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A real-time fluorescence assay for CPSF73, the nuclease for pre-mRNA 3′-end processing

CPSF73 is the endonuclease that catalyzes the cleavage reaction for 3′-end processing of mRNA precursors (pre-mRNAs) in two distinct machineries, a canonical machinery for the majority of pre-mRNAs and a U7 snRNP (U7 machinery) for replication-dependent histone pre-mRNAs in animal cells. CPSF73 also...

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Detalles Bibliográficos
Autores principales: Gutierrez, Pedro A., Baughman, Kirk, Sun, Yadong, Tong, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457007/
https://www.ncbi.nlm.nih.gov/pubmed/34230059
http://dx.doi.org/10.1261/rna.078764.121