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A discussion of syndromic molecular testing for clinical care

Current molecular detection methods for single or multiplex pathogens by real-time PCR generally offer great sensitivity and specificity. However, many infectious pathogens often result in very similar clinical presentations, complicating the test-order for physicians who have to narrow down the cau...

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Autores principales: Cassidy, Hayley, Van Genne, Mart, Lizarazo-Forero, Erley, Gard, Lilli, Niesters, Hubert G M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460109/
https://www.ncbi.nlm.nih.gov/pubmed/34555161
http://dx.doi.org/10.1093/jac/dkab243
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author Cassidy, Hayley
Van Genne, Mart
Lizarazo-Forero, Erley
Gard, Lilli
Niesters, Hubert G M
author_facet Cassidy, Hayley
Van Genne, Mart
Lizarazo-Forero, Erley
Gard, Lilli
Niesters, Hubert G M
author_sort Cassidy, Hayley
collection PubMed
description Current molecular detection methods for single or multiplex pathogens by real-time PCR generally offer great sensitivity and specificity. However, many infectious pathogens often result in very similar clinical presentations, complicating the test-order for physicians who have to narrow down the causative agent prior to in-house PCR testing. As a consequence, the intuitive response is to start empirical therapy to treat a broad spectrum of possible pathogens. Syndromic molecular testing has been increasingly integrated into routine clinical care, either to provide diagnostic, epidemiological or patient management information. These multiplex panels can be used to screen for predefined infectious disease pathogens simultaneously within a 1 h timeframe, creating opportunities for rapid diagnostics. Conversely, syndromic panels have their own challenges and must be adaptable to the evolving demands of the clinical setting. Firstly, questions have been raised regarding the clinical relevance of some of the targets included in the panels and secondly, there is the added expense of integration into the clinical laboratory. Here, we aim to discuss some of the factors that should be considered before performing syndromic testing rather than traditional low-plex in-house PCR.
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spelling pubmed-84601092021-09-24 A discussion of syndromic molecular testing for clinical care Cassidy, Hayley Van Genne, Mart Lizarazo-Forero, Erley Gard, Lilli Niesters, Hubert G M J Antimicrob Chemother Supplement Papers Current molecular detection methods for single or multiplex pathogens by real-time PCR generally offer great sensitivity and specificity. However, many infectious pathogens often result in very similar clinical presentations, complicating the test-order for physicians who have to narrow down the causative agent prior to in-house PCR testing. As a consequence, the intuitive response is to start empirical therapy to treat a broad spectrum of possible pathogens. Syndromic molecular testing has been increasingly integrated into routine clinical care, either to provide diagnostic, epidemiological or patient management information. These multiplex panels can be used to screen for predefined infectious disease pathogens simultaneously within a 1 h timeframe, creating opportunities for rapid diagnostics. Conversely, syndromic panels have their own challenges and must be adaptable to the evolving demands of the clinical setting. Firstly, questions have been raised regarding the clinical relevance of some of the targets included in the panels and secondly, there is the added expense of integration into the clinical laboratory. Here, we aim to discuss some of the factors that should be considered before performing syndromic testing rather than traditional low-plex in-house PCR. Oxford University Press 2021-09-23 /pmc/articles/PMC8460109/ /pubmed/34555161 http://dx.doi.org/10.1093/jac/dkab243 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Supplement Papers
Cassidy, Hayley
Van Genne, Mart
Lizarazo-Forero, Erley
Gard, Lilli
Niesters, Hubert G M
A discussion of syndromic molecular testing for clinical care
title A discussion of syndromic molecular testing for clinical care
title_full A discussion of syndromic molecular testing for clinical care
title_fullStr A discussion of syndromic molecular testing for clinical care
title_full_unstemmed A discussion of syndromic molecular testing for clinical care
title_short A discussion of syndromic molecular testing for clinical care
title_sort discussion of syndromic molecular testing for clinical care
topic Supplement Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460109/
https://www.ncbi.nlm.nih.gov/pubmed/34555161
http://dx.doi.org/10.1093/jac/dkab243
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