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Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice

Hematopoietic stem progenitor cells (HSPCs) mobilized to peripheral blood, rather than those remaining in the bone marrow (BM), are commonly used as stem cell source in the clinic. As reactive oxygen species (ROS) are suggested as mediator of HSPC mobilization, we examined the impacts of glucose oxi...

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Autores principales: So, Han‐Sol, Kim, Min‐Guk, Lee, Jeong‐Chae, Kook, Sung‐Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459634/
https://www.ncbi.nlm.nih.gov/pubmed/34160898
http://dx.doi.org/10.1002/sctm.20-0514
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author So, Han‐Sol
Kim, Min‐Guk
Lee, Jeong‐Chae
Kook, Sung‐Ho
author_facet So, Han‐Sol
Kim, Min‐Guk
Lee, Jeong‐Chae
Kook, Sung‐Ho
author_sort So, Han‐Sol
collection PubMed
description Hematopoietic stem progenitor cells (HSPCs) mobilized to peripheral blood, rather than those remaining in the bone marrow (BM), are commonly used as stem cell source in the clinic. As reactive oxygen species (ROS) are suggested as mediator of HSPC mobilization, we examined the impacts of glucose oxidase (GO) on peripheral mobilization of BM HSPCs and the associated mechanisms. Intravenous injection of GO induced HSPC mobilization even by single treatment, and the GO‐mobilized cells maintained their long‐term reconstituting and differentiating potentials in conditioned recipients. GO‐injected mice lived a normal life without adverse effects such as stem cell senescence, hematopoietic disorders, and blood parameter alteration. The mobilization effect of GO was even evident in animal models showing poor mobilization, such as old, 5‐fluorouracil‐treated, or alendronate‐treated mice. Importantly, combined injection of GO with granulocyte colony‐stimulating factor (G‐CSF) and/or AMD3100 enhanced more greatly HSPC mobilization than did G‐CSF, AMD3100, or both. The GO‐stimulated HSPC mobilization was almost completely attenuated by n‐acetyl‐L‐cysteine treatment. Collectively, our results not only highlight the potential role of GO in HSPC mobilization via ROS signaling, but also provide a GO‐based new strategy to improve HSPC mobilization in poorly mobilizing allogeneic or autologous donors via combination with G‐CSF and/or AMD3100.
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spelling pubmed-84596342021-09-28 Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice So, Han‐Sol Kim, Min‐Guk Lee, Jeong‐Chae Kook, Sung‐Ho Stem Cells Transl Med Tissue‐specific Progenitor and Stem Cells Hematopoietic stem progenitor cells (HSPCs) mobilized to peripheral blood, rather than those remaining in the bone marrow (BM), are commonly used as stem cell source in the clinic. As reactive oxygen species (ROS) are suggested as mediator of HSPC mobilization, we examined the impacts of glucose oxidase (GO) on peripheral mobilization of BM HSPCs and the associated mechanisms. Intravenous injection of GO induced HSPC mobilization even by single treatment, and the GO‐mobilized cells maintained their long‐term reconstituting and differentiating potentials in conditioned recipients. GO‐injected mice lived a normal life without adverse effects such as stem cell senescence, hematopoietic disorders, and blood parameter alteration. The mobilization effect of GO was even evident in animal models showing poor mobilization, such as old, 5‐fluorouracil‐treated, or alendronate‐treated mice. Importantly, combined injection of GO with granulocyte colony‐stimulating factor (G‐CSF) and/or AMD3100 enhanced more greatly HSPC mobilization than did G‐CSF, AMD3100, or both. The GO‐stimulated HSPC mobilization was almost completely attenuated by n‐acetyl‐L‐cysteine treatment. Collectively, our results not only highlight the potential role of GO in HSPC mobilization via ROS signaling, but also provide a GO‐based new strategy to improve HSPC mobilization in poorly mobilizing allogeneic or autologous donors via combination with G‐CSF and/or AMD3100. John Wiley & Sons, Inc. 2021-06-23 /pmc/articles/PMC8459634/ /pubmed/34160898 http://dx.doi.org/10.1002/sctm.20-0514 Text en © 2021 The Authors. stem cells translational medicine published by Wiley Periodicals LLC on behalf of AlphaMed Press. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Tissue‐specific Progenitor and Stem Cells
So, Han‐Sol
Kim, Min‐Guk
Lee, Jeong‐Chae
Kook, Sung‐Ho
Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
title Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
title_full Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
title_fullStr Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
title_full_unstemmed Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
title_short Glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
title_sort glucose oxidase induces mobilization of long‐term repopulating hematopoietic cells in mice
topic Tissue‐specific Progenitor and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459634/
https://www.ncbi.nlm.nih.gov/pubmed/34160898
http://dx.doi.org/10.1002/sctm.20-0514
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