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Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study

Percutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents, partly e...

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Autores principales: García-Vidal, José Antonio, Salinas, Jesús, Escolar-Reina, Pilar, Cuello, Francisco, Ortega, Nieves, de Dios Berná-Mestre, Juan, López-Nicolás, Manuel, Valera-Garrido, Fermín, Medina-Mirapeix, Francesc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460800/
https://www.ncbi.nlm.nih.gov/pubmed/34556763
http://dx.doi.org/10.1038/s41598-021-98451-5
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author García-Vidal, José Antonio
Salinas, Jesús
Escolar-Reina, Pilar
Cuello, Francisco
Ortega, Nieves
de Dios Berná-Mestre, Juan
López-Nicolás, Manuel
Valera-Garrido, Fermín
Medina-Mirapeix, Francesc
author_facet García-Vidal, José Antonio
Salinas, Jesús
Escolar-Reina, Pilar
Cuello, Francisco
Ortega, Nieves
de Dios Berná-Mestre, Juan
López-Nicolás, Manuel
Valera-Garrido, Fermín
Medina-Mirapeix, Francesc
author_sort García-Vidal, José Antonio
collection PubMed
description Percutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents, partly explained by the changes in pH that it generates. However, these effects have not yet been demonstrated. The aim of this study was to evaluate the bactericidal effect and the changes in pH caused by PNE. S. aureus were prepared in two different solutions (TSB and saline solution) and in different concentrations (from 9 to 6 Log(10) CFU/mL). Bacteria were treated with three experimental PNE doses to assess bacterial death levels and the changes caused to the pH of the medium. The viable cell count showed that all experimental PNE doses had a bactericidal effect against a high concentration (9 Log(10) CFU/mL) of S. aureus in saline solution (p < 0.001). Furthermore, we found that when the concentration of bacteria decreased, a lower dose of galvanic current generated the same effect as a higher dose. Changes in pH were registered only in experiments performed with saline solution. PNE had a bactericidal effect against S. aureus and the level of this effect was mainly modulated by the solution, the bacterial concentration and the dose. Changes affecting pH were modulated by the type of solution and there was no relationship between this and bacterial death.
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spelling pubmed-84608002021-09-27 Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study García-Vidal, José Antonio Salinas, Jesús Escolar-Reina, Pilar Cuello, Francisco Ortega, Nieves de Dios Berná-Mestre, Juan López-Nicolás, Manuel Valera-Garrido, Fermín Medina-Mirapeix, Francesc Sci Rep Article Percutaneous needle electrolysis (PNE) is a physiotherapy technique that has been shown to be effective in different pathologies such as tendinopathies or mammary fistula. For many years, theoretical bactericidal and germicidal effects have been attributed to this type of galvanic currents, partly explained by the changes in pH that it generates. However, these effects have not yet been demonstrated. The aim of this study was to evaluate the bactericidal effect and the changes in pH caused by PNE. S. aureus were prepared in two different solutions (TSB and saline solution) and in different concentrations (from 9 to 6 Log(10) CFU/mL). Bacteria were treated with three experimental PNE doses to assess bacterial death levels and the changes caused to the pH of the medium. The viable cell count showed that all experimental PNE doses had a bactericidal effect against a high concentration (9 Log(10) CFU/mL) of S. aureus in saline solution (p < 0.001). Furthermore, we found that when the concentration of bacteria decreased, a lower dose of galvanic current generated the same effect as a higher dose. Changes in pH were registered only in experiments performed with saline solution. PNE had a bactericidal effect against S. aureus and the level of this effect was mainly modulated by the solution, the bacterial concentration and the dose. Changes affecting pH were modulated by the type of solution and there was no relationship between this and bacterial death. Nature Publishing Group UK 2021-09-23 /pmc/articles/PMC8460800/ /pubmed/34556763 http://dx.doi.org/10.1038/s41598-021-98451-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
García-Vidal, José Antonio
Salinas, Jesús
Escolar-Reina, Pilar
Cuello, Francisco
Ortega, Nieves
de Dios Berná-Mestre, Juan
López-Nicolás, Manuel
Valera-Garrido, Fermín
Medina-Mirapeix, Francesc
Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
title Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
title_full Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
title_fullStr Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
title_full_unstemmed Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
title_short Galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
title_sort galvanic current dosage and bacterial concentration are determinants of the bactericidal effect of percutaneous needle electrolysis: an in vitro study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460800/
https://www.ncbi.nlm.nih.gov/pubmed/34556763
http://dx.doi.org/10.1038/s41598-021-98451-5
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