Cargando…
Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. In this study, we collected fecal samp...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457552/ https://www.ncbi.nlm.nih.gov/pubmed/34566901 http://dx.doi.org/10.3389/fmicb.2021.667632 |
_version_ | 1784571120249208832 |
---|---|
author | Ye, Panpan Zhang, Xueyou Xu, Yufeng Xu, Jia Song, Xiaoxiao Yao, Ke |
author_facet | Ye, Panpan Zhang, Xueyou Xu, Yufeng Xu, Jia Song, Xiaoxiao Yao, Ke |
author_sort | Ye, Panpan |
collection | PubMed |
description | Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. In this study, we collected fecal samples from 45 patients with proliferative diabetic retinopathy (PDR) and 90 matched diabetic patients (1:2 according to age, sex, and duration of diabetes) without DR (NDR) and performed 16S rRNA gene sequencing and untargeted metabolomics. We observed significantly lower bacterial diversity in the PDR group than that in the NDR group. Differential gut bacterial composition was also found, with significant depletion of 22 families (e.g., Coriobacteriaceae, Veillonellaceae, and Streptococcaceae) and enrichment of two families (Burkholderiaceae and Burkholderiales_unclassified) in the PDR group as compared with the NDR group. There were significantly different fecal metabolic features, which were enriched in metabolic pathways such as arachidonic acid and microbial metabolism, between the two groups. Among 36 coabundance metabolite clusters, 11 were positively/negatively contributed to PDR using logistic regression analysis. Fifteen gut microbial families were significantly correlated with the 11 metabolite clusters. Furthermore, a fecal metabolite-based classifier was constructed to distinguish PDR patients from NDR patients accurately. In conclusion, PDR is associated with reduced diversity and altered composition of gut microbiota and specific microbe-metabolite interplay. Our findings help to better understand the disease pathogenesis and provide novel diagnostic and therapeutic targets for PDR. |
format | Online Article Text |
id | pubmed-8457552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84575522021-09-23 Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy Ye, Panpan Zhang, Xueyou Xu, Yufeng Xu, Jia Song, Xiaoxiao Yao, Ke Front Microbiol Microbiology Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. In this study, we collected fecal samples from 45 patients with proliferative diabetic retinopathy (PDR) and 90 matched diabetic patients (1:2 according to age, sex, and duration of diabetes) without DR (NDR) and performed 16S rRNA gene sequencing and untargeted metabolomics. We observed significantly lower bacterial diversity in the PDR group than that in the NDR group. Differential gut bacterial composition was also found, with significant depletion of 22 families (e.g., Coriobacteriaceae, Veillonellaceae, and Streptococcaceae) and enrichment of two families (Burkholderiaceae and Burkholderiales_unclassified) in the PDR group as compared with the NDR group. There were significantly different fecal metabolic features, which were enriched in metabolic pathways such as arachidonic acid and microbial metabolism, between the two groups. Among 36 coabundance metabolite clusters, 11 were positively/negatively contributed to PDR using logistic regression analysis. Fifteen gut microbial families were significantly correlated with the 11 metabolite clusters. Furthermore, a fecal metabolite-based classifier was constructed to distinguish PDR patients from NDR patients accurately. In conclusion, PDR is associated with reduced diversity and altered composition of gut microbiota and specific microbe-metabolite interplay. Our findings help to better understand the disease pathogenesis and provide novel diagnostic and therapeutic targets for PDR. Frontiers Media S.A. 2021-09-08 /pmc/articles/PMC8457552/ /pubmed/34566901 http://dx.doi.org/10.3389/fmicb.2021.667632 Text en Copyright © 2021 Ye, Zhang, Xu, Xu, Song and Yao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Ye, Panpan Zhang, Xueyou Xu, Yufeng Xu, Jia Song, Xiaoxiao Yao, Ke Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy |
title | Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy |
title_full | Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy |
title_fullStr | Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy |
title_full_unstemmed | Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy |
title_short | Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy |
title_sort | alterations of the gut microbiome and metabolome in patients with proliferative diabetic retinopathy |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457552/ https://www.ncbi.nlm.nih.gov/pubmed/34566901 http://dx.doi.org/10.3389/fmicb.2021.667632 |
work_keys_str_mv | AT yepanpan alterationsofthegutmicrobiomeandmetabolomeinpatientswithproliferativediabeticretinopathy AT zhangxueyou alterationsofthegutmicrobiomeandmetabolomeinpatientswithproliferativediabeticretinopathy AT xuyufeng alterationsofthegutmicrobiomeandmetabolomeinpatientswithproliferativediabeticretinopathy AT xujia alterationsofthegutmicrobiomeandmetabolomeinpatientswithproliferativediabeticretinopathy AT songxiaoxiao alterationsofthegutmicrobiomeandmetabolomeinpatientswithproliferativediabeticretinopathy AT yaoke alterationsofthegutmicrobiomeandmetabolomeinpatientswithproliferativediabeticretinopathy |