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Bracoviruses recruit host integrases for their integration into caterpillar’s genome

Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles i...

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Autores principales: Wang, Zehua, Ye, Xiqian, Zhou, Yuenan, Wu, Xiaotong, Hu, Rongmin, Zhu, Jiachen, Chen, Ting, Huguet, Elisabeth, Shi, Min, Drezen, Jean-Michel, Huang, Jianhua, Chen, Xuexin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460044/
https://www.ncbi.nlm.nih.gov/pubmed/34492000
http://dx.doi.org/10.1371/journal.pgen.1009751
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author Wang, Zehua
Ye, Xiqian
Zhou, Yuenan
Wu, Xiaotong
Hu, Rongmin
Zhu, Jiachen
Chen, Ting
Huguet, Elisabeth
Shi, Min
Drezen, Jean-Michel
Huang, Jianhua
Chen, Xuexin
author_facet Wang, Zehua
Ye, Xiqian
Zhou, Yuenan
Wu, Xiaotong
Hu, Rongmin
Zhu, Jiachen
Chen, Ting
Huguet, Elisabeth
Shi, Min
Drezen, Jean-Michel
Huang, Jianhua
Chen, Xuexin
author_sort Wang, Zehua
collection PubMed
description Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential “8+5” nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar’s genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts.
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spelling pubmed-84600442021-09-24 Bracoviruses recruit host integrases for their integration into caterpillar’s genome Wang, Zehua Ye, Xiqian Zhou, Yuenan Wu, Xiaotong Hu, Rongmin Zhu, Jiachen Chen, Ting Huguet, Elisabeth Shi, Min Drezen, Jean-Michel Huang, Jianhua Chen, Xuexin PLoS Genet Research Article Some DNA viruses infect host animals usually by integrating their DNAs into the host genome. However, the mechanisms for integration remain largely unknown. Here, we find that Cotesia vestalis bracovirus (CvBV), a polydnavirus of the parasitic wasp C. vestalis (Haliday), integrates its DNA circles into host Plutella xylostella (L.) genome by two distinct strategies, conservatively and randomly, through high-throughput sequencing analysis. We confirmed that the conservatively integrating circles contain an essential “8+5” nucleotides motif which is required for integration. Then we find CvBV circles are integrated into the caterpillar’s genome in three temporal patterns, the early, mid and late stage-integration. We further identify that three CvBV-encoded integrases are responsible for some, but not all of the virus circle integrations, indeed they mainly participate in the processes of early stage-integration. Strikingly, we find two P. xylostella retroviral integrases (PxIN1 and PxIN2) are highly induced upon wasp parasitism, and PxIN1 is crucial for integration of some other early-integrated CvBV circles, such as CvBV_04, CvBV_12 and CvBV_24, while PxIN2 is important for integration of a late-integrated CvBV circle, CvBV_21. Our data uncover a novel mechanism in which CvBV integrates into the infected host genome, not only by utilizing its own integrases, but also by recruiting host enzymes. These findings will strongly deepen our understanding of how bracoviruses regulate and integrate into their hosts. Public Library of Science 2021-09-07 /pmc/articles/PMC8460044/ /pubmed/34492000 http://dx.doi.org/10.1371/journal.pgen.1009751 Text en © 2021 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Zehua
Ye, Xiqian
Zhou, Yuenan
Wu, Xiaotong
Hu, Rongmin
Zhu, Jiachen
Chen, Ting
Huguet, Elisabeth
Shi, Min
Drezen, Jean-Michel
Huang, Jianhua
Chen, Xuexin
Bracoviruses recruit host integrases for their integration into caterpillar’s genome
title Bracoviruses recruit host integrases for their integration into caterpillar’s genome
title_full Bracoviruses recruit host integrases for their integration into caterpillar’s genome
title_fullStr Bracoviruses recruit host integrases for their integration into caterpillar’s genome
title_full_unstemmed Bracoviruses recruit host integrases for their integration into caterpillar’s genome
title_short Bracoviruses recruit host integrases for their integration into caterpillar’s genome
title_sort bracoviruses recruit host integrases for their integration into caterpillar’s genome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460044/
https://www.ncbi.nlm.nih.gov/pubmed/34492000
http://dx.doi.org/10.1371/journal.pgen.1009751
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