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The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae

Fungal adhesins (Als) or flocculins are family of cell surface proteins that mediate adhesion to diverse biotic and abiotic surfaces. A striking characteristic of Als proteins originally identified in the pathogenic Candida albicans is to form functional amyloids that mediate cis-interaction leading...

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Autores principales: Bouyx, Clara, Schiavone, Marion, Teste, Marie-Ange, Dague, Etienne, Sieczkowski, Nathalie, Julien, Anne, François, Jean Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457840/
https://www.ncbi.nlm.nih.gov/pubmed/34467855
http://dx.doi.org/10.7554/eLife.68592
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author Bouyx, Clara
Schiavone, Marion
Teste, Marie-Ange
Dague, Etienne
Sieczkowski, Nathalie
Julien, Anne
François, Jean Marie
author_facet Bouyx, Clara
Schiavone, Marion
Teste, Marie-Ange
Dague, Etienne
Sieczkowski, Nathalie
Julien, Anne
François, Jean Marie
author_sort Bouyx, Clara
collection PubMed
description Fungal adhesins (Als) or flocculins are family of cell surface proteins that mediate adhesion to diverse biotic and abiotic surfaces. A striking characteristic of Als proteins originally identified in the pathogenic Candida albicans is to form functional amyloids that mediate cis-interaction leading to the formation of adhesin nanodomains and trans-interaction between amyloid sequences of opposing cells. In this report, we show that flocculins encoded by FLO11 in Saccharomyces cerevisiae behave like adhesins in C. albicans. To do so, we show that the formation of nanodomains under an external physical force requires a threshold number of amyloid-forming sequences in the Flo11 protein. Then, using a genome editing approach, we constructed strains expressing variants of the Flo11 protein under the endogenous FLO11 promoter, leading to the demonstration that the loss of amyloid-forming sequences strongly reduces cell-cell interaction but has no effect on either plastic adherence or invasive growth in agar, both phenotypes being dependent on the N- and C-terminal ends of Flo11p. Finally, we show that the location of Flo11 is not altered either by the absence of amyloid-forming sequences or by the removal of the N- or C-terminus of the protein.
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spelling pubmed-84578402021-09-24 The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae Bouyx, Clara Schiavone, Marion Teste, Marie-Ange Dague, Etienne Sieczkowski, Nathalie Julien, Anne François, Jean Marie eLife Genetics and Genomics Fungal adhesins (Als) or flocculins are family of cell surface proteins that mediate adhesion to diverse biotic and abiotic surfaces. A striking characteristic of Als proteins originally identified in the pathogenic Candida albicans is to form functional amyloids that mediate cis-interaction leading to the formation of adhesin nanodomains and trans-interaction between amyloid sequences of opposing cells. In this report, we show that flocculins encoded by FLO11 in Saccharomyces cerevisiae behave like adhesins in C. albicans. To do so, we show that the formation of nanodomains under an external physical force requires a threshold number of amyloid-forming sequences in the Flo11 protein. Then, using a genome editing approach, we constructed strains expressing variants of the Flo11 protein under the endogenous FLO11 promoter, leading to the demonstration that the loss of amyloid-forming sequences strongly reduces cell-cell interaction but has no effect on either plastic adherence or invasive growth in agar, both phenotypes being dependent on the N- and C-terminal ends of Flo11p. Finally, we show that the location of Flo11 is not altered either by the absence of amyloid-forming sequences or by the removal of the N- or C-terminus of the protein. eLife Sciences Publications, Ltd 2021-09-01 /pmc/articles/PMC8457840/ /pubmed/34467855 http://dx.doi.org/10.7554/eLife.68592 Text en © 2021, Bouyx et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Genetics and Genomics
Bouyx, Clara
Schiavone, Marion
Teste, Marie-Ange
Dague, Etienne
Sieczkowski, Nathalie
Julien, Anne
François, Jean Marie
The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae
title The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae
title_full The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae
title_fullStr The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae
title_full_unstemmed The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae
title_short The dual role of amyloid-β-sheet sequences in the cell surface properties of FLO11-encoded flocculins in Saccharomyces cerevisiae
title_sort dual role of amyloid-β-sheet sequences in the cell surface properties of flo11-encoded flocculins in saccharomyces cerevisiae
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8457840/
https://www.ncbi.nlm.nih.gov/pubmed/34467855
http://dx.doi.org/10.7554/eLife.68592
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