Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster

We recently integrated into fly genetics a set of four selection and two counterselection markers and their corresponding drugs that can be used individually or in combination. These markers eliminate the need to visually screen progeny. Before using these markers in new genetic backgrounds, effecti...

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Autores principales: Matinyan, Nick, Gonzalez, Yezabel, Dierick, Herman A., Venken, Koen J.T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458262/
https://www.ncbi.nlm.nih.gov/pubmed/34585147
http://dx.doi.org/10.1016/j.xpro.2021.100783
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author Matinyan, Nick
Gonzalez, Yezabel
Dierick, Herman A.
Venken, Koen J.T.
author_facet Matinyan, Nick
Gonzalez, Yezabel
Dierick, Herman A.
Venken, Koen J.T.
author_sort Matinyan, Nick
collection PubMed
description We recently integrated into fly genetics a set of four selection and two counterselection markers and their corresponding drugs that can be used individually or in combination. These markers eliminate the need to visually screen progeny. Before using these markers in new genetic backgrounds, effective selection/counterselection concentrations should be established for each marker/drug combination. This protocol describes how to set up, perform, and analyze a drug titration curve to determine the effective selection/counterselection drug concentrations for their corresponding markers. For complete details on the use and execution of this protocol, please refer to Matinyan et al., 2021.
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spelling pubmed-84582622021-09-27 Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster Matinyan, Nick Gonzalez, Yezabel Dierick, Herman A. Venken, Koen J.T. STAR Protoc Protocol We recently integrated into fly genetics a set of four selection and two counterselection markers and their corresponding drugs that can be used individually or in combination. These markers eliminate the need to visually screen progeny. Before using these markers in new genetic backgrounds, effective selection/counterselection concentrations should be established for each marker/drug combination. This protocol describes how to set up, perform, and analyze a drug titration curve to determine the effective selection/counterselection drug concentrations for their corresponding markers. For complete details on the use and execution of this protocol, please refer to Matinyan et al., 2021. Elsevier 2021-09-14 /pmc/articles/PMC8458262/ /pubmed/34585147 http://dx.doi.org/10.1016/j.xpro.2021.100783 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Matinyan, Nick
Gonzalez, Yezabel
Dierick, Herman A.
Venken, Koen J.T.
Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster
title Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster
title_full Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster
title_fullStr Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster
title_full_unstemmed Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster
title_short Determining effective drug concentrations for selection and counterselection genetics in Drosophila melanogaster
title_sort determining effective drug concentrations for selection and counterselection genetics in drosophila melanogaster
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458262/
https://www.ncbi.nlm.nih.gov/pubmed/34585147
http://dx.doi.org/10.1016/j.xpro.2021.100783
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