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Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy

Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. In this study, we collected fecal samp...

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Autores principales: Ye, Panpan, Zhang, Xueyou, Xu, Yufeng, Xu, Jia, Song, Xiaoxiao, Yao, Ke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457552/
https://www.ncbi.nlm.nih.gov/pubmed/34566901
http://dx.doi.org/10.3389/fmicb.2021.667632
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author Ye, Panpan
Zhang, Xueyou
Xu, Yufeng
Xu, Jia
Song, Xiaoxiao
Yao, Ke
author_facet Ye, Panpan
Zhang, Xueyou
Xu, Yufeng
Xu, Jia
Song, Xiaoxiao
Yao, Ke
author_sort Ye, Panpan
collection PubMed
description Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. In this study, we collected fecal samples from 45 patients with proliferative diabetic retinopathy (PDR) and 90 matched diabetic patients (1:2 according to age, sex, and duration of diabetes) without DR (NDR) and performed 16S rRNA gene sequencing and untargeted metabolomics. We observed significantly lower bacterial diversity in the PDR group than that in the NDR group. Differential gut bacterial composition was also found, with significant depletion of 22 families (e.g., Coriobacteriaceae, Veillonellaceae, and Streptococcaceae) and enrichment of two families (Burkholderiaceae and Burkholderiales_unclassified) in the PDR group as compared with the NDR group. There were significantly different fecal metabolic features, which were enriched in metabolic pathways such as arachidonic acid and microbial metabolism, between the two groups. Among 36 coabundance metabolite clusters, 11 were positively/negatively contributed to PDR using logistic regression analysis. Fifteen gut microbial families were significantly correlated with the 11 metabolite clusters. Furthermore, a fecal metabolite-based classifier was constructed to distinguish PDR patients from NDR patients accurately. In conclusion, PDR is associated with reduced diversity and altered composition of gut microbiota and specific microbe-metabolite interplay. Our findings help to better understand the disease pathogenesis and provide novel diagnostic and therapeutic targets for PDR.
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spelling pubmed-84575522021-09-23 Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy Ye, Panpan Zhang, Xueyou Xu, Yufeng Xu, Jia Song, Xiaoxiao Yao, Ke Front Microbiol Microbiology Diabetic retinopathy (DR) has been reported to associate with gut microbiota alterations in murine models and thus “gut-retina-axis” has been proposed. However, the role of gut microbiome and the associated metabolism in DR patients still need to be elucidated. In this study, we collected fecal samples from 45 patients with proliferative diabetic retinopathy (PDR) and 90 matched diabetic patients (1:2 according to age, sex, and duration of diabetes) without DR (NDR) and performed 16S rRNA gene sequencing and untargeted metabolomics. We observed significantly lower bacterial diversity in the PDR group than that in the NDR group. Differential gut bacterial composition was also found, with significant depletion of 22 families (e.g., Coriobacteriaceae, Veillonellaceae, and Streptococcaceae) and enrichment of two families (Burkholderiaceae and Burkholderiales_unclassified) in the PDR group as compared with the NDR group. There were significantly different fecal metabolic features, which were enriched in metabolic pathways such as arachidonic acid and microbial metabolism, between the two groups. Among 36 coabundance metabolite clusters, 11 were positively/negatively contributed to PDR using logistic regression analysis. Fifteen gut microbial families were significantly correlated with the 11 metabolite clusters. Furthermore, a fecal metabolite-based classifier was constructed to distinguish PDR patients from NDR patients accurately. In conclusion, PDR is associated with reduced diversity and altered composition of gut microbiota and specific microbe-metabolite interplay. Our findings help to better understand the disease pathogenesis and provide novel diagnostic and therapeutic targets for PDR. Frontiers Media S.A. 2021-09-08 /pmc/articles/PMC8457552/ /pubmed/34566901 http://dx.doi.org/10.3389/fmicb.2021.667632 Text en Copyright © 2021 Ye, Zhang, Xu, Xu, Song and Yao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Ye, Panpan
Zhang, Xueyou
Xu, Yufeng
Xu, Jia
Song, Xiaoxiao
Yao, Ke
Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
title Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
title_full Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
title_fullStr Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
title_full_unstemmed Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
title_short Alterations of the Gut Microbiome and Metabolome in Patients With Proliferative Diabetic Retinopathy
title_sort alterations of the gut microbiome and metabolome in patients with proliferative diabetic retinopathy
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457552/
https://www.ncbi.nlm.nih.gov/pubmed/34566901
http://dx.doi.org/10.3389/fmicb.2021.667632
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