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Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis

In the present study, an effort has been made to elucidate the gut bacterial diversity of twelve species of the family Araneidae under three subfamilies collected from 5 states of India along with their predicted metabolic role in functional metabolism. Further, we also compared the host species phy...

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Detalles Bibliográficos
Autores principales: Tyagi, Kaomud, Tyagi, Inderjeet, Kumar, Vikas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459122/
https://www.ncbi.nlm.nih.gov/pubmed/34588907
http://dx.doi.org/10.1016/j.sjbs.2021.06.059
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author Tyagi, Kaomud
Tyagi, Inderjeet
Kumar, Vikas
author_facet Tyagi, Kaomud
Tyagi, Inderjeet
Kumar, Vikas
author_sort Tyagi, Kaomud
collection PubMed
description In the present study, an effort has been made to elucidate the gut bacterial diversity of twelve species of the family Araneidae under three subfamilies collected from 5 states of India along with their predicted metabolic role in functional metabolism. Further, we also compared the host species phylogeny based on partial cytochrome c oxidase subunit I (COI) sequences with the gut bacteria composition dendrogram to decipher the phylosymbiotic relationships. Analysis revealed the presence of 22 bacterial phyla, 145 families, and 364 genera in the gut, with Proteobacteria, Firmicutes, Actinobacteria, and Deinococcus-Thermus as the highest abundant phyla. Moreover, phylum Bacteriodetes was dominated only in Cyclosa mulmeinensis and Chlamydiae in Neoscona bengalensis. At the genus level, Bacillus, Acinetobacter, Cutibacterium, Pseudomonas, and Staphylococcus were the most dominant genera. Furthermore, the genus Prevotella was observed only in Cyclosa mulmeinensis, and endosymbiont Wolbachia only in Eriovixia laglaizei. The differential abundance analysis (DeSeq2) revealed the 19 significant ASVs represented by the genera like Acinetobacter, Vagoccoccus, Prevotella, Staphylococcus, Curvibacter, Corynebacterium, Paracoccus, Streptococcus, Microbacterium, and Pseudocitrobacter. The inter- and intra-subfamilies comparison based on diversity indices (alpha and beta diversity) revealed that the subfamily Araneinae have high richness and diversity than Argiopinae and Gasteracanthinae. The phylosymbiotic analysis revealed that there is no congruence between the gut bacteria composition dendrogram with their host phylogeny.
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spelling pubmed-84591222021-09-28 Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis Tyagi, Kaomud Tyagi, Inderjeet Kumar, Vikas Saudi J Biol Sci Original Article In the present study, an effort has been made to elucidate the gut bacterial diversity of twelve species of the family Araneidae under three subfamilies collected from 5 states of India along with their predicted metabolic role in functional metabolism. Further, we also compared the host species phylogeny based on partial cytochrome c oxidase subunit I (COI) sequences with the gut bacteria composition dendrogram to decipher the phylosymbiotic relationships. Analysis revealed the presence of 22 bacterial phyla, 145 families, and 364 genera in the gut, with Proteobacteria, Firmicutes, Actinobacteria, and Deinococcus-Thermus as the highest abundant phyla. Moreover, phylum Bacteriodetes was dominated only in Cyclosa mulmeinensis and Chlamydiae in Neoscona bengalensis. At the genus level, Bacillus, Acinetobacter, Cutibacterium, Pseudomonas, and Staphylococcus were the most dominant genera. Furthermore, the genus Prevotella was observed only in Cyclosa mulmeinensis, and endosymbiont Wolbachia only in Eriovixia laglaizei. The differential abundance analysis (DeSeq2) revealed the 19 significant ASVs represented by the genera like Acinetobacter, Vagoccoccus, Prevotella, Staphylococcus, Curvibacter, Corynebacterium, Paracoccus, Streptococcus, Microbacterium, and Pseudocitrobacter. The inter- and intra-subfamilies comparison based on diversity indices (alpha and beta diversity) revealed that the subfamily Araneinae have high richness and diversity than Argiopinae and Gasteracanthinae. The phylosymbiotic analysis revealed that there is no congruence between the gut bacteria composition dendrogram with their host phylogeny. Elsevier 2021-10 2021-06-24 /pmc/articles/PMC8459122/ /pubmed/34588907 http://dx.doi.org/10.1016/j.sjbs.2021.06.059 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Tyagi, Kaomud
Tyagi, Inderjeet
Kumar, Vikas
Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
title Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
title_full Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
title_fullStr Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
title_full_unstemmed Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
title_short Insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
title_sort insights into the gut bacterial communities of spider from wild with no evidence of phylosymbiosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459122/
https://www.ncbi.nlm.nih.gov/pubmed/34588907
http://dx.doi.org/10.1016/j.sjbs.2021.06.059
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