First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity

The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatell...

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Autores principales: Gwiazdowska, Aleksandra, Karpińska, Oliwia, Kamionka-Kanclerska, Katarzyna, Rowiński, Patryk, Panagiotopoulou, Hanna, Pomorski, Jan J., Broughton, Richard K., da Silva, Luis F. P., Rutkowski, Robert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460626/
https://www.ncbi.nlm.nih.gov/pubmed/34556712
http://dx.doi.org/10.1038/s41598-021-98364-3
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author Gwiazdowska, Aleksandra
Karpińska, Oliwia
Kamionka-Kanclerska, Katarzyna
Rowiński, Patryk
Panagiotopoulou, Hanna
Pomorski, Jan J.
Broughton, Richard K.
da Silva, Luis F. P.
Rutkowski, Robert
author_facet Gwiazdowska, Aleksandra
Karpińska, Oliwia
Kamionka-Kanclerska, Katarzyna
Rowiński, Patryk
Panagiotopoulou, Hanna
Pomorski, Jan J.
Broughton, Richard K.
da Silva, Luis F. P.
Rutkowski, Robert
author_sort Gwiazdowska, Aleksandra
collection PubMed
description The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland’s Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin.
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spelling pubmed-84606262021-09-24 First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity Gwiazdowska, Aleksandra Karpińska, Oliwia Kamionka-Kanclerska, Katarzyna Rowiński, Patryk Panagiotopoulou, Hanna Pomorski, Jan J. Broughton, Richard K. da Silva, Luis F. P. Rutkowski, Robert Sci Rep Article The European Robin is a small passerine bird associated with woodlands of Eurasia and North Africa. Despite being relatively widespread and common, little is known of the species’ breeding biology and genetic diversity. We used Next Generation Sequencing (NGS) to develop and characterize microsatellite markers for the European Robin, designing three multiplex panels to amplify 14 microsatellite loci. The level of polymorphism and its value for assessing parentage and genetic structure was estimated based on 119 individuals, including seven full families and 69 unrelated individuals form Poland’s Białowieża Primaeval Forest and an additional location in Portugal. All markers appeared to be highly variable. Analysis at the family level confirmed a Mendelian manner of inheritance in the investigated loci. Genetic data also revealed evidence for extra-pair paternity in one family. The set of markers that we developed are proven to be valuable for analysis of the breeding biology and population genetics of the European Robin. Nature Publishing Group UK 2021-09-23 /pmc/articles/PMC8460626/ /pubmed/34556712 http://dx.doi.org/10.1038/s41598-021-98364-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gwiazdowska, Aleksandra
Karpińska, Oliwia
Kamionka-Kanclerska, Katarzyna
Rowiński, Patryk
Panagiotopoulou, Hanna
Pomorski, Jan J.
Broughton, Richard K.
da Silva, Luis F. P.
Rutkowski, Robert
First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_full First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_fullStr First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_full_unstemmed First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_short First microsatellite markers for the European Robin (Erithacus rubecula) and their application in analysis of parentage and genetic diversity
title_sort first microsatellite markers for the european robin (erithacus rubecula) and their application in analysis of parentage and genetic diversity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460626/
https://www.ncbi.nlm.nih.gov/pubmed/34556712
http://dx.doi.org/10.1038/s41598-021-98364-3
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