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GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean

Chloroplast biogenesis and development are highly complex processes requiring interactions between plastids and nuclear genomic products. Pentatricopeptide repeat (PPR) proteins play an essential role in the development of chloroplasts; however, it remains unclear how RNA editing factors influence s...

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Autores principales: Feng, Xingxing, Yang, Suxin, Zhang, Yaohua, Zhiyuan, Cheng, Tang, Kuanqiang, Li, Guang, Yu, Hui, Leng, Jiantian, Wang, Qingyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458969/
https://www.ncbi.nlm.nih.gov/pubmed/34567023
http://dx.doi.org/10.3389/fpls.2021.690973
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author Feng, Xingxing
Yang, Suxin
Zhang, Yaohua
Zhiyuan, Cheng
Tang, Kuanqiang
Li, Guang
Yu, Hui
Leng, Jiantian
Wang, Qingyu
author_facet Feng, Xingxing
Yang, Suxin
Zhang, Yaohua
Zhiyuan, Cheng
Tang, Kuanqiang
Li, Guang
Yu, Hui
Leng, Jiantian
Wang, Qingyu
author_sort Feng, Xingxing
collection PubMed
description Chloroplast biogenesis and development are highly complex processes requiring interactions between plastids and nuclear genomic products. Pentatricopeptide repeat (PPR) proteins play an essential role in the development of chloroplasts; however, it remains unclear how RNA editing factors influence soybean development. In this study, a Glycine max pale green leaf 2 mutant (Gmpgl2) was identified with decreased chlorophyll contents. Genetic mapping revealed that a single-nucleotide deletion at position 1949 bp in the Glyma.05g132700 gene in the Gmpgl2 mutant, resulting in a truncated GmPGL2 protein. The nuclear-encoded GmPGL2 is a PLS-type PPR protein that localizes to the chloroplasts. The C-to-U editing efficiencies of rps16, rps18, ndhB, ndhD, ndhE, and ndhF were reduced in the Gmpgl2 mutant. RNA electrophoresis mobility shift assay (REMSA) analysis further revealed that GmPGL2 binds to the immediate upstream sequences at RNA editing sites of rps16 and ndhB in vitro, respectively. In addition, GmPGL2 was found to interact with GmMORF8, GmMORF9, and GmORRM6. These results suggest that GmPGL2 participates in C-to-U RNA editing via the formation of a complex RNA editosome in soybean chloroplasts.
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spelling pubmed-84589692021-09-24 GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean Feng, Xingxing Yang, Suxin Zhang, Yaohua Zhiyuan, Cheng Tang, Kuanqiang Li, Guang Yu, Hui Leng, Jiantian Wang, Qingyu Front Plant Sci Plant Science Chloroplast biogenesis and development are highly complex processes requiring interactions between plastids and nuclear genomic products. Pentatricopeptide repeat (PPR) proteins play an essential role in the development of chloroplasts; however, it remains unclear how RNA editing factors influence soybean development. In this study, a Glycine max pale green leaf 2 mutant (Gmpgl2) was identified with decreased chlorophyll contents. Genetic mapping revealed that a single-nucleotide deletion at position 1949 bp in the Glyma.05g132700 gene in the Gmpgl2 mutant, resulting in a truncated GmPGL2 protein. The nuclear-encoded GmPGL2 is a PLS-type PPR protein that localizes to the chloroplasts. The C-to-U editing efficiencies of rps16, rps18, ndhB, ndhD, ndhE, and ndhF were reduced in the Gmpgl2 mutant. RNA electrophoresis mobility shift assay (REMSA) analysis further revealed that GmPGL2 binds to the immediate upstream sequences at RNA editing sites of rps16 and ndhB in vitro, respectively. In addition, GmPGL2 was found to interact with GmMORF8, GmMORF9, and GmORRM6. These results suggest that GmPGL2 participates in C-to-U RNA editing via the formation of a complex RNA editosome in soybean chloroplasts. Frontiers Media S.A. 2021-09-09 /pmc/articles/PMC8458969/ /pubmed/34567023 http://dx.doi.org/10.3389/fpls.2021.690973 Text en Copyright © 2021 Feng, Yang, Zhang, Zhiyuan, Tang, Li, Yu, Leng and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Feng, Xingxing
Yang, Suxin
Zhang, Yaohua
Zhiyuan, Cheng
Tang, Kuanqiang
Li, Guang
Yu, Hui
Leng, Jiantian
Wang, Qingyu
GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean
title GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean
title_full GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean
title_fullStr GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean
title_full_unstemmed GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean
title_short GmPGL2, Encoding a Pentatricopeptide Repeat Protein, Is Essential for Chloroplast RNA Editing and Biogenesis in Soybean
title_sort gmpgl2, encoding a pentatricopeptide repeat protein, is essential for chloroplast rna editing and biogenesis in soybean
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458969/
https://www.ncbi.nlm.nih.gov/pubmed/34567023
http://dx.doi.org/10.3389/fpls.2021.690973
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