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Alternative RNA extraction-free techniques for the real-time RT-PCR detection of SARS-CoV-2 in nasopharyngeal swab and sputum samples
Standard diagnoses of SARS-CoV-2 infections are done by RNA extraction and real-time RT-PCR (rRT-PCR). However, the need for RNA extraction complicates testing due to increased processing time, high cost, and limited availability of commercial kits. Therefore, alternative methods for rRT-PCR detecti...
Autores principales: | Villota, Stephany D., Nipaz, Victoria E., Carrazco-Montalvo, Andrés, Hernandez, Sarah, Waggoner, Jesse J., Ponce, Patricio, Coloma, Josefina, Orlando, Alberto, Cevallos, Varsovia |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier B.V.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459549/ https://www.ncbi.nlm.nih.gov/pubmed/34563582 http://dx.doi.org/10.1016/j.jviromet.2021.114302 |
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