The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection

Lower respiratory tract infections are associated with high morbidity and mortality and significant clinical harm. Due to the limited ability of traditional pathogen detection methods, anti-infective therapy is mostly empirical. Therefore, it is difficult to adopt targeted drug therapy. In recent ye...

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Autores principales: Zheng, Yan, Qiu, Xiaojian, Wang, Ting, Zhang, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Cellular and Infection Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458627/
https://www.ncbi.nlm.nih.gov/pubmed/34568089
http://dx.doi.org/10.3389/fcimb.2021.694756
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author Zheng, Yan
Qiu, Xiaojian
Wang, Ting
Zhang, Jie
author_facet Zheng, Yan
Qiu, Xiaojian
Wang, Ting
Zhang, Jie
author_sort Zheng, Yan
collection PubMed
description Lower respiratory tract infections are associated with high morbidity and mortality and significant clinical harm. Due to the limited ability of traditional pathogen detection methods, anti-infective therapy is mostly empirical. Therefore, it is difficult to adopt targeted drug therapy. In recent years, metagenomic next-generation sequencing (mNGS) technology has provided a promising means for pathogen-specific diagnosis and updated the diagnostic strategy for lower respiratory tract infections. This article reviews the diagnostic value of mNGS for lower respiratory tract infections, the impact of different sampling methods on the detection efficiency of mNGS, and current technical difficulties in the clinical application of mNGS.
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spelling pubmed-84586272021-09-24 The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection Zheng, Yan Qiu, Xiaojian Wang, Ting Zhang, Jie Front Cell Infect Microbiol Cellular and Infection Microbiology Lower respiratory tract infections are associated with high morbidity and mortality and significant clinical harm. Due to the limited ability of traditional pathogen detection methods, anti-infective therapy is mostly empirical. Therefore, it is difficult to adopt targeted drug therapy. In recent years, metagenomic next-generation sequencing (mNGS) technology has provided a promising means for pathogen-specific diagnosis and updated the diagnostic strategy for lower respiratory tract infections. This article reviews the diagnostic value of mNGS for lower respiratory tract infections, the impact of different sampling methods on the detection efficiency of mNGS, and current technical difficulties in the clinical application of mNGS. Frontiers Media S.A. 2021-09-09 /pmc/articles/PMC8458627/ /pubmed/34568089 http://dx.doi.org/10.3389/fcimb.2021.694756 Text en Copyright © 2021 Zheng, Qiu, Wang and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zheng, Yan
Qiu, Xiaojian
Wang, Ting
Zhang, Jie
The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
title The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
title_full The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
title_fullStr The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
title_full_unstemmed The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
title_short The Diagnostic Value of Metagenomic Next–Generation Sequencing in Lower Respiratory Tract Infection
title_sort diagnostic value of metagenomic next–generation sequencing in lower respiratory tract infection
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458627/
https://www.ncbi.nlm.nih.gov/pubmed/34568089
http://dx.doi.org/10.3389/fcimb.2021.694756
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