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Unveiling the K(+)-sensitivity of cell metabolism using genetically encoded, FRET-based K(+), glucose, and ATP biosensors
Investigating dynamic changes of mitochondrial ATP and cytosolic glucose levels of single living cells over time by genetically encoded biosensors provides an informative readout of their metabolic activities. Here, we describe how to monitor the metabolic K(+)-sensitivity of HEK293 cells exploiting...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458979/ https://www.ncbi.nlm.nih.gov/pubmed/34589717 http://dx.doi.org/10.1016/j.xpro.2021.100843 |