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Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust

Bacterial communities in soil serve an important role in controlling terrestrial biogeochemical cycles and ecosystem processes. Increased nitrogen (N) deposition in Northwest China is generating quantifiable changes in many elements of the desert environment, but the impacts of N deposition, as well...

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Autores principales: Huang, Tingwen, Liu, Weiguo, Long, Xi-En, Jia, Yangyang, Wang, Xiyuan, Chen, Yinguang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460773/
https://www.ncbi.nlm.nih.gov/pubmed/34566900
http://dx.doi.org/10.3389/fmicb.2021.665975
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author Huang, Tingwen
Liu, Weiguo
Long, Xi-En
Jia, Yangyang
Wang, Xiyuan
Chen, Yinguang
author_facet Huang, Tingwen
Liu, Weiguo
Long, Xi-En
Jia, Yangyang
Wang, Xiyuan
Chen, Yinguang
author_sort Huang, Tingwen
collection PubMed
description Bacterial communities in soil serve an important role in controlling terrestrial biogeochemical cycles and ecosystem processes. Increased nitrogen (N) deposition in Northwest China is generating quantifiable changes in many elements of the desert environment, but the impacts of N deposition, as well as seasonal variations, on soil bacterial community composition and structure are poorly understood. We used high-throughput sequencing of bacterial 16S rRNA genes from Gurbantünggüt Desert moss crust soils to study the impacts of N addition on soil bacterial communities in March, May, and November. In November, we discovered that the OTU richness and diversity of soil bacterial community dropped linearly with increasing N input. In November and March, the diversity of the soil bacterial community decreased significantly in the medium-N and high-N treatments. In May, N addition caused a substantial change in the makeup of the soil bacterial composition, while the impacts were far less apparent in November and March. Furthermore, the relative abundance of major bacterial phyla reacted non-linearly to N addition, with high-N additions decreasing the relative richness of Proteobacteria, Bacteroidetes, and Acidobacteria while increasing the relative abundance of Actinobacteria and Chloroflexi. We also discovered that seasonality, as characterized by changes in soil moisture, pH, SOC, and AK content, had a significant impact on soil bacterial communities. Significant variations in the makeup of the community were discovered at the phylum and genus levels throughout the various months. In May, the variety of soil bacterial community was at its peak. Further investigation showed that the decrease in soil bacterial diversity was mostly attributed to a drop in soil pH. These results indicated that the impact of N deposition on the soil bacterial community was seasonally dependent, suggesting that future research should evaluate more than one sample season at the same time.
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spelling pubmed-84607732021-09-25 Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust Huang, Tingwen Liu, Weiguo Long, Xi-En Jia, Yangyang Wang, Xiyuan Chen, Yinguang Front Microbiol Microbiology Bacterial communities in soil serve an important role in controlling terrestrial biogeochemical cycles and ecosystem processes. Increased nitrogen (N) deposition in Northwest China is generating quantifiable changes in many elements of the desert environment, but the impacts of N deposition, as well as seasonal variations, on soil bacterial community composition and structure are poorly understood. We used high-throughput sequencing of bacterial 16S rRNA genes from Gurbantünggüt Desert moss crust soils to study the impacts of N addition on soil bacterial communities in March, May, and November. In November, we discovered that the OTU richness and diversity of soil bacterial community dropped linearly with increasing N input. In November and March, the diversity of the soil bacterial community decreased significantly in the medium-N and high-N treatments. In May, N addition caused a substantial change in the makeup of the soil bacterial composition, while the impacts were far less apparent in November and March. Furthermore, the relative abundance of major bacterial phyla reacted non-linearly to N addition, with high-N additions decreasing the relative richness of Proteobacteria, Bacteroidetes, and Acidobacteria while increasing the relative abundance of Actinobacteria and Chloroflexi. We also discovered that seasonality, as characterized by changes in soil moisture, pH, SOC, and AK content, had a significant impact on soil bacterial communities. Significant variations in the makeup of the community were discovered at the phylum and genus levels throughout the various months. In May, the variety of soil bacterial community was at its peak. Further investigation showed that the decrease in soil bacterial diversity was mostly attributed to a drop in soil pH. These results indicated that the impact of N deposition on the soil bacterial community was seasonally dependent, suggesting that future research should evaluate more than one sample season at the same time. Frontiers Media S.A. 2021-09-10 /pmc/articles/PMC8460773/ /pubmed/34566900 http://dx.doi.org/10.3389/fmicb.2021.665975 Text en Copyright © 2021 Huang, Liu, Long, Jia, Wang and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Huang, Tingwen
Liu, Weiguo
Long, Xi-En
Jia, Yangyang
Wang, Xiyuan
Chen, Yinguang
Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust
title Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust
title_full Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust
title_fullStr Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust
title_full_unstemmed Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust
title_short Different Responses of Soil Bacterial Communities to Nitrogen Addition in Moss Crust
title_sort different responses of soil bacterial communities to nitrogen addition in moss crust
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460773/
https://www.ncbi.nlm.nih.gov/pubmed/34566900
http://dx.doi.org/10.3389/fmicb.2021.665975
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