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Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment

The physiological condition of plants is significantly affected by viral infections. Viral proliferation occurs at the expense of the energy and protein stores in infected plant cells. At the same time, plants invest much of their remaining resources in the fight against infection, making them even...

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Autores principales: Balassa, Kinga, Balassa, György, Gondor, Orsolya Kinga, Janda, Tibor, Almási, Asztéria, Rudnóy, Szabolcs
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459037/
https://www.ncbi.nlm.nih.gov/pubmed/34588867
http://dx.doi.org/10.1016/j.sjbs.2021.05.073
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author Balassa, Kinga
Balassa, György
Gondor, Orsolya Kinga
Janda, Tibor
Almási, Asztéria
Rudnóy, Szabolcs
author_facet Balassa, Kinga
Balassa, György
Gondor, Orsolya Kinga
Janda, Tibor
Almási, Asztéria
Rudnóy, Szabolcs
author_sort Balassa, Kinga
collection PubMed
description The physiological condition of plants is significantly affected by viral infections. Viral proliferation occurs at the expense of the energy and protein stores in infected plant cells. At the same time, plants invest much of their remaining resources in the fight against infection, making them even less capable of normal growth processes. Thus, the slowdown in the development and growth processes of plants leads to a large-scale decrease in plant biomass and yields, which may be a perceptible problem even at the level of the national economy. One form of protection against viral infections is treatment with small interfering RNA (siRNA) molecules, which can directly reduce the amount of virus that multiplies in plant cells by enhancing the process of highly conserved RNA interference in plants. The present work demonstrated how pre-treatment with siRNA may provide protection against MDMV (Maize dwarf mosaic virus) infection in sweet corn (Zea mays cv. saccharata var. Honey Koern). In addition to monitoring the physiological condition of the maize plants, the accumulation of the virus in young leaves was examined, parallel, with changes in the plant RNA interference system and the ethylene (ET) biosynthetic pathway. The siRNA pre-treatment activated the plant antiviral defence system, thus significantly reducing viral RNA and coat protein levels in the youngest leaves of the plants. The lower initial amount of virus meant a weaker stress load, which allowed the plants to devote more energy to their growth and development. In contrast, small RNA pre-treatment did not initially have a significant effect on the ET biosynthetic pathway, but later a significant decrease was observed both in the level of transcription of genes responsible for ET production and, in the amount of ACC (1-aminocyclopropane-1-carboxylic acid) metabolite. The significantly better physiological condition, enhanced RNAi response and lower quantity of virus particles in siRNA pretreated plants, suggested that siRNA pre-treatment stimulated the antiviral defence mechanisms in MDMV infected plants. In addition, the consistently lower ACC content of the plants pre-treated with siRNA suggest that ET does not significantly contribute to the successful defence in this maize hybrid type against MDMV.
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spelling pubmed-84590372021-09-28 Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment Balassa, Kinga Balassa, György Gondor, Orsolya Kinga Janda, Tibor Almási, Asztéria Rudnóy, Szabolcs Saudi J Biol Sci Original Article The physiological condition of plants is significantly affected by viral infections. Viral proliferation occurs at the expense of the energy and protein stores in infected plant cells. At the same time, plants invest much of their remaining resources in the fight against infection, making them even less capable of normal growth processes. Thus, the slowdown in the development and growth processes of plants leads to a large-scale decrease in plant biomass and yields, which may be a perceptible problem even at the level of the national economy. One form of protection against viral infections is treatment with small interfering RNA (siRNA) molecules, which can directly reduce the amount of virus that multiplies in plant cells by enhancing the process of highly conserved RNA interference in plants. The present work demonstrated how pre-treatment with siRNA may provide protection against MDMV (Maize dwarf mosaic virus) infection in sweet corn (Zea mays cv. saccharata var. Honey Koern). In addition to monitoring the physiological condition of the maize plants, the accumulation of the virus in young leaves was examined, parallel, with changes in the plant RNA interference system and the ethylene (ET) biosynthetic pathway. The siRNA pre-treatment activated the plant antiviral defence system, thus significantly reducing viral RNA and coat protein levels in the youngest leaves of the plants. The lower initial amount of virus meant a weaker stress load, which allowed the plants to devote more energy to their growth and development. In contrast, small RNA pre-treatment did not initially have a significant effect on the ET biosynthetic pathway, but later a significant decrease was observed both in the level of transcription of genes responsible for ET production and, in the amount of ACC (1-aminocyclopropane-1-carboxylic acid) metabolite. The significantly better physiological condition, enhanced RNAi response and lower quantity of virus particles in siRNA pretreated plants, suggested that siRNA pre-treatment stimulated the antiviral defence mechanisms in MDMV infected plants. In addition, the consistently lower ACC content of the plants pre-treated with siRNA suggest that ET does not significantly contribute to the successful defence in this maize hybrid type against MDMV. Elsevier 2021-10 2021-06-02 /pmc/articles/PMC8459037/ /pubmed/34588867 http://dx.doi.org/10.1016/j.sjbs.2021.05.073 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Balassa, Kinga
Balassa, György
Gondor, Orsolya Kinga
Janda, Tibor
Almási, Asztéria
Rudnóy, Szabolcs
Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment
title Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment
title_full Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment
title_fullStr Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment
title_full_unstemmed Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment
title_short Changes in physiology, gene expression and ethylene biosynthesis in MDMV-infected sweet corn primed by small RNA pre-treatment
title_sort changes in physiology, gene expression and ethylene biosynthesis in mdmv-infected sweet corn primed by small rna pre-treatment
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459037/
https://www.ncbi.nlm.nih.gov/pubmed/34588867
http://dx.doi.org/10.1016/j.sjbs.2021.05.073
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