Cargando…

LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p

Background: Gastric cancer (GC) is a malignant tumor of digestive tract with high mortality. Elucidating the molecular mechanisms of GC and obtaining new molecular targets are particularly important for the prevention and treatment of GC. The discovery of long non-coding RNAs (lncRNAs) provides the...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Licheng, Dai, Anna, Zhang, Zao, Ning, Meiying, Han, Dong, Li, Li, Li, Zhuangzhuang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457584/
https://www.ncbi.nlm.nih.gov/pubmed/34496348
http://dx.doi.org/10.18632/aging.203403
_version_ 1784571128671371264
author Liu, Licheng
Dai, Anna
Zhang, Zao
Ning, Meiying
Han, Dong
Li, Li
Li, Zhuangzhuang
author_facet Liu, Licheng
Dai, Anna
Zhang, Zao
Ning, Meiying
Han, Dong
Li, Li
Li, Zhuangzhuang
author_sort Liu, Licheng
collection PubMed
description Background: Gastric cancer (GC) is a malignant tumor of digestive tract with high mortality. Elucidating the molecular mechanisms of GC and obtaining new molecular targets are particularly important for the prevention and treatment of GC. The discovery of long non-coding RNAs (lncRNAs) provides the possibility for further elucidating the molecular mechanisms of GC and discovering new molecular markers. Aim: Here, we aimed to explore the function and the mechanism of lncRNA PITPNA-AS1 in GC. Methods: High-throughput lncRNA microarray was used to compare the differences in expression profiles between tumor tissues and adjacent tissues, and to filtrate the differentially expressed lncRNAs in tumors. To analyze the relationship between lncRNA expression and clinicopathological parameters in GC. The apoptosis was detected by down-regulation of lncRNA. The effect of down-regulated lncRNA PITPNA-AS1 on the migration and invasion of GC cells was determined by wound healing and Transwell assays. The function of lncRNA PITPNA-AS1 on tumor growth was verified by tumor experiment in nude mice. Analysis of target interaction relationship was performed by luciferase assay. Results: The results of high throughput chip analysis identified that PITPNA-AS1 was up-regulated in GC tissues. Our data revealed that knockdown of PITPNA-AS1 was able to inhibit tumor development of GC cells. Meanwhile, PITPNA-AS1 could regulate SOX4 expression via targeting miR-92a-3p. Conclusion: Thus, we concluded that PITPNA-AS1 induced the development of GC cells by inhibiting miR-92a-3p and inducing SOX4. Our finding presents novel insights of GC, which may provide an underlying therapeutic target for GC treatment.
format Online
Article
Text
id pubmed-8457584
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Impact Journals
record_format MEDLINE/PubMed
spelling pubmed-84575842021-09-23 LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p Liu, Licheng Dai, Anna Zhang, Zao Ning, Meiying Han, Dong Li, Li Li, Zhuangzhuang Aging (Albany NY) Research Paper Background: Gastric cancer (GC) is a malignant tumor of digestive tract with high mortality. Elucidating the molecular mechanisms of GC and obtaining new molecular targets are particularly important for the prevention and treatment of GC. The discovery of long non-coding RNAs (lncRNAs) provides the possibility for further elucidating the molecular mechanisms of GC and discovering new molecular markers. Aim: Here, we aimed to explore the function and the mechanism of lncRNA PITPNA-AS1 in GC. Methods: High-throughput lncRNA microarray was used to compare the differences in expression profiles between tumor tissues and adjacent tissues, and to filtrate the differentially expressed lncRNAs in tumors. To analyze the relationship between lncRNA expression and clinicopathological parameters in GC. The apoptosis was detected by down-regulation of lncRNA. The effect of down-regulated lncRNA PITPNA-AS1 on the migration and invasion of GC cells was determined by wound healing and Transwell assays. The function of lncRNA PITPNA-AS1 on tumor growth was verified by tumor experiment in nude mice. Analysis of target interaction relationship was performed by luciferase assay. Results: The results of high throughput chip analysis identified that PITPNA-AS1 was up-regulated in GC tissues. Our data revealed that knockdown of PITPNA-AS1 was able to inhibit tumor development of GC cells. Meanwhile, PITPNA-AS1 could regulate SOX4 expression via targeting miR-92a-3p. Conclusion: Thus, we concluded that PITPNA-AS1 induced the development of GC cells by inhibiting miR-92a-3p and inducing SOX4. Our finding presents novel insights of GC, which may provide an underlying therapeutic target for GC treatment. Impact Journals 2021-09-08 /pmc/articles/PMC8457584/ /pubmed/34496348 http://dx.doi.org/10.18632/aging.203403 Text en Copyright: © 2021 Liu et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Liu, Licheng
Dai, Anna
Zhang, Zao
Ning, Meiying
Han, Dong
Li, Li
Li, Zhuangzhuang
LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p
title LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p
title_full LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p
title_fullStr LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p
title_full_unstemmed LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p
title_short LncRNA PITPNA-AS1 promotes gastric cancer by increasing SOX4 expression via inhibition of miR-92a-3p
title_sort lncrna pitpna-as1 promotes gastric cancer by increasing sox4 expression via inhibition of mir-92a-3p
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457584/
https://www.ncbi.nlm.nih.gov/pubmed/34496348
http://dx.doi.org/10.18632/aging.203403
work_keys_str_mv AT liulicheng lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p
AT daianna lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p
AT zhangzao lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p
AT ningmeiying lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p
AT handong lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p
AT lili lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p
AT lizhuangzhuang lncrnapitpnaas1promotesgastriccancerbyincreasingsox4expressionviainhibitionofmir92a3p