosenthalnadiaa identificationoftheadulthematopoieticliverastheprimaryreservoirfortherecruitmentofproregenerativemacrophagesrequiredforsalamanderlimbregeneration
AT godwinjamesw identificationoftheadulthematopoieticliverastheprimaryreservoirfortherecruitmentofproregenerativemacrophagesrequiredforsalamanderlimbregeneration