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Isochoric supercooled preservation and revival of human cardiac microtissues

Low-temperature biopreservation and 3D tissue engineering present two differing routes towards eventual on-demand access to transplantable biologics, but recent advances in both fields present critical new opportunities for crossover between them. In this work, we demonstrate sub-zero centigrade pre...

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Autores principales: Powell-Palm, Matthew J., Charwat, Verena, Charrez, Berenice, Siemons, Brian, Healy, Kevin E., Rubinsky, Boris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458396/
https://www.ncbi.nlm.nih.gov/pubmed/34552201
http://dx.doi.org/10.1038/s42003-021-02650-9
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author Powell-Palm, Matthew J.
Charwat, Verena
Charrez, Berenice
Siemons, Brian
Healy, Kevin E.
Rubinsky, Boris
author_facet Powell-Palm, Matthew J.
Charwat, Verena
Charrez, Berenice
Siemons, Brian
Healy, Kevin E.
Rubinsky, Boris
author_sort Powell-Palm, Matthew J.
collection PubMed
description Low-temperature biopreservation and 3D tissue engineering present two differing routes towards eventual on-demand access to transplantable biologics, but recent advances in both fields present critical new opportunities for crossover between them. In this work, we demonstrate sub-zero centigrade preservation and revival of autonomously beating three-dimensional human induced pluripotent stem cell (hiPSC)-derived cardiac microtissues via isochoric supercooling, without the use of chemical cryoprotectants. We show that these tissues can cease autonomous beating during preservation and resume it after warming, that the supercooling process does not affect sarcomere structural integrity, and that the tissues maintain responsiveness to drug exposure following revival. Our work suggests both that functional three dimensional (3D) engineered tissues may provide an excellent high-content, low-risk testbed to study complex tissue biopreservation in a genetically human context, and that isochoric supercooling may provide a robust method for preserving and reviving engineered tissues themselves.
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spelling pubmed-84583962021-10-07 Isochoric supercooled preservation and revival of human cardiac microtissues Powell-Palm, Matthew J. Charwat, Verena Charrez, Berenice Siemons, Brian Healy, Kevin E. Rubinsky, Boris Commun Biol Article Low-temperature biopreservation and 3D tissue engineering present two differing routes towards eventual on-demand access to transplantable biologics, but recent advances in both fields present critical new opportunities for crossover between them. In this work, we demonstrate sub-zero centigrade preservation and revival of autonomously beating three-dimensional human induced pluripotent stem cell (hiPSC)-derived cardiac microtissues via isochoric supercooling, without the use of chemical cryoprotectants. We show that these tissues can cease autonomous beating during preservation and resume it after warming, that the supercooling process does not affect sarcomere structural integrity, and that the tissues maintain responsiveness to drug exposure following revival. Our work suggests both that functional three dimensional (3D) engineered tissues may provide an excellent high-content, low-risk testbed to study complex tissue biopreservation in a genetically human context, and that isochoric supercooling may provide a robust method for preserving and reviving engineered tissues themselves. Nature Publishing Group UK 2021-09-22 /pmc/articles/PMC8458396/ /pubmed/34552201 http://dx.doi.org/10.1038/s42003-021-02650-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Powell-Palm, Matthew J.
Charwat, Verena
Charrez, Berenice
Siemons, Brian
Healy, Kevin E.
Rubinsky, Boris
Isochoric supercooled preservation and revival of human cardiac microtissues
title Isochoric supercooled preservation and revival of human cardiac microtissues
title_full Isochoric supercooled preservation and revival of human cardiac microtissues
title_fullStr Isochoric supercooled preservation and revival of human cardiac microtissues
title_full_unstemmed Isochoric supercooled preservation and revival of human cardiac microtissues
title_short Isochoric supercooled preservation and revival of human cardiac microtissues
title_sort isochoric supercooled preservation and revival of human cardiac microtissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458396/
https://www.ncbi.nlm.nih.gov/pubmed/34552201
http://dx.doi.org/10.1038/s42003-021-02650-9
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