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Bracoviruses recruit host integrases for their integration into caterpillar’s genome
Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles i...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460044/ https://www.ncbi.nlm.nih.gov/pubmed/34492000 http://dx.doi.org/10.1371/journal.pgen.1009751 |
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author | Wang, Zehua Ye, Xiqian Zhou, Yuenan Wu, Xiaotong Hu, Rongmin Zhu, Jiachen Chen, Ting Huguet, Elisabeth Shi, Min Drezen, Jean-Michel Huang, Jianhua Chen, Xuexin |
author_facet | Wang, Zehua Ye, Xiqian Zhou, Yuenan Wu, Xiaotong Hu, Rongmin Zhu, Jiachen Chen, Ting Huguet, Elisabeth Shi, Min Drezen, Jean-Michel Huang, Jianhua Chen, Xuexin |
author_sort | Wang, Zehua |
collection | PubMed |
description | Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential “8+5” nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar’s genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts. |
format | Online Article Text |
id | pubmed-8460044 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-84600442021-09-24 Bracoviruses recruit host integrases for their integration into caterpillar’s genome Wang, Zehua Ye, Xiqian Zhou, Yuenan Wu, Xiaotong Hu, Rongmin Zhu, Jiachen Chen, Ting Huguet, Elisabeth Shi, Min Drezen, Jean-Michel Huang, Jianhua Chen, Xuexin PLoS Genet Research Article Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential “8+5” nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar’s genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts. Public Library of Science 2021-09-07 /pmc/articles/PMC8460044/ /pubmed/34492000 http://dx.doi.org/10.1371/journal.pgen.1009751 Text en © 2021 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Wang, Zehua Ye, Xiqian Zhou, Yuenan Wu, Xiaotong Hu, Rongmin Zhu, Jiachen Chen, Ting Huguet, Elisabeth Shi, Min Drezen, Jean-Michel Huang, Jianhua Chen, Xuexin Bracoviruses recruit host integrases for their integration into caterpillar’s genome |
title | Bracoviruses recruit host integrases for their integration into caterpillar’s genome |
title_full | Bracoviruses recruit host integrases for their integration into caterpillar’s genome |
title_fullStr | Bracoviruses recruit host integrases for their integration into caterpillar’s genome |
title_full_unstemmed | Bracoviruses recruit host integrases for their integration into caterpillar’s genome |
title_short | Bracoviruses recruit host integrases for their integration into caterpillar’s genome |
title_sort | bracoviruses recruit host integrases for their integration into caterpillar’s genome |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460044/ https://www.ncbi.nlm.nih.gov/pubmed/34492000 http://dx.doi.org/10.1371/journal.pgen.1009751 |
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