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Orf165 is associated with cytoplasmic male sterility in pepper
Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, co...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sociedade Brasileira de Genética
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459829/ https://www.ncbi.nlm.nih.gov/pubmed/34555144 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0030 |
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author | Wen, Jin-fen Zhao, Kai Lv, Jun-heng Huo, Jin-long Wang, Zi-ran Wan, Hong-jian Zhu, Hai-shan Zhang, Zhu-qing Shao, Gui-fang Wang, Jiao Zhang, Shui Yang, Ting-yu Zhang, Jing-rou Zou, Xue-xiao Deng, Ming-hua |
author_facet | Wen, Jin-fen Zhao, Kai Lv, Jun-heng Huo, Jin-long Wang, Zi-ran Wan, Hong-jian Zhu, Hai-shan Zhang, Zhu-qing Shao, Gui-fang Wang, Jiao Zhang, Shui Yang, Ting-yu Zhang, Jing-rou Zou, Xue-xiao Deng, Ming-hua |
author_sort | Wen, Jin-fen |
collection | PubMed |
description | Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper. |
format | Online Article Text |
id | pubmed-8459829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Sociedade Brasileira de Genética |
record_format | MEDLINE/PubMed |
spelling | pubmed-84598292021-10-01 Orf165 is associated with cytoplasmic male sterility in pepper Wen, Jin-fen Zhao, Kai Lv, Jun-heng Huo, Jin-long Wang, Zi-ran Wan, Hong-jian Zhu, Hai-shan Zhang, Zhu-qing Shao, Gui-fang Wang, Jiao Zhang, Shui Yang, Ting-yu Zhang, Jing-rou Zou, Xue-xiao Deng, Ming-hua Genet Mol Biol Plant Genetics Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper. Sociedade Brasileira de Genética 2021-09-22 /pmc/articles/PMC8459829/ /pubmed/34555144 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0030 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Plant Genetics Wen, Jin-fen Zhao, Kai Lv, Jun-heng Huo, Jin-long Wang, Zi-ran Wan, Hong-jian Zhu, Hai-shan Zhang, Zhu-qing Shao, Gui-fang Wang, Jiao Zhang, Shui Yang, Ting-yu Zhang, Jing-rou Zou, Xue-xiao Deng, Ming-hua Orf165 is associated with cytoplasmic male sterility in pepper |
title | Orf165 is associated with cytoplasmic male sterility in pepper |
title_full | Orf165 is associated with cytoplasmic male sterility in pepper |
title_fullStr | Orf165 is associated with cytoplasmic male sterility in pepper |
title_full_unstemmed | Orf165 is associated with cytoplasmic male sterility in pepper |
title_short | Orf165 is associated with cytoplasmic male sterility in pepper |
title_sort | orf165 is associated with cytoplasmic male sterility in pepper |
topic | Plant Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459829/ https://www.ncbi.nlm.nih.gov/pubmed/34555144 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0030 |
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