Unveiling the K(+)-sensitivity of cell metabolism using genetically encoded, FRET-based K(+), glucose, and ATP biosensors

Investigating dynamic changes of mitochondrial ATP and cytosolic glucose levels of single living cells over time by genetically encoded biosensors provides an informative readout of their metabolic activities. Here, we describe how to monitor the metabolic K(+)-sensitivity of HEK293 cells exploiting...

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Detalles Bibliográficos
Autores principales: Bischof, Helmut, Burgstaller, Sandra, Graier, Wolfgang F., Lukowski, Robert, Malli, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458979/
https://www.ncbi.nlm.nih.gov/pubmed/34589717
http://dx.doi.org/10.1016/j.xpro.2021.100843

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458979/
https://www.ncbi.nlm.nih.gov/pubmed/34589717
http://dx.doi.org/10.1016/j.xpro.2021.100843

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