Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples

Standard diagnoses of SARS-CoV-2 infections are done by RNA extraction and real-time RT-PCR (rRT-PCR). However, the need for RNA extraction complicates testing due to increased processing time, high cost, and limited availability of commercial kits. Therefore, alternative methods for rRT-PCR detecti...

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Detalles Bibliográficos
Autores principales: Villota, Stephany D., Nipaz, Victoria E., Carrazco-Montalvo, Andrés, Hernandez, Sarah, Waggoner, Jesse J., Ponce, Patricio, Coloma, Josefina, Orlando, Alberto, Cevallos, Varsovia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier B.V. 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459549/
https://www.ncbi.nlm.nih.gov/pubmed/34563582
http://dx.doi.org/10.1016/j.jviromet.2021.114302

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459549/
https://www.ncbi.nlm.nih.gov/pubmed/34563582
http://dx.doi.org/10.1016/j.jviromet.2021.114302

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