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Orf165 is associated with cytoplasmic male sterility in pepper

Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, co...

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Autores principales: Wen, Jin-fen, Zhao, Kai, Lv, Jun-heng, Huo, Jin-long, Wang, Zi-ran, Wan, Hong-jian, Zhu, Hai-shan, Zhang, Zhu-qing, Shao, Gui-fang, Wang, Jiao, Zhang, Shui, Yang, Ting-yu, Zhang, Jing-rou, Zou, Xue-xiao, Deng, Ming-hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Genética 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459829/
https://www.ncbi.nlm.nih.gov/pubmed/34555144
http://dx.doi.org/10.1590/1678-4685-GMB-2021-0030
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author Wen, Jin-fen
Zhao, Kai
Lv, Jun-heng
Huo, Jin-long
Wang, Zi-ran
Wan, Hong-jian
Zhu, Hai-shan
Zhang, Zhu-qing
Shao, Gui-fang
Wang, Jiao
Zhang, Shui
Yang, Ting-yu
Zhang, Jing-rou
Zou, Xue-xiao
Deng, Ming-hua
author_facet Wen, Jin-fen
Zhao, Kai
Lv, Jun-heng
Huo, Jin-long
Wang, Zi-ran
Wan, Hong-jian
Zhu, Hai-shan
Zhang, Zhu-qing
Shao, Gui-fang
Wang, Jiao
Zhang, Shui
Yang, Ting-yu
Zhang, Jing-rou
Zou, Xue-xiao
Deng, Ming-hua
author_sort Wen, Jin-fen
collection PubMed
description Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper.
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spelling pubmed-84598292021-10-01 Orf165 is associated with cytoplasmic male sterility in pepper Wen, Jin-fen Zhao, Kai Lv, Jun-heng Huo, Jin-long Wang, Zi-ran Wan, Hong-jian Zhu, Hai-shan Zhang, Zhu-qing Shao, Gui-fang Wang, Jiao Zhang, Shui Yang, Ting-yu Zhang, Jing-rou Zou, Xue-xiao Deng, Ming-hua Genet Mol Biol Plant Genetics Cytoplasmic male sterility (CMS) is a maternally inherited trait that derives from the inability to produce functional pollen in higher plants. CMS results from recombination of the mitochondrial genome. However, understanding of the molecular mechanism of CMS in pepper is limited. In this study, comparative transcriptomic analyses were performed using a near-isogenic CMS line 14A (CMS-14A) and a maintainer line 14B (ML-14B) as experimental materials. A total of 17,349 differentially expressed genes were detected between CMS-14A and ML-14B at the PMC meiosis stage. Among them, six unigenes associated with CMS and 108 unigenes involved in energy metabolism were identified. The gene orf165 was found in CMS-14A. When orf165 was introduced into ML-14B, almost 30% of transgenic plants were CMS. In addition, orf165 expression in transgenic CMS plants resulted in abnormal function of some genes involved in energy metabolism. When orf165 in transgenic CMS plant was silenced, the resulted orf165-silenced plant was male fertile and the expression patterns of some genes associated with energy metabolism were similar to ML-14B. Thus, we confirmed that orf165 influenced CMS in pepper. Sociedade Brasileira de Genética 2021-09-22 /pmc/articles/PMC8459829/ /pubmed/34555144 http://dx.doi.org/10.1590/1678-4685-GMB-2021-0030 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Plant Genetics
Wen, Jin-fen
Zhao, Kai
Lv, Jun-heng
Huo, Jin-long
Wang, Zi-ran
Wan, Hong-jian
Zhu, Hai-shan
Zhang, Zhu-qing
Shao, Gui-fang
Wang, Jiao
Zhang, Shui
Yang, Ting-yu
Zhang, Jing-rou
Zou, Xue-xiao
Deng, Ming-hua
Orf165 is associated with cytoplasmic male sterility in pepper
title Orf165 is associated with cytoplasmic male sterility in pepper
title_full Orf165 is associated with cytoplasmic male sterility in pepper
title_fullStr Orf165 is associated with cytoplasmic male sterility in pepper
title_full_unstemmed Orf165 is associated with cytoplasmic male sterility in pepper
title_short Orf165 is associated with cytoplasmic male sterility in pepper
title_sort orf165 is associated with cytoplasmic male sterility in pepper
topic Plant Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459829/
https://www.ncbi.nlm.nih.gov/pubmed/34555144
http://dx.doi.org/10.1590/1678-4685-GMB-2021-0030
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