Cargando…

The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study

BACKGROUND: Diabetes mellitus is a disease that has reached a dangerous point. Today, nearly 500 million men and women around the world live with diabetes. Gallic acid (Gal) affects diabetes. OBJECTIVE: To evaluate the effects of Gal and metformin (met) on the levels of glucose, insulin, testosteron...

Descripción completa

Detalles Bibliográficos
Autores principales: Behdarvand-Margha, Zeinab, Ahangarpour, Akram, Shahraki, Mohammadreza, Komeili, Gholamreza, Khorsandi, Layasadat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Knowledge E 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458920/
https://www.ncbi.nlm.nih.gov/pubmed/34568732
http://dx.doi.org/10.18502/ijrm.v19i8.9619
_version_ 1784571406741143552
author Behdarvand-Margha, Zeinab
Ahangarpour, Akram
Shahraki, Mohammadreza
Komeili, Gholamreza
Khorsandi, Layasadat
author_facet Behdarvand-Margha, Zeinab
Ahangarpour, Akram
Shahraki, Mohammadreza
Komeili, Gholamreza
Khorsandi, Layasadat
author_sort Behdarvand-Margha, Zeinab
collection PubMed
description BACKGROUND: Diabetes mellitus is a disease that has reached a dangerous point. Today, nearly 500 million men and women around the world live with diabetes. Gallic acid (Gal) affects diabetes. OBJECTIVE: To evaluate the effects of Gal and metformin (met) on the levels of glucose, insulin, testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), sperm count, antioxidant status, and histological changes in the testes of diabetic mice induced by methylglyoxal (MGO). MATERIALS AND METHODS: In this experimental study, 50 male adult NMRI mice, weighting 25-30 gr, aged 3-4 months were randomly divided into five equal groups (n = 10/each). (i) Control (vehicle, normal saline), (ii) MGO (600 mg/kg/d) orally for 28 days, (iii) Gal (50 mg/kg/d), (iv) MGO+Gal, and (v) MGO+met (200 mg/kg/d). Gal and met were administered orally for 21 consecutive days after the induction of diabetes. Blood samples were taken at 24 hr after the latest doses of treatment. Histological assessment of the testis was done, and the epididymis sperm count was obtained. Antioxidant indices, glucose, insulin, LH, FSH, and testosterone levels were measured. RESULTS: In the MGO group compared to the control group, insulin, glucose (p = 0.001), LH (p = 0.04) and malondialdehyde (p = 0.001) were increased. However, the level of testosterone (p = 0.001), seminiferous tubule diameters, epithelial height, sperm count, superoxide dismutase activity (p = 0.02), and testis volume (p = 0.01) were decreased. The results indicated that Gal and met ameliorated the MGO effects. CONCLUSION: These findings suggested that the animals receiving MGO became diabetic. According to the results, Gal and met can effectively prevent MGO-induced diabetes. The effect of Gal was equivalent and sometimes better than metformin.
format Online
Article
Text
id pubmed-8458920
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Knowledge E
record_format MEDLINE/PubMed
spelling pubmed-84589202021-09-24 The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study Behdarvand-Margha, Zeinab Ahangarpour, Akram Shahraki, Mohammadreza Komeili, Gholamreza Khorsandi, Layasadat Int J Reprod Biomed Original Article BACKGROUND: Diabetes mellitus is a disease that has reached a dangerous point. Today, nearly 500 million men and women around the world live with diabetes. Gallic acid (Gal) affects diabetes. OBJECTIVE: To evaluate the effects of Gal and metformin (met) on the levels of glucose, insulin, testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH), sperm count, antioxidant status, and histological changes in the testes of diabetic mice induced by methylglyoxal (MGO). MATERIALS AND METHODS: In this experimental study, 50 male adult NMRI mice, weighting 25-30 gr, aged 3-4 months were randomly divided into five equal groups (n = 10/each). (i) Control (vehicle, normal saline), (ii) MGO (600 mg/kg/d) orally for 28 days, (iii) Gal (50 mg/kg/d), (iv) MGO+Gal, and (v) MGO+met (200 mg/kg/d). Gal and met were administered orally for 21 consecutive days after the induction of diabetes. Blood samples were taken at 24 hr after the latest doses of treatment. Histological assessment of the testis was done, and the epididymis sperm count was obtained. Antioxidant indices, glucose, insulin, LH, FSH, and testosterone levels were measured. RESULTS: In the MGO group compared to the control group, insulin, glucose (p = 0.001), LH (p = 0.04) and malondialdehyde (p = 0.001) were increased. However, the level of testosterone (p = 0.001), seminiferous tubule diameters, epithelial height, sperm count, superoxide dismutase activity (p = 0.02), and testis volume (p = 0.01) were decreased. The results indicated that Gal and met ameliorated the MGO effects. CONCLUSION: These findings suggested that the animals receiving MGO became diabetic. According to the results, Gal and met can effectively prevent MGO-induced diabetes. The effect of Gal was equivalent and sometimes better than metformin. Knowledge E 2021-09-09 /pmc/articles/PMC8458920/ /pubmed/34568732 http://dx.doi.org/10.18502/ijrm.v19i8.9619 Text en Copyright © 2021 Behdarvand-Margha et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Behdarvand-Margha, Zeinab
Ahangarpour, Akram
Shahraki, Mohammadreza
Komeili, Gholamreza
Khorsandi, Layasadat
The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study
title The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study
title_full The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study
title_fullStr The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study
title_full_unstemmed The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study
title_short The effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: An experimental study
title_sort effects of gallic acid and metformin on male reproductive dysfunction in diabetic mice induced by methylglyoxal: an experimental study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458920/
https://www.ncbi.nlm.nih.gov/pubmed/34568732
http://dx.doi.org/10.18502/ijrm.v19i8.9619
work_keys_str_mv AT behdarvandmarghazeinab theeffectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT ahangarpourakram theeffectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT shahrakimohammadreza theeffectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT komeiligholamreza theeffectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT khorsandilayasadat theeffectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT behdarvandmarghazeinab effectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT ahangarpourakram effectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT shahrakimohammadreza effectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT komeiligholamreza effectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy
AT khorsandilayasadat effectsofgallicacidandmetforminonmalereproductivedysfunctionindiabeticmiceinducedbymethylglyoxalanexperimentalstudy