Cargando…

The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture

Previous studies have proved that dynamic culture could facilitate nutrients transport and apply mechanical stimulation to the cells within three-dimensional scaffolds, thus enhancing the differentiation of stem cells towards the osteogenic phenotype. However, the effects of macropore size on osteog...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Feng, Xiao, Dongqin, Zhang, Chengdong, Zhi, Wei, Liu, Yumei, Weng, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457200/
https://www.ncbi.nlm.nih.gov/pubmed/34567788
http://dx.doi.org/10.1093/rb/rbab050
_version_ 1784571037049946112
author Shi, Feng
Xiao, Dongqin
Zhang, Chengdong
Zhi, Wei
Liu, Yumei
Weng, Jie
author_facet Shi, Feng
Xiao, Dongqin
Zhang, Chengdong
Zhi, Wei
Liu, Yumei
Weng, Jie
author_sort Shi, Feng
collection PubMed
description Previous studies have proved that dynamic culture could facilitate nutrients transport and apply mechanical stimulation to the cells within three-dimensional scaffolds, thus enhancing the differentiation of stem cells towards the osteogenic phenotype. However, the effects of macropore size on osteogenic differentiation of stem cells under dynamic condition are still unclear. Therefore, the objective of this study was to investigate the effects of macropore size of hydroxyapatite (HAp) scaffolds on osteogenic differentiation of bone mesenchymal stem cells under static and perfusion culture conditions. In vitro cell culture results showed that cell proliferation, alkaline phosphate (ALP) activity, mRNA expression of ALP, collagen-I (Col-I), osteocalcin (OCN) and osteopontin (OPN) were enhanced when cultured under perfusion condition in comparison to static culture. Under perfusion culture condition, the ALP activity and the gene expression of ALP, Col-I, OCN and OPN were enhanced with the macropore size decreasing from 1300 to 800 µm. However, with the further decrease in macropore size from 800 to 500 µm, the osteogenic related gene expression and protein secretion were reduced. Computational fluid dynamics analysis showed that the distribution areas of medium- and high-speed flow increased with the decrease in macropore size, accompanied by the increase of the fluid shear stress within the scaffolds. These results confirm the effects of macropore size on fluid flow stimuli and cell differentiation, and also help optimize the macropore size of HAp scaffolds for bone tissue engineering.
format Online
Article
Text
id pubmed-8457200
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-84572002021-09-23 The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture Shi, Feng Xiao, Dongqin Zhang, Chengdong Zhi, Wei Liu, Yumei Weng, Jie Regen Biomater Research Article Previous studies have proved that dynamic culture could facilitate nutrients transport and apply mechanical stimulation to the cells within three-dimensional scaffolds, thus enhancing the differentiation of stem cells towards the osteogenic phenotype. However, the effects of macropore size on osteogenic differentiation of stem cells under dynamic condition are still unclear. Therefore, the objective of this study was to investigate the effects of macropore size of hydroxyapatite (HAp) scaffolds on osteogenic differentiation of bone mesenchymal stem cells under static and perfusion culture conditions. In vitro cell culture results showed that cell proliferation, alkaline phosphate (ALP) activity, mRNA expression of ALP, collagen-I (Col-I), osteocalcin (OCN) and osteopontin (OPN) were enhanced when cultured under perfusion condition in comparison to static culture. Under perfusion culture condition, the ALP activity and the gene expression of ALP, Col-I, OCN and OPN were enhanced with the macropore size decreasing from 1300 to 800 µm. However, with the further decrease in macropore size from 800 to 500 µm, the osteogenic related gene expression and protein secretion were reduced. Computational fluid dynamics analysis showed that the distribution areas of medium- and high-speed flow increased with the decrease in macropore size, accompanied by the increase of the fluid shear stress within the scaffolds. These results confirm the effects of macropore size on fluid flow stimuli and cell differentiation, and also help optimize the macropore size of HAp scaffolds for bone tissue engineering. Oxford University Press 2021-09-07 /pmc/articles/PMC8457200/ /pubmed/34567788 http://dx.doi.org/10.1093/rb/rbab050 Text en © The Author(s) 2021. Published by Oxford University Press. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Shi, Feng
Xiao, Dongqin
Zhang, Chengdong
Zhi, Wei
Liu, Yumei
Weng, Jie
The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
title The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
title_full The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
title_fullStr The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
title_full_unstemmed The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
title_short The effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
title_sort effect of macropore size of hydroxyapatite scaffold on the osteogenic differentiation of bone mesenchymal stem cells under perfusion culture
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457200/
https://www.ncbi.nlm.nih.gov/pubmed/34567788
http://dx.doi.org/10.1093/rb/rbab050
work_keys_str_mv AT shifeng theeffectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT xiaodongqin theeffectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT zhangchengdong theeffectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT zhiwei theeffectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT liuyumei theeffectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT wengjie theeffectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT shifeng effectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT xiaodongqin effectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT zhangchengdong effectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT zhiwei effectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT liuyumei effectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture
AT wengjie effectofmacroporesizeofhydroxyapatitescaffoldontheosteogenicdifferentiationofbonemesenchymalstemcellsunderperfusionculture